Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4462 and Psyr_4463 are separated by 41 nucleotides Psyr_4463 and Psyr_4464 are separated by 143 nucleotides Psyr_4464 and Psyr_4465 overlap by 4 nucleotides
Psyr_4462: Psyr_4462 - diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase, at 5,324,768 to 5,325,868
_4462
Psyr_4463: Psyr_4463 - Protein of unknown function DUF193, at 5,325,910 to 5,326,407
_4463
Psyr_4464: Psyr_4464 - lipoprotein, putative, at 5,326,551 to 5,327,000
_4464
Psyr_4465: Psyr_4465 - conserved domain protein, at 5,326,997 to 5,327,650
_4465
Position (kb) 5325
5326
5327 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5325.871 kb on - strand at 5325.871 kb on - strand at 5325.871 kb on - strand at 5325.871 kb on - strand at 5325.912 kb on - strand at 5325.912 kb on - strand at 5325.912 kb on - strand at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.301 kb on - strand, within Psyr_4463 at 5326.301 kb on - strand, within Psyr_4463 at 5326.450 kb on + strand at 5326.450 kb on + strand at 5326.450 kb on + strand at 5326.451 kb on - strand at 5326.451 kb on - strand at 5326.451 kb on - strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.465 kb on + strand at 5326.465 kb on + strand at 5326.497 kb on + strand at 5326.497 kb on + strand at 5326.498 kb on - strand at 5326.498 kb on - strand at 5326.498 kb on - strand at 5326.559 kb on - strand at 5326.574 kb on - strand at 5326.693 kb on - strand, within Psyr_4464 at 5327.169 kb on - strand, within Psyr_4465 at 5327.393 kb on + strand, within Psyr_4465
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 5,325,871 - -0.7 5,325,871 - -1.1 5,325,871 - -0.5 5,325,871 - +1.1 5,325,912 - -0.9 5,325,912 - -0.6 5,325,912 - -0.1 5,326,005 - Psyr_4463 0.19 -0.2 5,326,005 - Psyr_4463 0.19 +0.9 5,326,005 - Psyr_4463 0.19 +1.8 5,326,005 - Psyr_4463 0.19 +0.1 5,326,005 - Psyr_4463 0.19 +2.3 5,326,005 - Psyr_4463 0.19 -3.5 5,326,301 - Psyr_4463 0.79 -1.2 5,326,301 - Psyr_4463 0.79 -1.5 5,326,450 + +1.3 5,326,450 + -0.1 5,326,450 + +0.0 5,326,451 - -1.1 5,326,451 - -0.2 5,326,451 - -0.4 5,326,452 + -0.6 5,326,452 + -0.7 5,326,452 + -0.6 5,326,452 + +1.3 5,326,452 + +1.0 5,326,452 + -0.5 5,326,452 + -0.3 5,326,452 + -0.0 5,326,453 - -0.2 5,326,453 - +0.1 5,326,453 - +0.6 5,326,453 - +0.2 5,326,453 - -3.1 5,326,453 - -0.2 5,326,454 + +0.9 5,326,454 + -0.5 5,326,454 + -0.1 5,326,454 + +2.3 5,326,454 + -0.9 5,326,454 + -0.1 5,326,454 + -0.5 5,326,454 + +0.9 5,326,454 + +1.5 5,326,455 - +0.9 5,326,455 - -1.1 5,326,455 - +0.8 5,326,455 - +0.9 5,326,455 - +0.2 5,326,455 - +0.3 5,326,455 - -0.7 5,326,465 + -0.2 5,326,465 + +0.8 5,326,497 + +0.4 5,326,497 + +0.1 5,326,498 - +0.0 5,326,498 - -0.7 5,326,498 - +0.3 5,326,559 - +0.0 5,326,574 - -0.3 5,326,693 - Psyr_4464 0.32 -0.8 5,327,169 - Psyr_4465 0.26 -0.1 5,327,393 + Psyr_4465 0.61 -1.8
Or see this region's nucleotide sequence