Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3944 and Psyr_3945 are separated by 201 nucleotides Psyr_3945 and Psyr_3946 are separated by 100 nucleotides Psyr_3946 and Psyr_3947 overlap by 4 nucleotides
Psyr_3944: Psyr_3944 - ATP dependent PIM1 peptidase, Serine peptidase, MEROPS family S16, at 4,691,427 to 4,693,844
_3944
Psyr_3945: Psyr_3945 - secreted protein-like protein, at 4,694,046 to 4,694,438
_3945
Psyr_3946: Psyr_3946 - Proteobacterial methyltransferase, at 4,694,539 to 4,695,282
_3946
Psyr_3947: Psyr_3947 - Methyltransferase, putative, at 4,695,279 to 4,696,238
_3947
Position (kb) 4694
4695 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4693.382 kb on + strand, within Psyr_3944 at 4693.382 kb on + strand, within Psyr_3944 at 4693.382 kb on + strand, within Psyr_3944 at 4693.453 kb on - strand, within Psyr_3944 at 4693.458 kb on + strand, within Psyr_3944 at 4693.458 kb on + strand, within Psyr_3944 at 4693.458 kb on + strand, within Psyr_3944 at 4693.458 kb on + strand, within Psyr_3944 at 4693.458 kb on + strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.459 kb on - strand, within Psyr_3944 at 4693.525 kb on + strand, within Psyr_3944 at 4693.525 kb on + strand, within Psyr_3944 at 4693.525 kb on + strand, within Psyr_3944 at 4693.525 kb on + strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.526 kb on - strand, within Psyr_3944 at 4693.666 kb on + strand at 4693.666 kb on + strand at 4693.666 kb on + strand at 4693.666 kb on + strand at 4693.666 kb on + strand at 4693.666 kb on + strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.667 kb on - strand at 4693.776 kb on + strand at 4693.776 kb on + strand at 4693.777 kb on - strand at 4693.777 kb on - strand at 4693.843 kb on - strand at 4693.853 kb on - strand at 4693.853 kb on - strand at 4693.893 kb on + strand at 4694.025 kb on - strand at 4694.271 kb on + strand, within Psyr_3945 at 4694.271 kb on + strand, within Psyr_3945 at 4694.271 kb on + strand, within Psyr_3945 at 4694.271 kb on + strand, within Psyr_3945 at 4694.271 kb on + strand, within Psyr_3945 at 4694.271 kb on + strand, within Psyr_3945 at 4694.271 kb on + strand, within Psyr_3945 at 4694.272 kb on - strand, within Psyr_3945 at 4694.272 kb on - strand, within Psyr_3945 at 4694.272 kb on - strand, within Psyr_3945 at 4694.272 kb on - strand, within Psyr_3945 at 4694.272 kb on - strand, within Psyr_3945 at 4694.370 kb on + strand, within Psyr_3945 at 4694.370 kb on + strand, within Psyr_3945 at 4694.470 kb on - strand at 4694.723 kb on - strand, within Psyr_3946 at 4694.842 kb on + strand, within Psyr_3946 at 4694.843 kb on - strand, within Psyr_3946 at 4694.843 kb on - strand, within Psyr_3946 at 4694.843 kb on - strand, within Psyr_3946 at 4695.252 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 4,693,382 + Psyr_3944 0.81 +0.9 4,693,382 + Psyr_3944 0.81 +0.5 4,693,382 + Psyr_3944 0.81 +0.1 4,693,453 - Psyr_3944 0.84 +0.3 4,693,458 + Psyr_3944 0.84 -0.0 4,693,458 + Psyr_3944 0.84 +0.2 4,693,458 + Psyr_3944 0.84 -0.3 4,693,458 + Psyr_3944 0.84 +0.6 4,693,458 + Psyr_3944 0.84 -1.9 4,693,459 - Psyr_3944 0.84 -0.3 4,693,459 - Psyr_3944 0.84 -1.5 4,693,459 - Psyr_3944 0.84 +2.5 4,693,459 - Psyr_3944 0.84 -0.1 4,693,459 - Psyr_3944 0.84 +0.7 4,693,459 - Psyr_3944 0.84 -0.5 4,693,459 - Psyr_3944 0.84 +1.5 4,693,525 + Psyr_3944 0.87 +1.4 4,693,525 + Psyr_3944 0.87 -0.3 4,693,525 + Psyr_3944 0.87 -1.6 4,693,525 + Psyr_3944 0.87 +0.7 4,693,526 - Psyr_3944 0.87 -0.2 4,693,526 - Psyr_3944 0.87 -0.3 4,693,526 - Psyr_3944 0.87 +0.4 4,693,526 - Psyr_3944 0.87 -2.3 4,693,526 - Psyr_3944 0.87 +0.9 4,693,526 - Psyr_3944 0.87 -0.5 4,693,526 - Psyr_3944 0.87 -0.2 4,693,666 + +0.7 4,693,666 + -1.1 4,693,666 + +0.3 4,693,666 + +1.6 4,693,666 + -0.4 4,693,666 + +4.1 4,693,667 - +1.5 4,693,667 - -2.3 4,693,667 - +0.2 4,693,667 - -1.9 4,693,667 - +0.5 4,693,667 - -1.7 4,693,667 - -1.3 4,693,667 - +1.5 4,693,667 - +1.3 4,693,667 - +0.0 4,693,776 + -1.4 4,693,776 + +0.7 4,693,777 - -1.4 4,693,777 - -0.2 4,693,843 - +0.7 4,693,853 - -1.0 4,693,853 - -1.5 4,693,893 + -2.4 4,694,025 - -1.5 4,694,271 + Psyr_3945 0.57 +0.3 4,694,271 + Psyr_3945 0.57 +1.5 4,694,271 + Psyr_3945 0.57 -1.0 4,694,271 + Psyr_3945 0.57 -1.0 4,694,271 + Psyr_3945 0.57 -0.5 4,694,271 + Psyr_3945 0.57 +1.0 4,694,271 + Psyr_3945 0.57 -0.3 4,694,272 - Psyr_3945 0.58 -0.4 4,694,272 - Psyr_3945 0.58 -0.8 4,694,272 - Psyr_3945 0.58 -0.8 4,694,272 - Psyr_3945 0.58 -0.9 4,694,272 - Psyr_3945 0.58 -1.1 4,694,370 + Psyr_3945 0.82 +0.0 4,694,370 + Psyr_3945 0.82 -2.1 4,694,470 - +1.1 4,694,723 - Psyr_3946 0.25 -0.4 4,694,842 + Psyr_3946 0.41 +0.9 4,694,843 - Psyr_3946 0.41 +0.4 4,694,843 - Psyr_3946 0.41 -0.9 4,694,843 - Psyr_3946 0.41 -1.4 4,695,252 + -0.6
Or see this region's nucleotide sequence