Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3120 and Psyr_3121 are separated by 152 nucleotides Psyr_3121 and Psyr_3122 are separated by 42 nucleotides Psyr_3122 and Psyr_3123 are separated by 421 nucleotides
Psyr_3120: Psyr_3120 - D-glucarate dehydratase, at 3,740,656 to 3,742,008
_3120
Psyr_3121: Psyr_3121 - Protein of unknown function DUF1345, at 3,742,161 to 3,742,814
_3121
Psyr_3122: Psyr_3122 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 3,742,857 to 3,743,813
_3122
Psyr_3123: Psyr_3123 - type III effector HopAH2, at 3,744,235 to 3,745,377
_3123
Position (kb)
3742
3743
3744 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3741.907 kb on + strand at 3741.907 kb on + strand at 3741.908 kb on - strand at 3741.908 kb on - strand at 3742.104 kb on + strand at 3742.105 kb on - strand at 3742.105 kb on - strand at 3742.126 kb on + strand at 3742.127 kb on - strand at 3742.127 kb on - strand at 3742.127 kb on - strand at 3742.298 kb on - strand, within Psyr_3121 at 3742.578 kb on + strand, within Psyr_3121 at 3742.579 kb on - strand, within Psyr_3121 at 3742.579 kb on - strand, within Psyr_3121 at 3742.638 kb on + strand, within Psyr_3121 at 3742.654 kb on - strand, within Psyr_3121 at 3742.912 kb on + strand at 3742.913 kb on - strand at 3742.965 kb on + strand, within Psyr_3122 at 3742.976 kb on + strand, within Psyr_3122 at 3742.977 kb on - strand, within Psyr_3122 at 3742.977 kb on - strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.172 kb on - strand, within Psyr_3122 at 3743.172 kb on - strand, within Psyr_3122 at 3743.330 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.548 kb on - strand, within Psyr_3122 at 3743.719 kb on - strand at 3743.841 kb on + strand at 3743.841 kb on + strand at 3743.841 kb on + strand at 3743.842 kb on - strand at 3743.842 kb on - strand at 3743.842 kb on - strand at 3743.842 kb on - strand at 3743.842 kb on - strand at 3743.842 kb on - strand at 3743.842 kb on - strand at 3743.885 kb on - strand at 3743.974 kb on - strand at 3744.010 kb on + strand at 3744.010 kb on + strand at 3744.010 kb on + strand at 3744.010 kb on + strand at 3744.010 kb on + strand at 3744.011 kb on - strand at 3744.049 kb on + strand at 3744.049 kb on + strand at 3744.049 kb on + strand at 3744.049 kb on + strand at 3744.049 kb on + strand at 3744.049 kb on + strand at 3744.049 kb on + strand at 3744.050 kb on - strand at 3744.050 kb on - strand at 3744.050 kb on - strand at 3744.050 kb on - strand at 3744.077 kb on - strand at 3744.077 kb on - strand at 3744.117 kb on + strand at 3744.134 kb on + strand at 3744.134 kb on + strand at 3744.135 kb on - strand at 3744.135 kb on - strand at 3744.135 kb on - strand at 3744.135 kb on - strand at 3744.136 kb on + strand at 3744.136 kb on + strand at 3744.136 kb on + strand at 3744.136 kb on + strand at 3744.137 kb on - strand at 3744.160 kb on + strand at 3744.373 kb on + strand, within Psyr_3123 at 3744.373 kb on + strand, within Psyr_3123 at 3744.373 kb on + strand, within Psyr_3123 at 3744.373 kb on + strand, within Psyr_3123 at 3744.373 kb on + strand, within Psyr_3123 at 3744.373 kb on + strand, within Psyr_3123 at 3744.373 kb on + strand, within Psyr_3123 at 3744.374 kb on - strand, within Psyr_3123 at 3744.374 kb on - strand, within Psyr_3123 at 3744.374 kb on - strand, within Psyr_3123 at 3744.374 kb on - strand, within Psyr_3123 at 3744.374 kb on - strand, within Psyr_3123 at 3744.424 kb on + strand, within Psyr_3123 at 3744.424 kb on + strand, within Psyr_3123 at 3744.424 kb on + strand, within Psyr_3123 at 3744.425 kb on - strand, within Psyr_3123 at 3744.425 kb on - strand, within Psyr_3123 at 3744.426 kb on + strand, within Psyr_3123 at 3744.489 kb on + strand, within Psyr_3123 at 3744.490 kb on - strand, within Psyr_3123 at 3744.490 kb on - strand, within Psyr_3123 at 3744.741 kb on - strand, within Psyr_3123 at 3744.741 kb on - strand, within Psyr_3123
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 3,741,907 + +0.6 3,741,907 + +0.5 3,741,908 - +0.2 3,741,908 - -0.6 3,742,104 + -2.5 3,742,105 - +0.3 3,742,105 - +0.4 3,742,126 + -1.4 3,742,127 - +1.0 3,742,127 - +0.4 3,742,127 - -0.4 3,742,298 - Psyr_3121 0.21 +3.2 3,742,578 + Psyr_3121 0.64 +0.1 3,742,579 - Psyr_3121 0.64 +1.7 3,742,579 - Psyr_3121 0.64 +0.1 3,742,638 + Psyr_3121 0.73 +1.3 3,742,654 - Psyr_3121 0.75 -0.4 3,742,912 + +0.1 3,742,913 - +0.9 3,742,965 + Psyr_3122 0.11 +1.0 3,742,976 + Psyr_3122 0.12 +1.0 3,742,977 - Psyr_3122 0.13 +0.6 3,742,977 - Psyr_3122 0.13 +1.5 3,743,169 + Psyr_3122 0.33 -0.1 3,743,169 + Psyr_3122 0.33 -0.4 3,743,169 + Psyr_3122 0.33 +0.8 3,743,169 + Psyr_3122 0.33 +0.3 3,743,169 + +0.1 3,743,170 - Psyr_3122 0.33 +0.2 3,743,170 - Psyr_3122 0.33 -0.1 3,743,170 - Psyr_3122 0.33 +0.6 3,743,170 - Psyr_3122 0.33 -0.9 3,743,170 - Psyr_3122 0.33 -0.5 3,743,170 - Psyr_3122 0.33 +0.3 3,743,170 - Psyr_3122 0.33 -0.9 3,743,170 - Psyr_3122 0.33 +0.3 3,743,171 + Psyr_3122 0.33 +0.5 3,743,171 + Psyr_3122 0.33 +0.8 3,743,171 + Psyr_3122 0.33 +0.8 3,743,171 + Psyr_3122 0.33 -0.1 3,743,171 + Psyr_3122 0.33 +1.8 3,743,171 + Psyr_3122 0.33 +1.1 3,743,172 - Psyr_3122 0.33 +3.3 3,743,172 - Psyr_3122 0.33 +0.7 3,743,330 + Psyr_3122 0.49 -0.7 3,743,427 + Psyr_3122 0.60 +0.0 3,743,427 + Psyr_3122 0.60 -0.1 3,743,427 + Psyr_3122 0.60 +0.5 3,743,427 + Psyr_3122 0.60 +2.7 3,743,427 + Psyr_3122 0.60 +0.0 3,743,427 + Psyr_3122 0.60 +0.6 3,743,427 + Psyr_3122 0.60 +0.0 3,743,427 + Psyr_3122 0.60 +0.7 3,743,427 + Psyr_3122 0.60 +0.9 3,743,427 + Psyr_3122 0.60 +1.1 3,743,427 + Psyr_3122 0.60 -0.5 3,743,428 - Psyr_3122 0.60 -1.6 3,743,428 - Psyr_3122 0.60 -0.0 3,743,428 - Psyr_3122 0.60 +3.3 3,743,428 - Psyr_3122 0.60 +0.1 3,743,428 - Psyr_3122 0.60 -0.8 3,743,428 - Psyr_3122 0.60 +0.3 3,743,428 - Psyr_3122 0.60 +0.7 3,743,428 - Psyr_3122 0.60 -0.9 3,743,428 - Psyr_3122 0.60 -0.1 3,743,428 - Psyr_3122 0.60 +1.5 3,743,428 - Psyr_3122 0.60 +0.2 3,743,428 - Psyr_3122 0.60 -0.5 3,743,428 - Psyr_3122 0.60 +0.5 3,743,548 - Psyr_3122 0.72 +2.3 3,743,719 - -0.1 3,743,841 + +0.1 3,743,841 + -0.3 3,743,841 + +0.3 3,743,842 - -0.6 3,743,842 - -1.1 3,743,842 - -0.5 3,743,842 - -0.6 3,743,842 - -0.7 3,743,842 - +0.3 3,743,842 - -0.1 3,743,885 - -0.5 3,743,974 - -1.1 3,744,010 + -0.7 3,744,010 + +0.1 3,744,010 + -1.3 3,744,010 + -0.7 3,744,010 + -0.7 3,744,011 - -0.4 3,744,049 + -0.7 3,744,049 + -0.5 3,744,049 + +0.6 3,744,049 + -0.6 3,744,049 + -1.3 3,744,049 + -1.4 3,744,049 + -0.9 3,744,050 - -0.3 3,744,050 - +0.5 3,744,050 - +0.7 3,744,050 - -0.3 3,744,077 - -0.7 3,744,077 - -1.1 3,744,117 + +0.1 3,744,134 + +0.9 3,744,134 + -1.0 3,744,135 - -0.3 3,744,135 - -0.1 3,744,135 - +0.1 3,744,135 - -0.1 3,744,136 + -0.3 3,744,136 + +0.8 3,744,136 + +1.7 3,744,136 + -0.1 3,744,137 - +2.0 3,744,160 + -0.9 3,744,373 + Psyr_3123 0.12 +0.9 3,744,373 + Psyr_3123 0.12 -0.4 3,744,373 + Psyr_3123 0.12 +0.1 3,744,373 + Psyr_3123 0.12 +0.4 3,744,373 + Psyr_3123 0.12 +0.7 3,744,373 + Psyr_3123 0.12 +2.3 3,744,373 + Psyr_3123 0.12 -1.1 3,744,374 - Psyr_3123 0.12 +1.7 3,744,374 - Psyr_3123 0.12 -0.0 3,744,374 - Psyr_3123 0.12 +0.7 3,744,374 - Psyr_3123 0.12 -0.7 3,744,374 - Psyr_3123 0.12 +0.7 3,744,424 + Psyr_3123 0.17 -1.2 3,744,424 + Psyr_3123 0.17 -0.2 3,744,424 + Psyr_3123 0.17 +1.3 3,744,425 - Psyr_3123 0.17 +1.7 3,744,425 - Psyr_3123 0.17 -0.8 3,744,426 + Psyr_3123 0.17 -0.0 3,744,489 + Psyr_3123 0.22 +0.2 3,744,490 - Psyr_3123 0.22 +2.2 3,744,490 - Psyr_3123 0.22 +1.8 3,744,741 - Psyr_3123 0.44 +0.4 3,744,741 - Psyr_3123 0.44 -0.4
Or see this region's nucleotide sequence