Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2268 and Psyr_2269 are separated by 174 nucleotides Psyr_2269 and Psyr_2270 are separated by 11 nucleotides Psyr_2270 and Psyr_2271 are separated by 140 nucleotides
Psyr_2268: Psyr_2268 - transcriptional regulator, LysR family, at 2,634,007 to 2,634,915
_2268
Psyr_2269: Psyr_2269 - GTP cyclohydrolase I, at 2,635,090 to 2,635,995
_2269
Psyr_2270: Psyr_2270 - transferase hexapeptide repeat protein, at 2,636,007 to 2,636,567
_2270
Psyr_2271: Psyr_2271 - membrane protein, putative, at 2,636,708 to 2,637,874
_2271
Position (kb)
2635
2636 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2634.535 kb on - strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.587 kb on - strand, within Psyr_2268 at 2634.636 kb on + strand, within Psyr_2268 at 2634.636 kb on + strand, within Psyr_2268 at 2634.689 kb on - strand, within Psyr_2268 at 2634.689 kb on - strand, within Psyr_2268 at 2634.689 kb on - strand, within Psyr_2268 at 2634.811 kb on - strand, within Psyr_2268 at 2634.811 kb on - strand, within Psyr_2268 at 2634.950 kb on + strand at 2634.950 kb on + strand at 2634.950 kb on + strand at 2634.950 kb on + strand at 2634.951 kb on - strand at 2634.951 kb on - strand at 2634.954 kb on + strand at 2634.955 kb on - strand at 2634.955 kb on - strand at 2635.020 kb on + strand at 2635.020 kb on + strand at 2635.021 kb on - strand at 2635.275 kb on + strand, within Psyr_2269 at 2635.275 kb on + strand, within Psyr_2269 at 2635.275 kb on + strand, within Psyr_2269 at 2635.275 kb on + strand, within Psyr_2269 at 2635.276 kb on - strand, within Psyr_2269 at 2635.276 kb on - strand, within Psyr_2269 at 2635.543 kb on + strand, within Psyr_2269 at 2635.773 kb on - strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.984 kb on + strand at 2635.984 kb on + strand at 2635.985 kb on - strand at 2635.985 kb on - strand at 2636.059 kb on - strand at 2636.059 kb on - strand at 2636.067 kb on - strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.598 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.613 kb on - strand at 2636.668 kb on + strand at 2636.669 kb on - strand at 2636.669 kb on - strand at 2636.782 kb on + strand at 2636.782 kb on + strand at 2636.963 kb on + strand, within Psyr_2271 at 2636.964 kb on - strand, within Psyr_2271
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 2,634,535 - Psyr_2268 0.58 -0.4 2,634,586 + Psyr_2268 0.64 +1.6 2,634,586 + Psyr_2268 0.64 +0.8 2,634,586 + Psyr_2268 0.64 -1.5 2,634,586 + Psyr_2268 0.64 -0.5 2,634,587 - Psyr_2268 0.64 +0.5 2,634,636 + Psyr_2268 0.69 +1.1 2,634,636 + Psyr_2268 0.69 +1.5 2,634,689 - Psyr_2268 0.75 +0.2 2,634,689 - Psyr_2268 0.75 -0.3 2,634,689 - Psyr_2268 0.75 -0.1 2,634,811 - Psyr_2268 0.88 -1.2 2,634,811 - Psyr_2268 0.88 -1.2 2,634,950 + -0.9 2,634,950 + -0.2 2,634,950 + -1.3 2,634,950 + -0.2 2,634,951 - -1.3 2,634,951 - -0.9 2,634,954 + -0.7 2,634,955 - +2.8 2,634,955 - -1.2 2,635,020 + -0.7 2,635,020 + -0.9 2,635,021 - -2.0 2,635,275 + Psyr_2269 0.20 -1.5 2,635,275 + Psyr_2269 0.20 +0.0 2,635,275 + Psyr_2269 0.20 -0.5 2,635,275 + Psyr_2269 0.20 -0.4 2,635,276 - Psyr_2269 0.21 -0.4 2,635,276 - Psyr_2269 0.21 -0.6 2,635,543 + Psyr_2269 0.50 -0.0 2,635,773 - Psyr_2269 0.75 +2.4 2,635,861 + Psyr_2269 0.85 -1.4 2,635,861 + Psyr_2269 0.85 -1.0 2,635,861 + Psyr_2269 0.85 -1.0 2,635,861 + Psyr_2269 0.85 +0.5 2,635,984 + -0.1 2,635,984 + +1.0 2,635,985 - -2.2 2,635,985 - +0.8 2,636,059 - -2.1 2,636,059 - +0.6 2,636,067 - Psyr_2270 0.11 -0.2 2,636,418 + Psyr_2270 0.73 -2.5 2,636,418 + Psyr_2270 0.73 -1.1 2,636,418 + Psyr_2270 0.73 -0.6 2,636,418 + Psyr_2270 0.73 +1.1 2,636,419 - Psyr_2270 0.73 -1.8 2,636,419 - Psyr_2270 0.73 +2.6 2,636,419 - Psyr_2270 0.73 +0.5 2,636,419 - Psyr_2270 0.73 +0.4 2,636,419 - Psyr_2270 0.73 +0.6 2,636,419 - Psyr_2270 0.73 +0.7 2,636,598 + -0.5 2,636,600 + -0.4 2,636,600 + -0.2 2,636,600 + -1.4 2,636,601 - -0.8 2,636,601 - -0.0 2,636,601 - -1.3 2,636,613 - +0.7 2,636,668 + -1.8 2,636,669 - +0.3 2,636,669 - -1.0 2,636,782 + -1.8 2,636,782 + +0.4 2,636,963 + Psyr_2271 0.22 -0.5 2,636,964 - Psyr_2271 0.22 +4.3
Or see this region's nucleotide sequence