Experiment: H2 McCas with 40 minute oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02935 and MMP_RS02940 overlap by 4 nucleotides MMP_RS02940 and MMP_RS02945 are separated by 1 nucleotides MMP_RS02945 and MMP_RS02950 are separated by 9 nucleotides MMP_RS02950 and MMP_RS02955 are separated by 118 nucleotides
MMP_RS02935: MMP_RS02935 - A24 family peptidase, at 558,325 to 559,017
_RS02935
MMP_RS02940: MMP_RS02940 - TatD family hydrolase, at 559,014 to 559,856
_RS02940
MMP_RS02945: MMP_RS02945 - UPF0146 family protein, at 559,858 to 560,271
_RS02945
MMP_RS02950: MMP_RS02950 - hypothetical protein, at 560,281 to 560,577
_RS02950
MMP_RS02955: MMP_RS02955 - DJ-1/PfpI family protein, at 560,696 to 561,211
_RS02955
Position (kb) 559
560
561 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 558.962 kb on + strand at 558.962 kb on + strand at 558.965 kb on - strand at 558.966 kb on + strand at 558.966 kb on + strand at 558.969 kb on + strand at 558.992 kb on - strand at 559.221 kb on - strand, within MMP_RS02940 at 559.272 kb on - strand, within MMP_RS02940 at 559.324 kb on + strand, within MMP_RS02940 at 559.426 kb on - strand, within MMP_RS02940 at 559.554 kb on - strand, within MMP_RS02940 at 559.555 kb on + strand, within MMP_RS02940 at 559.555 kb on + strand, within MMP_RS02940 at 559.562 kb on + strand, within MMP_RS02940 at 559.735 kb on + strand, within MMP_RS02940 at 559.877 kb on - strand at 560.125 kb on - strand at 560.131 kb on - strand at 560.250 kb on - strand at 560.252 kb on - strand at 560.253 kb on + strand at 560.282 kb on - strand at 560.604 kb on - strand at 560.616 kb on - strand at 560.627 kb on - strand at 560.636 kb on - strand at 560.647 kb on + strand at 560.651 kb on - strand at 560.659 kb on + strand at 560.659 kb on + strand at 560.659 kb on + strand at 560.660 kb on - strand at 560.660 kb on - strand at 560.660 kb on - strand at 560.660 kb on - strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.707 kb on + strand at 560.757 kb on - strand, within MMP_RS02955 at 560.764 kb on + strand, within MMP_RS02955 at 560.896 kb on - strand, within MMP_RS02955 at 560.897 kb on + strand, within MMP_RS02955 at 561.250 kb on - strand at 561.251 kb on + strand at 561.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 40 minute oxygen exposure remove 558,962 + -2.4 558,962 + -1.8 558,965 - -2.1 558,966 + -2.6 558,966 + -3.0 558,969 + -1.7 558,992 - -2.1 559,221 - MMP_RS02940 0.25 -0.1 559,272 - MMP_RS02940 0.31 +0.6 559,324 + MMP_RS02940 0.37 -0.4 559,426 - MMP_RS02940 0.49 -0.3 559,554 - MMP_RS02940 0.64 -0.3 559,555 + MMP_RS02940 0.64 +0.0 559,555 + MMP_RS02940 0.64 +0.4 559,562 + MMP_RS02940 0.65 -0.7 559,735 + MMP_RS02940 0.86 -0.3 559,877 - -2.8 560,125 - -2.3 560,131 - -2.5 560,250 - -1.9 560,252 - -1.9 560,253 + -1.5 560,282 - +0.2 560,604 - +0.2 560,616 - -0.8 560,627 - -0.1 560,636 - +0.0 560,647 + +0.7 560,651 - -0.8 560,659 + -1.2 560,659 + +0.4 560,659 + -0.9 560,660 - +0.5 560,660 - +0.2 560,660 - +0.3 560,660 - -1.4 560,661 + +0.2 560,661 + -0.1 560,661 + +0.2 560,661 + -0.2 560,661 + -1.3 560,661 + +0.5 560,707 + -0.4 560,757 - MMP_RS02955 0.12 -0.6 560,764 + MMP_RS02955 0.13 +0.0 560,896 - MMP_RS02955 0.39 -4.5 560,897 + MMP_RS02955 0.39 -0.2 561,250 - +0.5 561,251 + -1.2 561,251 + +0.5
Or see this region's nucleotide sequence