Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_06235

Experiment: Bu1_100

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_06234 and H281DRAFT_06235 are separated by 17 nucleotidesH281DRAFT_06235 and H281DRAFT_06236 are separated by 23 nucleotides H281DRAFT_06234: H281DRAFT_06234 - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase, at 13,649 to 15,076 _06234 H281DRAFT_06235: H281DRAFT_06235 - Phospho-N-acetylmuramoyl-pentapeptide-transferase, at 15,094 to 16,263 _06235 H281DRAFT_06236: H281DRAFT_06236 - UDP-N-acetylmuramoylalanine--D-glutamate ligase, at 16,287 to 17,801 _06236 Position (kb) 15 16 17Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 15.079 kb on + strandat 15.079 kb on + strandat 15.079 kb on + strandat 15.079 kb on + strandat 16.269 kb on + strandat 16.269 kb on + strandat 16.272 kb on + strandat 16.272 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bu1_100
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15,079 + +0.9
15,079 + +1.2
15,079 + -1.2
15,079 + +1.4
16,269 + -0.5
16,269 + -3.8
16,272 + -1.1
16,272 + -0.8

Or see this region's nucleotide sequence