Experiment: Bu1_100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04117 and H281DRAFT_04118 are separated by 183 nucleotides H281DRAFT_04118 and H281DRAFT_04119 are separated by 447 nucleotides H281DRAFT_04119 and H281DRAFT_04120 are separated by 269 nucleotides
H281DRAFT_04117: H281DRAFT_04117 - SSU ribosomal protein S20P, at 228,555 to 228,833
_04117
H281DRAFT_04118: H281DRAFT_04118 - Protein of unknown function (DUF3579), at 229,017 to 229,364
_04118
H281DRAFT_04119: H281DRAFT_04119 - ornithine carbamoyltransferase, at 229,812 to 230,741
_04119
H281DRAFT_04120: H281DRAFT_04120 - argininosuccinate synthase, at 231,011 to 232,237
_04120
Position (kb) 229
230
231 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 228.859 kb on + strand at 228.860 kb on - strand at 228.924 kb on + strand at 229.373 kb on - strand at 229.556 kb on - strand at 229.619 kb on - strand at 229.750 kb on - strand at 229.756 kb on + strand at 229.757 kb on - strand at 229.802 kb on + strand at 229.802 kb on + strand at 229.802 kb on + strand at 229.836 kb on + strand at 229.837 kb on - strand at 229.861 kb on + strand at 229.862 kb on - strand at 229.869 kb on + strand at 229.870 kb on - strand at 229.870 kb on - strand at 229.875 kb on + strand at 229.875 kb on + strand at 229.920 kb on + strand, within H281DRAFT_04119 at 229.929 kb on + strand, within H281DRAFT_04119 at 229.929 kb on + strand, within H281DRAFT_04119 at 229.929 kb on + strand, within H281DRAFT_04119 at 229.929 kb on + strand at 229.930 kb on - strand, within H281DRAFT_04119 at 229.930 kb on - strand, within H281DRAFT_04119 at 229.930 kb on - strand, within H281DRAFT_04119 at 229.930 kb on - strand, within H281DRAFT_04119 at 230.150 kb on + strand, within H281DRAFT_04119 at 230.150 kb on + strand, within H281DRAFT_04119 at 230.304 kb on - strand, within H281DRAFT_04119 at 230.663 kb on - strand at 230.663 kb on - strand at 230.713 kb on - strand at 230.941 kb on + strand at 230.941 kb on + strand at 231.000 kb on - strand at 231.000 kb on - strand at 231.042 kb on - strand at 231.092 kb on + strand at 231.092 kb on + strand at 231.093 kb on - strand at 231.093 kb on - strand at 231.266 kb on + strand, within H281DRAFT_04120 at 231.267 kb on - strand, within H281DRAFT_04120 at 231.278 kb on + strand, within H281DRAFT_04120 at 231.278 kb on + strand, within H281DRAFT_04120 at 231.413 kb on + strand, within H281DRAFT_04120 at 231.413 kb on + strand at 231.414 kb on - strand, within H281DRAFT_04120 at 231.416 kb on + strand, within H281DRAFT_04120 at 231.495 kb on - strand, within H281DRAFT_04120 at 231.680 kb on + strand, within H281DRAFT_04120 at 231.680 kb on + strand, within H281DRAFT_04120 at 231.680 kb on + strand, within H281DRAFT_04120 at 231.681 kb on - strand, within H281DRAFT_04120
Per-strain Table
Position Strand Gene LocusTag Fraction Bu1_100 remove 228,859 + -1.9 228,860 - +0.7 228,924 + -0.1 229,373 - -1.7 229,556 - -0.1 229,619 - +0.9 229,750 - -2.1 229,756 + -1.3 229,757 - -2.1 229,802 + +2.0 229,802 + -1.0 229,802 + -0.4 229,836 + -1.7 229,837 - -1.7 229,861 + -0.1 229,862 - -2.4 229,869 + -2.7 229,870 - -0.7 229,870 - -1.4 229,875 + -0.6 229,875 + -3.7 229,920 + H281DRAFT_04119 0.12 -1.1 229,929 + H281DRAFT_04119 0.13 -3.1 229,929 + H281DRAFT_04119 0.13 -3.7 229,929 + H281DRAFT_04119 0.13 -3.7 229,929 + -0.8 229,930 - H281DRAFT_04119 0.13 -2.6 229,930 - H281DRAFT_04119 0.13 +1.0 229,930 - H281DRAFT_04119 0.13 -1.1 229,930 - H281DRAFT_04119 0.13 -2.4 230,150 + H281DRAFT_04119 0.36 -0.8 230,150 + H281DRAFT_04119 0.36 -4.0 230,304 - H281DRAFT_04119 0.53 -4.0 230,663 - -1.1 230,663 - -3.1 230,713 - -1.9 230,941 + +1.8 230,941 + -3.4 231,000 - -2.5 231,000 - -0.6 231,042 - -1.1 231,092 + -0.2 231,092 + -0.4 231,093 - -1.8 231,093 - -2.6 231,266 + H281DRAFT_04120 0.21 -2.9 231,267 - H281DRAFT_04120 0.21 -2.6 231,278 + H281DRAFT_04120 0.22 +2.0 231,278 + H281DRAFT_04120 0.22 -0.3 231,413 + H281DRAFT_04120 0.33 -1.3 231,413 + +1.0 231,414 - H281DRAFT_04120 0.33 -2.7 231,416 + H281DRAFT_04120 0.33 +0.0 231,495 - H281DRAFT_04120 0.39 +0.4 231,680 + H281DRAFT_04120 0.55 -2.3 231,680 + H281DRAFT_04120 0.55 -2.6 231,680 + H281DRAFT_04120 0.55 -3.4 231,681 - H281DRAFT_04120 0.55 -0.9
Or see this region's nucleotide sequence