Experiment: Bu1_100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01350 and H281DRAFT_01351 are separated by 351 nucleotides H281DRAFT_01351 and H281DRAFT_01352 are separated by 200 nucleotides H281DRAFT_01352 and H281DRAFT_01353 are separated by 57 nucleotides
H281DRAFT_01350: H281DRAFT_01350 - DNA-binding protein HU-beta, at 193,193 to 193,471
_01350
H281DRAFT_01351: H281DRAFT_01351 - hypothetical protein, at 193,823 to 194,206
_01351
H281DRAFT_01352: H281DRAFT_01352 - diguanylate cyclase/phosphodiesterase, at 194,407 to 196,380
_01352
H281DRAFT_01353: H281DRAFT_01353 - Predicted dithiol-disulfide oxidoreductase, DUF899 family, at 196,438 to 197,166
_01353
Position (kb)
194
195
196
197 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 193.488 kb on + strand at 193.489 kb on - strand at 193.506 kb on + strand at 193.541 kb on + strand at 193.542 kb on - strand at 193.579 kb on + strand at 193.579 kb on + strand at 193.580 kb on - strand at 193.642 kb on + strand at 193.642 kb on + strand at 193.642 kb on + strand at 193.643 kb on - strand at 193.839 kb on + strand at 193.839 kb on + strand at 193.839 kb on + strand at 193.839 kb on + strand at 193.840 kb on - strand at 193.840 kb on - strand at 194.116 kb on - strand, within H281DRAFT_01351 at 194.134 kb on + strand, within H281DRAFT_01351 at 194.184 kb on + strand at 194.184 kb on + strand at 194.260 kb on - strand at 194.276 kb on + strand at 194.355 kb on + strand at 194.355 kb on + strand at 194.356 kb on - strand at 194.367 kb on + strand at 194.513 kb on - strand at 194.630 kb on + strand, within H281DRAFT_01352 at 194.794 kb on + strand, within H281DRAFT_01352 at 195.429 kb on + strand, within H281DRAFT_01352 at 195.430 kb on - strand, within H281DRAFT_01352 at 195.523 kb on + strand, within H281DRAFT_01352 at 195.810 kb on - strand, within H281DRAFT_01352 at 195.810 kb on - strand, within H281DRAFT_01352 at 196.085 kb on - strand at 196.288 kb on + strand at 196.439 kb on + strand at 196.628 kb on - strand, within H281DRAFT_01353 at 196.689 kb on + strand, within H281DRAFT_01353 at 196.689 kb on + strand, within H281DRAFT_01353 at 196.707 kb on - strand, within H281DRAFT_01353 at 196.842 kb on + strand, within H281DRAFT_01353 at 196.889 kb on + strand, within H281DRAFT_01353 at 196.890 kb on - strand, within H281DRAFT_01353 at 197.039 kb on - strand, within H281DRAFT_01353
Per-strain Table
Position Strand Gene LocusTag Fraction Bu1_100 remove 193,488 + -3.8 193,489 - -0.2 193,506 + -0.2 193,541 + -2.7 193,542 - +0.6 193,579 + +0.6 193,579 + +1.4 193,580 - -1.8 193,642 + -0.2 193,642 + +0.5 193,642 + -1.8 193,643 - -0.7 193,839 + +1.6 193,839 + +2.0 193,839 + +0.1 193,839 + -0.7 193,840 - +0.6 193,840 - -2.1 194,116 - H281DRAFT_01351 0.76 +0.6 194,134 + H281DRAFT_01351 0.81 -2.3 194,184 + -0.9 194,184 + +0.2 194,260 - -0.3 194,276 + +0.6 194,355 + +1.2 194,355 + +0.7 194,356 - -0.2 194,367 + +0.4 194,513 - -0.0 194,630 + H281DRAFT_01352 0.11 +0.2 194,794 + H281DRAFT_01352 0.20 -0.3 195,429 + H281DRAFT_01352 0.52 -0.4 195,430 - H281DRAFT_01352 0.52 +1.1 195,523 + H281DRAFT_01352 0.57 -0.1 195,810 - H281DRAFT_01352 0.71 +1.2 195,810 - H281DRAFT_01352 0.71 +1.1 196,085 - +0.5 196,288 + +0.5 196,439 + +0.5 196,628 - H281DRAFT_01353 0.26 -1.4 196,689 + H281DRAFT_01353 0.34 -0.3 196,689 + H281DRAFT_01353 0.34 +1.1 196,707 - H281DRAFT_01353 0.37 +1.1 196,842 + H281DRAFT_01353 0.55 +2.1 196,889 + H281DRAFT_01353 0.62 +1.1 196,890 - H281DRAFT_01353 0.62 +0.7 197,039 - H281DRAFT_01353 0.82 -0.7
Or see this region's nucleotide sequence