Experiment: LB in EcoFAB_10mL EcoFab with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20790 and BWI76_RS20795 are separated by 11 nucleotides BWI76_RS20795 and BWI76_RS20800 are separated by 114 nucleotides BWI76_RS20800 and BWI76_RS20805 are separated by 36 nucleotides BWI76_RS20805 and BWI76_RS20810 overlap by 4 nucleotides BWI76_RS20810 and BWI76_RS20815 overlap by 4 nucleotides
BWI76_RS20790: BWI76_RS20790 - aldehyde dehydrogenase, at 4,345,900 to 4,347,303
_RS20790
BWI76_RS20795: BWI76_RS20795 - ethanolamine catabolic microcompartment shell protein EutN, at 4,347,315 to 4,347,602
_RS20795
BWI76_RS20800: BWI76_RS20800 - carboxysome shell protein, at 4,347,717 to 4,348,007
_RS20800
BWI76_RS20805: BWI76_RS20805 - phosphate acetyltransferase, at 4,348,044 to 4,349,060
_RS20805
BWI76_RS20810: BWI76_RS20810 - ethanolamine utilization cobalamin adenosyltransferase, at 4,349,057 to 4,349,860
_RS20810
BWI76_RS20815: BWI76_RS20815 - ethanolamine utilization protein EutQ, at 4,349,857 to 4,350,549
_RS20815
Position (kb) 4348
4349
4350 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4347.523 kb on + strand, within BWI76_RS20795 at 4347.955 kb on + strand, within BWI76_RS20800 at 4347.963 kb on - strand, within BWI76_RS20800 at 4347.963 kb on - strand, within BWI76_RS20800 at 4348.572 kb on + strand, within BWI76_RS20805 at 4348.572 kb on + strand, within BWI76_RS20805 at 4348.572 kb on + strand, within BWI76_RS20805 at 4348.910 kb on + strand, within BWI76_RS20805 at 4349.142 kb on + strand, within BWI76_RS20810 at 4349.150 kb on - strand, within BWI76_RS20810 at 4349.150 kb on - strand, within BWI76_RS20810 at 4349.150 kb on - strand, within BWI76_RS20810 at 4349.150 kb on - strand, within BWI76_RS20810 at 4349.196 kb on - strand, within BWI76_RS20810 at 4349.340 kb on + strand, within BWI76_RS20810 at 4349.958 kb on - strand, within BWI76_RS20815 at 4349.963 kb on + strand, within BWI76_RS20815 at 4350.030 kb on + strand, within BWI76_RS20815 at 4350.040 kb on - strand, within BWI76_RS20815
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL EcoFab with sand remove 4,347,523 + BWI76_RS20795 0.72 -1.2 4,347,955 + BWI76_RS20800 0.82 +0.4 4,347,963 - BWI76_RS20800 0.85 -0.0 4,347,963 - BWI76_RS20800 0.85 -0.6 4,348,572 + BWI76_RS20805 0.52 -0.0 4,348,572 + BWI76_RS20805 0.52 +0.9 4,348,572 + BWI76_RS20805 0.52 +1.5 4,348,910 + BWI76_RS20805 0.85 +2.0 4,349,142 + BWI76_RS20810 0.11 -0.2 4,349,150 - BWI76_RS20810 0.12 +0.7 4,349,150 - BWI76_RS20810 0.12 -0.4 4,349,150 - BWI76_RS20810 0.12 -2.4 4,349,150 - BWI76_RS20810 0.12 -0.3 4,349,196 - BWI76_RS20810 0.17 -0.1 4,349,340 + BWI76_RS20810 0.35 +0.1 4,349,958 - BWI76_RS20815 0.15 -0.3 4,349,963 + BWI76_RS20815 0.15 -0.2 4,350,030 + BWI76_RS20815 0.25 -1.1 4,350,040 - BWI76_RS20815 0.26 +0.2
Or see this region's nucleotide sequence