Experiment: LB in EcoFAB_10mL EcoFab with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14420 and BWI76_RS14425 are separated by 77 nucleotides BWI76_RS14425 and BWI76_RS14430 are separated by 287 nucleotides BWI76_RS14430 and BWI76_RS14435 are separated by 19 nucleotides BWI76_RS14435 and BWI76_RS14440 are separated by 2 nucleotides
BWI76_RS14420: BWI76_RS14420 - hypothetical protein, at 3,010,700 to 3,011,482
_RS14420
BWI76_RS14425: BWI76_RS14425 - hypothetical protein, at 3,011,560 to 3,011,739
_RS14425
BWI76_RS14430: BWI76_RS14430 - peptidase C45, at 3,012,027 to 3,013,049
_RS14430
BWI76_RS14435: BWI76_RS14435 - glutamine ABC transporter ATP-binding protein, at 3,013,069 to 3,013,791
_RS14435
BWI76_RS14440: BWI76_RS14440 - glutamine ABC transporter permease GlnP, at 3,013,794 to 3,014,450
_RS14440
Position (kb)
3012
3013
3014 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3011.031 kb on - strand, within BWI76_RS14420 at 3011.036 kb on - strand, within BWI76_RS14420 at 3011.038 kb on - strand, within BWI76_RS14420 at 3011.048 kb on - strand, within BWI76_RS14420 at 3011.053 kb on - strand, within BWI76_RS14420 at 3011.077 kb on - strand, within BWI76_RS14420 at 3011.149 kb on + strand, within BWI76_RS14420 at 3011.172 kb on + strand, within BWI76_RS14420 at 3011.180 kb on - strand, within BWI76_RS14420 at 3011.180 kb on - strand, within BWI76_RS14420 at 3011.180 kb on - strand, within BWI76_RS14420 at 3011.270 kb on + strand, within BWI76_RS14420 at 3011.278 kb on - strand, within BWI76_RS14420 at 3011.289 kb on - strand, within BWI76_RS14420 at 3011.294 kb on - strand, within BWI76_RS14420 at 3011.398 kb on - strand, within BWI76_RS14420 at 3011.691 kb on - strand, within BWI76_RS14425 at 3011.703 kb on - strand, within BWI76_RS14425 at 3011.729 kb on - strand at 3011.754 kb on - strand at 3011.762 kb on - strand at 3011.775 kb on + strand at 3011.781 kb on - strand at 3011.852 kb on + strand at 3011.860 kb on - strand at 3011.873 kb on + strand at 3011.876 kb on - strand at 3011.898 kb on + strand at 3011.898 kb on + strand at 3011.898 kb on + strand at 3011.898 kb on + strand at 3011.928 kb on - strand at 3011.966 kb on + strand at 3011.979 kb on - strand at 3011.990 kb on + strand at 3011.990 kb on + strand at 3011.990 kb on + strand at 3011.998 kb on - strand at 3012.116 kb on - strand at 3012.239 kb on - strand, within BWI76_RS14430 at 3012.280 kb on - strand, within BWI76_RS14430 at 3012.362 kb on + strand, within BWI76_RS14430 at 3012.372 kb on + strand, within BWI76_RS14430 at 3012.380 kb on - strand, within BWI76_RS14430 at 3012.386 kb on + strand, within BWI76_RS14430 at 3012.420 kb on + strand, within BWI76_RS14430 at 3012.659 kb on + strand, within BWI76_RS14430 at 3013.010 kb on - strand at 3013.134 kb on - strand at 3013.181 kb on - strand, within BWI76_RS14435 at 3013.705 kb on - strand, within BWI76_RS14435 at 3013.746 kb on - strand at 3013.847 kb on + strand at 3013.849 kb on - strand at 3013.879 kb on + strand, within BWI76_RS14440 at 3013.879 kb on + strand, within BWI76_RS14440 at 3013.882 kb on - strand, within BWI76_RS14440 at 3013.882 kb on - strand, within BWI76_RS14440 at 3013.887 kb on - strand, within BWI76_RS14440 at 3013.887 kb on - strand, within BWI76_RS14440 at 3013.887 kb on - strand, within BWI76_RS14440 at 3013.887 kb on - strand, within BWI76_RS14440 at 3013.894 kb on - strand, within BWI76_RS14440 at 3013.962 kb on - strand, within BWI76_RS14440 at 3013.967 kb on - strand, within BWI76_RS14440 at 3013.981 kb on - strand, within BWI76_RS14440 at 3014.019 kb on + strand, within BWI76_RS14440 at 3014.019 kb on + strand, within BWI76_RS14440 at 3014.019 kb on + strand, within BWI76_RS14440 at 3014.041 kb on - strand, within BWI76_RS14440 at 3014.046 kb on + strand, within BWI76_RS14440
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL EcoFab with sand remove 3,011,031 - BWI76_RS14420 0.42 -1.1 3,011,036 - BWI76_RS14420 0.43 +0.4 3,011,038 - BWI76_RS14420 0.43 -0.5 3,011,048 - BWI76_RS14420 0.44 -0.5 3,011,053 - BWI76_RS14420 0.45 +0.2 3,011,077 - BWI76_RS14420 0.48 -0.5 3,011,149 + BWI76_RS14420 0.57 +0.4 3,011,172 + BWI76_RS14420 0.60 -0.7 3,011,180 - BWI76_RS14420 0.61 +0.5 3,011,180 - BWI76_RS14420 0.61 -0.4 3,011,180 - BWI76_RS14420 0.61 +0.1 3,011,270 + BWI76_RS14420 0.73 +0.3 3,011,278 - BWI76_RS14420 0.74 +0.3 3,011,289 - BWI76_RS14420 0.75 -0.9 3,011,294 - BWI76_RS14420 0.76 +1.3 3,011,398 - BWI76_RS14420 0.89 +1.6 3,011,691 - BWI76_RS14425 0.73 -0.7 3,011,703 - BWI76_RS14425 0.79 -1.1 3,011,729 - -0.3 3,011,754 - -1.4 3,011,762 - -0.7 3,011,775 + -2.0 3,011,781 - -0.6 3,011,852 + +0.8 3,011,860 - -0.2 3,011,873 + -0.0 3,011,876 - -1.2 3,011,898 + +1.2 3,011,898 + -0.7 3,011,898 + +0.6 3,011,898 + -1.1 3,011,928 - -0.3 3,011,966 + -0.4 3,011,979 - -0.6 3,011,990 + +0.1 3,011,990 + -0.9 3,011,990 + -0.4 3,011,998 - -0.6 3,012,116 - -1.8 3,012,239 - BWI76_RS14430 0.21 +1.1 3,012,280 - BWI76_RS14430 0.25 -0.4 3,012,362 + BWI76_RS14430 0.33 -0.2 3,012,372 + BWI76_RS14430 0.34 -0.5 3,012,380 - BWI76_RS14430 0.35 +0.3 3,012,386 + BWI76_RS14430 0.35 -0.0 3,012,420 + BWI76_RS14430 0.38 +0.2 3,012,659 + BWI76_RS14430 0.62 -1.3 3,013,010 - +0.4 3,013,134 - +0.0 3,013,181 - BWI76_RS14435 0.15 +0.0 3,013,705 - BWI76_RS14435 0.88 -2.3 3,013,746 - -0.9 3,013,847 + +0.1 3,013,849 - -0.1 3,013,879 + BWI76_RS14440 0.13 -0.0 3,013,879 + BWI76_RS14440 0.13 -0.3 3,013,882 - BWI76_RS14440 0.13 +0.0 3,013,882 - BWI76_RS14440 0.13 -0.6 3,013,887 - BWI76_RS14440 0.14 -0.8 3,013,887 - BWI76_RS14440 0.14 +0.8 3,013,887 - BWI76_RS14440 0.14 +0.0 3,013,887 - BWI76_RS14440 0.14 +1.2 3,013,894 - BWI76_RS14440 0.15 -0.2 3,013,962 - BWI76_RS14440 0.26 +0.4 3,013,967 - BWI76_RS14440 0.26 -1.7 3,013,981 - BWI76_RS14440 0.28 -0.4 3,014,019 + BWI76_RS14440 0.34 -0.7 3,014,019 + BWI76_RS14440 0.34 -0.3 3,014,019 + BWI76_RS14440 0.34 -2.8 3,014,041 - BWI76_RS14440 0.38 +0.1 3,014,046 + BWI76_RS14440 0.38 +1.6
Or see this region's nucleotide sequence