Experiment: LB in EcoFAB_10mL EcoFab with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08520 and BWI76_RS08525 are separated by 3 nucleotides BWI76_RS08525 and BWI76_RS08530 are separated by 105 nucleotides BWI76_RS08530 and BWI76_RS08535 are separated by 128 nucleotides
BWI76_RS08520: BWI76_RS08520 - colicin transporter TolQ, at 1,803,797 to 1,804,480
_RS08520
BWI76_RS08525: BWI76_RS08525 - colicin transporter, at 1,804,484 to 1,804,912
_RS08525
BWI76_RS08530: BWI76_RS08530 - protein TolA, at 1,805,018 to 1,806,355
_RS08530
BWI76_RS08535: BWI76_RS08535 - translocation protein TolB, at 1,806,484 to 1,807,776
_RS08535
Position (kb) 1805
1806
1807 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1804.358 kb on + strand, within BWI76_RS08520 at 1804.368 kb on + strand, within BWI76_RS08520 at 1804.421 kb on - strand at 1804.433 kb on + strand at 1804.486 kb on + strand at 1804.491 kb on + strand at 1804.546 kb on + strand, within BWI76_RS08525 at 1804.697 kb on + strand, within BWI76_RS08525 at 1804.753 kb on + strand, within BWI76_RS08525 at 1805.247 kb on + strand, within BWI76_RS08530 at 1806.168 kb on - strand, within BWI76_RS08530 at 1806.468 kb on + strand at 1806.476 kb on - strand at 1806.619 kb on + strand, within BWI76_RS08535 at 1806.763 kb on - strand at 1807.007 kb on - strand, within BWI76_RS08535 at 1807.291 kb on - strand, within BWI76_RS08535
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL EcoFab with sand remove 1,804,358 + BWI76_RS08520 0.82 -1.3 1,804,368 + BWI76_RS08520 0.83 -3.0 1,804,421 - -1.0 1,804,433 + -2.5 1,804,486 + -3.8 1,804,491 + -2.6 1,804,546 + BWI76_RS08525 0.14 -3.1 1,804,697 + BWI76_RS08525 0.50 +1.4 1,804,753 + BWI76_RS08525 0.63 -1.3 1,805,247 + BWI76_RS08530 0.17 -3.8 1,806,168 - BWI76_RS08530 0.86 -5.1 1,806,468 + +0.4 1,806,476 - -1.7 1,806,619 + BWI76_RS08535 0.10 -3.7 1,806,763 - +1.0 1,807,007 - BWI76_RS08535 0.40 -2.1 1,807,291 - BWI76_RS08535 0.62 -0.2
Or see this region's nucleotide sequence