Experiment: LB in EcoFAB_10mL EcoFab with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04345 and BWI76_RS04350 are separated by 54 nucleotides BWI76_RS04350 and BWI76_RS04355 are separated by 1 nucleotides BWI76_RS04355 and BWI76_RS04360 are separated by 65 nucleotides
BWI76_RS04345: BWI76_RS04345 - signal peptidase II, at 895,864 to 896,364
_RS04345
BWI76_RS04350: BWI76_RS04350 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 896,419 to 896,868
_RS04350
BWI76_RS04355: BWI76_RS04355 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 896,870 to 897,820
_RS04355
BWI76_RS04360: BWI76_RS04360 - ribonucleoside hydrolase RihC, at 897,886 to 898,800
_RS04360
Position (kb) 896
897
898 Strain fitness (log2 ratio) -1
0
1 at 896.421 kb on + strand at 896.558 kb on + strand, within BWI76_RS04350 at 897.853 kb on + strand at 897.853 kb on + strand at 898.063 kb on - strand, within BWI76_RS04360 at 898.115 kb on - strand, within BWI76_RS04360 at 898.350 kb on + strand, within BWI76_RS04360 at 898.453 kb on - strand at 898.558 kb on + strand, within BWI76_RS04360 at 898.628 kb on + strand, within BWI76_RS04360
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL EcoFab with sand remove 896,421 + -0.3 896,558 + BWI76_RS04350 0.31 -0.6 897,853 + +0.1 897,853 + +0.5 898,063 - BWI76_RS04360 0.19 +1.5 898,115 - BWI76_RS04360 0.25 +0.1 898,350 + BWI76_RS04360 0.51 +0.9 898,453 - +0.2 898,558 + BWI76_RS04360 0.73 +0.6 898,628 + BWI76_RS04360 0.81 +0.1
Or see this region's nucleotide sequence