Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mntB and mntA are separated by 0 nucleotides mntA and mntC are separated by 60 nucleotides mntC and 5S ribosomal RNA are separated by 130 nucleotides 5S ribosomal RNA and 23S ribosomal RNA are separated by 70 nucleotides
Synpcc7942_2573: mntB - manganese transport system membrane protein MntB, at 2,652,516 to 2,653,346
mntB
Synpcc7942_2574: mntA - ATPase, at 2,653,347 to 2,654,102
mntA
Synpcc7942_2575: mntC - Mn transporter MntC, at 2,654,163 to 2,655,104
mntC
Synpcc7942_R0050: 5S ribosomal RNA - 5S ribosomal RNA, at 2,655,235 to 2,655,349
5S ribosomal RNA
Synpcc7942_R0051: 23S ribosomal RNA - 23S ribosomal RNA, at 2,655,420 to 2,658,297
23S ribosomal RNA
Position (kb)
2654
2655
2656 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2653.284 kb on - strand at 2653.341 kb on - strand at 2653.343 kb on + strand at 2653.384 kb on + strand at 2653.384 kb on + strand at 2653.384 kb on + strand at 2653.389 kb on + strand at 2653.392 kb on - strand at 2653.458 kb on - strand, within mntA at 2653.671 kb on - strand, within mntA at 2653.716 kb on + strand, within mntA at 2653.733 kb on - strand, within mntA at 2653.734 kb on - strand, within mntA at 2653.884 kb on + strand, within mntA at 2653.924 kb on + strand, within mntA at 2653.927 kb on - strand, within mntA at 2653.994 kb on - strand, within mntA at 2654.015 kb on - strand, within mntA at 2654.050 kb on - strand at 2654.105 kb on + strand at 2654.203 kb on + strand at 2654.217 kb on - strand at 2654.263 kb on + strand, within mntC at 2654.293 kb on + strand, within mntC at 2654.308 kb on + strand, within mntC at 2654.388 kb on + strand, within mntC at 2654.396 kb on - strand, within mntC at 2654.396 kb on - strand, within mntC at 2654.398 kb on + strand, within mntC at 2654.414 kb on + strand, within mntC at 2654.484 kb on + strand, within mntC at 2654.602 kb on - strand, within mntC at 2654.726 kb on + strand, within mntC at 2654.755 kb on - strand, within mntC at 2654.757 kb on - strand, within mntC at 2654.771 kb on + strand, within mntC at 2654.777 kb on + strand, within mntC at 2654.853 kb on + strand, within mntC at 2654.861 kb on - strand, within mntC at 2654.867 kb on - strand, within mntC at 2654.978 kb on + strand, within mntC at 2654.978 kb on + strand, within mntC at 2654.991 kb on - strand, within mntC at 2655.023 kb on - strand at 2655.076 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 2,653,284 - +0.1 2,653,341 - -1.0 2,653,343 + -1.5 2,653,384 + -1.2 2,653,384 + +1.0 2,653,384 + +0.1 2,653,389 + +0.4 2,653,392 - +2.0 2,653,458 - mntA Synpcc7942_2574 0.15 +2.6 2,653,671 - mntA Synpcc7942_2574 0.43 -0.3 2,653,716 + mntA Synpcc7942_2574 0.49 -0.0 2,653,733 - mntA Synpcc7942_2574 0.51 +2.0 2,653,734 - mntA Synpcc7942_2574 0.51 -0.0 2,653,884 + mntA Synpcc7942_2574 0.71 -0.2 2,653,924 + mntA Synpcc7942_2574 0.76 +0.4 2,653,927 - mntA Synpcc7942_2574 0.77 +0.2 2,653,994 - mntA Synpcc7942_2574 0.86 -0.6 2,654,015 - mntA Synpcc7942_2574 0.88 -0.0 2,654,050 - -0.2 2,654,105 + -1.3 2,654,203 + -1.0 2,654,217 - -0.0 2,654,263 + mntC Synpcc7942_2575 0.11 +2.6 2,654,293 + mntC Synpcc7942_2575 0.14 +0.5 2,654,308 + mntC Synpcc7942_2575 0.15 -0.6 2,654,388 + mntC Synpcc7942_2575 0.24 -3.1 2,654,396 - mntC Synpcc7942_2575 0.25 +2.0 2,654,396 - mntC Synpcc7942_2575 0.25 -2.0 2,654,398 + mntC Synpcc7942_2575 0.25 -0.3 2,654,414 + mntC Synpcc7942_2575 0.27 +1.4 2,654,484 + mntC Synpcc7942_2575 0.34 -1.7 2,654,602 - mntC Synpcc7942_2575 0.47 -0.6 2,654,726 + mntC Synpcc7942_2575 0.60 +1.0 2,654,755 - mntC Synpcc7942_2575 0.63 +1.0 2,654,757 - mntC Synpcc7942_2575 0.63 -1.2 2,654,771 + mntC Synpcc7942_2575 0.65 -2.1 2,654,777 + mntC Synpcc7942_2575 0.65 -1.0 2,654,853 + mntC Synpcc7942_2575 0.73 -0.0 2,654,861 - mntC Synpcc7942_2575 0.74 +2.6 2,654,867 - mntC Synpcc7942_2575 0.75 -0.7 2,654,978 + mntC Synpcc7942_2575 0.87 -1.2 2,654,978 + mntC Synpcc7942_2575 0.87 -0.6 2,654,991 - mntC Synpcc7942_2575 0.88 -1.3 2,655,023 - -2.3 2,655,076 + -0.9
Or see this region's nucleotide sequence