Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt natB and Synpcc7942_1862 are separated by 159 nucleotides Synpcc7942_1862 and hemG are separated by 12 nucleotides hemG and Synpcc7942_1864 are separated by 5 nucleotides
Synpcc7942_1861: natB - periplasmic binding protein of ABC transporter for natural amino acids, at 1,931,340 to 1,932,605
natB
Synpcc7942_1862: Synpcc7942_1862 - hypothetical protein, at 1,932,765 to 1,933,688
_1862
Synpcc7942_1863: hemG - modification methylase, HemK family, at 1,933,701 to 1,934,582
hemG
Synpcc7942_1864: Synpcc7942_1864 - hypothetical protein, at 1,934,588 to 1,935,175
_1864
Position (kb) 1932
1933
1934 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1931.778 kb on - strand, within natB at 1931.788 kb on + strand, within natB at 1931.790 kb on + strand, within natB at 1931.790 kb on + strand, within natB at 1931.796 kb on - strand, within natB at 1931.798 kb on - strand, within natB at 1931.811 kb on + strand, within natB at 1931.825 kb on + strand, within natB at 1931.834 kb on - strand, within natB at 1931.840 kb on - strand, within natB at 1931.887 kb on + strand, within natB at 1931.899 kb on + strand, within natB at 1931.899 kb on + strand, within natB at 1931.932 kb on - strand, within natB at 1931.937 kb on + strand, within natB at 1931.945 kb on - strand, within natB at 1931.949 kb on + strand, within natB at 1931.951 kb on + strand, within natB at 1931.956 kb on + strand, within natB at 1931.959 kb on + strand, within natB at 1931.959 kb on - strand, within natB at 1931.961 kb on + strand, within natB at 1931.964 kb on - strand, within natB at 1931.967 kb on - strand, within natB at 1931.982 kb on + strand, within natB at 1931.990 kb on - strand, within natB at 1932.003 kb on - strand at 1932.005 kb on + strand at 1932.013 kb on - strand, within natB at 1932.035 kb on + strand, within natB at 1932.038 kb on - strand, within natB at 1932.066 kb on + strand, within natB at 1932.074 kb on - strand, within natB at 1932.085 kb on + strand, within natB at 1932.118 kb on - strand, within natB at 1932.118 kb on - strand, within natB at 1932.118 kb on - strand, within natB at 1932.123 kb on - strand, within natB at 1932.128 kb on + strand, within natB at 1932.132 kb on + strand, within natB at 1932.132 kb on + strand, within natB at 1932.191 kb on + strand, within natB at 1932.224 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.247 kb on - strand, within natB at 1932.254 kb on - strand, within natB at 1932.254 kb on - strand, within natB at 1932.255 kb on - strand, within natB at 1932.260 kb on - strand, within natB at 1932.271 kb on - strand, within natB at 1932.298 kb on - strand, within natB at 1932.307 kb on + strand, within natB at 1932.316 kb on + strand, within natB at 1932.329 kb on - strand, within natB at 1932.352 kb on + strand, within natB at 1932.382 kb on + strand, within natB at 1932.387 kb on + strand, within natB at 1932.387 kb on + strand, within natB at 1932.395 kb on - strand, within natB at 1932.395 kb on - strand, within natB at 1932.405 kb on + strand, within natB at 1932.405 kb on + strand, within natB at 1932.405 kb on + strand, within natB at 1932.423 kb on + strand, within natB at 1932.428 kb on + strand, within natB at 1932.428 kb on + strand, within natB at 1932.438 kb on + strand, within natB at 1932.446 kb on - strand, within natB at 1932.446 kb on - strand, within natB at 1932.446 kb on - strand, within natB at 1932.461 kb on - strand, within natB at 1932.496 kb on + strand at 1932.505 kb on - strand at 1932.514 kb on - strand at 1932.549 kb on + strand at 1932.557 kb on + strand at 1932.560 kb on + strand at 1932.560 kb on - strand at 1932.562 kb on + strand at 1932.570 kb on - strand at 1932.580 kb on - strand at 1932.580 kb on - strand at 1932.610 kb on - strand at 1932.611 kb on + strand at 1932.611 kb on + strand at 1932.619 kb on - strand at 1932.628 kb on + strand at 1932.644 kb on + strand at 1932.696 kb on + strand at 1932.696 kb on + strand at 1932.707 kb on + strand at 1932.707 kb on + strand at 1932.715 kb on - strand at 1932.719 kb on + strand at 1932.726 kb on + strand at 1932.727 kb on - strand at 1932.817 kb on - strand at 1932.835 kb on - strand at 1932.892 kb on - strand, within Synpcc7942_1862 at 1932.902 kb on - strand, within Synpcc7942_1862 at 1932.927 kb on - strand, within Synpcc7942_1862 at 1933.126 kb on + strand, within Synpcc7942_1862 at 1933.141 kb on - strand, within Synpcc7942_1862 at 1933.402 kb on - strand, within Synpcc7942_1862 at 1933.449 kb on + strand, within Synpcc7942_1862 at 1933.487 kb on + strand, within Synpcc7942_1862 at 1933.565 kb on + strand, within Synpcc7942_1862 at 1933.657 kb on + strand at 1933.660 kb on - strand at 1933.663 kb on + strand at 1933.663 kb on + strand at 1933.663 kb on + strand at 1933.665 kb on - strand at 1933.671 kb on - strand at 1933.738 kb on - strand at 1933.779 kb on + strand at 1933.801 kb on - strand, within hemG at 1933.824 kb on + strand, within hemG at 1933.837 kb on - strand, within hemG at 1933.842 kb on - strand, within hemG at 1933.852 kb on + strand, within hemG at 1933.857 kb on + strand, within hemG at 1933.857 kb on + strand, within hemG at 1933.862 kb on + strand, within hemG at 1933.870 kb on - strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.947 kb on - strand, within hemG at 1933.991 kb on - strand, within hemG at 1934.039 kb on - strand, within hemG at 1934.044 kb on - strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.084 kb on - strand, within hemG at 1934.084 kb on - strand, within hemG at 1934.146 kb on + strand, within hemG at 1934.178 kb on + strand, within hemG at 1934.183 kb on + strand, within hemG at 1934.191 kb on - strand, within hemG at 1934.225 kb on + strand, within hemG at 1934.226 kb on + strand, within hemG at 1934.229 kb on - strand, within hemG at 1934.234 kb on + strand, within hemG at 1934.240 kb on + strand, within hemG at 1934.260 kb on + strand, within hemG at 1934.330 kb on - strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.352 kb on - strand, within hemG at 1934.352 kb on - strand, within hemG at 1934.362 kb on - strand, within hemG at 1934.374 kb on + strand, within hemG at 1934.391 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.465 kb on - strand, within hemG at 1934.520 kb on - strand at 1934.529 kb on - strand at 1934.567 kb on + strand at 1934.577 kb on + strand at 1934.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 1,931,778 - natB Synpcc7942_1861 0.35 -0.9 1,931,788 + natB Synpcc7942_1861 0.35 -2.5 1,931,790 + natB Synpcc7942_1861 0.36 -1.1 1,931,790 + natB Synpcc7942_1861 0.36 +1.0 1,931,796 - natB Synpcc7942_1861 0.36 -0.9 1,931,798 - natB Synpcc7942_1861 0.36 -1.0 1,931,811 + natB Synpcc7942_1861 0.37 -1.4 1,931,825 + natB Synpcc7942_1861 0.38 -0.8 1,931,834 - natB Synpcc7942_1861 0.39 -1.0 1,931,840 - natB Synpcc7942_1861 0.39 -2.7 1,931,887 + natB Synpcc7942_1861 0.43 +0.6 1,931,899 + natB Synpcc7942_1861 0.44 -0.4 1,931,899 + natB Synpcc7942_1861 0.44 +0.6 1,931,932 - natB Synpcc7942_1861 0.47 +1.5 1,931,937 + natB Synpcc7942_1861 0.47 +1.0 1,931,945 - natB Synpcc7942_1861 0.48 -1.0 1,931,949 + natB Synpcc7942_1861 0.48 +1.0 1,931,951 + natB Synpcc7942_1861 0.48 -0.9 1,931,956 + natB Synpcc7942_1861 0.49 -0.6 1,931,959 + natB Synpcc7942_1861 0.49 -0.0 1,931,959 - natB Synpcc7942_1861 0.49 +1.7 1,931,961 + natB Synpcc7942_1861 0.49 -1.5 1,931,964 - natB Synpcc7942_1861 0.49 -1.8 1,931,967 - natB Synpcc7942_1861 0.50 -1.6 1,931,982 + natB Synpcc7942_1861 0.51 +1.6 1,931,990 - natB Synpcc7942_1861 0.51 -1.0 1,932,003 - -1.5 1,932,005 + -1.8 1,932,013 - natB Synpcc7942_1861 0.53 -2.3 1,932,035 + natB Synpcc7942_1861 0.55 +1.0 1,932,038 - natB Synpcc7942_1861 0.55 -1.8 1,932,066 + natB Synpcc7942_1861 0.57 -0.0 1,932,074 - natB Synpcc7942_1861 0.58 -0.7 1,932,085 + natB Synpcc7942_1861 0.59 -0.6 1,932,118 - natB Synpcc7942_1861 0.61 -2.0 1,932,118 - natB Synpcc7942_1861 0.61 -0.6 1,932,118 - natB Synpcc7942_1861 0.61 -1.2 1,932,123 - natB Synpcc7942_1861 0.62 +0.2 1,932,128 + natB Synpcc7942_1861 0.62 -0.6 1,932,132 + natB Synpcc7942_1861 0.63 -1.0 1,932,132 + natB Synpcc7942_1861 0.63 -0.9 1,932,191 + natB Synpcc7942_1861 0.67 -0.9 1,932,224 + natB Synpcc7942_1861 0.70 -2.3 1,932,231 + natB Synpcc7942_1861 0.70 -2.0 1,932,231 + natB Synpcc7942_1861 0.70 -0.1 1,932,231 + natB Synpcc7942_1861 0.70 -0.8 1,932,231 + natB Synpcc7942_1861 0.70 -0.7 1,932,246 + natB Synpcc7942_1861 0.72 +1.0 1,932,246 + natB Synpcc7942_1861 0.72 -1.5 1,932,246 + natB Synpcc7942_1861 0.72 +1.0 1,932,246 + natB Synpcc7942_1861 0.72 -0.5 1,932,246 + natB Synpcc7942_1861 0.72 +0.3 1,932,246 + natB Synpcc7942_1861 0.72 -1.5 1,932,246 + natB Synpcc7942_1861 0.72 -0.6 1,932,246 + natB Synpcc7942_1861 0.72 +1.0 1,932,247 - natB Synpcc7942_1861 0.72 -1.1 1,932,254 - natB Synpcc7942_1861 0.72 -0.4 1,932,254 - natB Synpcc7942_1861 0.72 -0.1 1,932,255 - natB Synpcc7942_1861 0.72 -0.0 1,932,260 - natB Synpcc7942_1861 0.73 +1.0 1,932,271 - natB Synpcc7942_1861 0.74 +1.2 1,932,298 - natB Synpcc7942_1861 0.76 +2.0 1,932,307 + natB Synpcc7942_1861 0.76 +1.2 1,932,316 + natB Synpcc7942_1861 0.77 -0.7 1,932,329 - natB Synpcc7942_1861 0.78 +2.0 1,932,352 + natB Synpcc7942_1861 0.80 +3.3 1,932,382 + natB Synpcc7942_1861 0.82 -2.1 1,932,387 + natB Synpcc7942_1861 0.83 -1.5 1,932,387 + natB Synpcc7942_1861 0.83 -0.6 1,932,395 - natB Synpcc7942_1861 0.83 -0.3 1,932,395 - natB Synpcc7942_1861 0.83 -0.2 1,932,405 + natB Synpcc7942_1861 0.84 -0.7 1,932,405 + natB Synpcc7942_1861 0.84 -1.4 1,932,405 + natB Synpcc7942_1861 0.84 -0.4 1,932,423 + natB Synpcc7942_1861 0.86 -1.2 1,932,428 + natB Synpcc7942_1861 0.86 -0.1 1,932,428 + natB Synpcc7942_1861 0.86 -1.7 1,932,438 + natB Synpcc7942_1861 0.87 +1.0 1,932,446 - natB Synpcc7942_1861 0.87 -0.8 1,932,446 - natB Synpcc7942_1861 0.87 -0.6 1,932,446 - natB Synpcc7942_1861 0.87 -2.0 1,932,461 - natB Synpcc7942_1861 0.89 -0.4 1,932,496 + +0.3 1,932,505 - -1.1 1,932,514 - +0.1 1,932,549 + -1.5 1,932,557 + +1.6 1,932,560 + +0.9 1,932,560 - +1.0 1,932,562 + -0.7 1,932,570 - -2.3 1,932,580 - -1.5 1,932,580 - +1.0 1,932,610 - +0.4 1,932,611 + +0.5 1,932,611 + -0.8 1,932,619 - -0.4 1,932,628 + -0.4 1,932,644 + +0.1 1,932,696 + +2.0 1,932,696 + +1.4 1,932,707 + +0.7 1,932,707 + -0.0 1,932,715 - +0.5 1,932,719 + -0.3 1,932,726 + -1.0 1,932,727 - +1.0 1,932,817 - +1.0 1,932,835 - +1.0 1,932,892 - Synpcc7942_1862 0.14 -1.0 1,932,902 - Synpcc7942_1862 0.15 -0.0 1,932,927 - Synpcc7942_1862 0.18 -0.0 1,933,126 + Synpcc7942_1862 0.39 +1.0 1,933,141 - Synpcc7942_1862 0.41 -0.6 1,933,402 - Synpcc7942_1862 0.69 +1.0 1,933,449 + Synpcc7942_1862 0.74 -0.0 1,933,487 + Synpcc7942_1862 0.78 +1.0 1,933,565 + Synpcc7942_1862 0.87 +1.0 1,933,657 + -0.5 1,933,660 - -0.7 1,933,663 + -1.4 1,933,663 + -0.0 1,933,663 + -0.8 1,933,665 - +0.5 1,933,671 - -0.0 1,933,738 - -0.4 1,933,779 + -1.8 1,933,801 - hemG Synpcc7942_1863 0.11 -0.0 1,933,824 + hemG Synpcc7942_1863 0.14 -1.0 1,933,837 - hemG Synpcc7942_1863 0.15 +1.0 1,933,842 - hemG Synpcc7942_1863 0.16 +1.0 1,933,852 + hemG Synpcc7942_1863 0.17 -0.0 1,933,857 + hemG Synpcc7942_1863 0.18 -1.7 1,933,857 + hemG Synpcc7942_1863 0.18 +0.6 1,933,862 + hemG Synpcc7942_1863 0.18 +1.0 1,933,870 - hemG Synpcc7942_1863 0.19 -1.8 1,933,928 + hemG Synpcc7942_1863 0.26 -0.6 1,933,928 + hemG Synpcc7942_1863 0.26 -0.6 1,933,928 + hemG Synpcc7942_1863 0.26 -0.0 1,933,936 - hemG Synpcc7942_1863 0.27 -1.4 1,933,936 - hemG Synpcc7942_1863 0.27 -2.0 1,933,936 - hemG Synpcc7942_1863 0.27 -1.3 1,933,936 - hemG Synpcc7942_1863 0.27 +1.0 1,933,947 - hemG Synpcc7942_1863 0.28 -0.8 1,933,991 - hemG Synpcc7942_1863 0.33 -0.4 1,934,039 - hemG Synpcc7942_1863 0.38 -0.0 1,934,044 - hemG Synpcc7942_1863 0.39 -0.0 1,934,076 + hemG Synpcc7942_1863 0.43 -2.4 1,934,076 + hemG Synpcc7942_1863 0.43 +1.4 1,934,076 + hemG Synpcc7942_1863 0.43 -0.4 1,934,084 - hemG Synpcc7942_1863 0.43 +1.0 1,934,084 - hemG Synpcc7942_1863 0.43 +1.0 1,934,146 + hemG Synpcc7942_1863 0.50 +1.0 1,934,178 + hemG Synpcc7942_1863 0.54 +1.0 1,934,183 + hemG Synpcc7942_1863 0.55 -1.0 1,934,191 - hemG Synpcc7942_1863 0.56 +0.6 1,934,225 + hemG Synpcc7942_1863 0.59 +1.0 1,934,226 + hemG Synpcc7942_1863 0.60 -0.0 1,934,229 - hemG Synpcc7942_1863 0.60 +2.6 1,934,234 + hemG Synpcc7942_1863 0.60 +1.0 1,934,240 + hemG Synpcc7942_1863 0.61 +2.0 1,934,260 + hemG Synpcc7942_1863 0.63 +1.0 1,934,330 - hemG Synpcc7942_1863 0.71 -0.0 1,934,344 + hemG Synpcc7942_1863 0.73 -0.6 1,934,344 + hemG Synpcc7942_1863 0.73 -0.0 1,934,344 + hemG Synpcc7942_1863 0.73 -1.6 1,934,352 - hemG Synpcc7942_1863 0.74 +0.2 1,934,352 - hemG Synpcc7942_1863 0.74 -1.6 1,934,362 - hemG Synpcc7942_1863 0.75 +1.0 1,934,374 + hemG Synpcc7942_1863 0.76 -1.0 1,934,391 + hemG Synpcc7942_1863 0.78 -0.9 1,934,440 + hemG Synpcc7942_1863 0.84 -1.4 1,934,440 + hemG Synpcc7942_1863 0.84 -0.6 1,934,440 + hemG Synpcc7942_1863 0.84 -0.6 1,934,465 - hemG Synpcc7942_1863 0.87 +1.0 1,934,520 - +0.2 1,934,529 - -1.0 1,934,567 + -0.8 1,934,577 + -0.4 1,934,577 + -1.1
Or see this region's nucleotide sequence