Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recN and mutL are separated by 73 nucleotides mutL and Synpcc7942_1781 are separated by 82 nucleotides Synpcc7942_1781 and thrC are separated by 85 nucleotides
Synpcc7942_1779: recN - DNA repair protein RecN, at 1,843,941 to 1,845,686
recN
Synpcc7942_1780: mutL - DNA mismatch repair protein, at 1,845,760 to 1,847,385
mutL
Synpcc7942_1781: Synpcc7942_1781 - hypothetical protein, at 1,847,468 to 1,847,743
_1781
Synpcc7942_1782: thrC - threonine synthase, at 1,847,829 to 1,849,121
thrC
Position (kb)
1845
1846
1847
1848 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1844.807 kb on + strand, within recN at 1844.826 kb on + strand, within recN at 1844.826 kb on + strand, within recN at 1844.826 kb on + strand, within recN at 1844.826 kb on + strand, within recN at 1844.828 kb on + strand, within recN at 1844.834 kb on + strand at 1844.834 kb on - strand at 1844.834 kb on - strand, within recN at 1844.834 kb on - strand, within recN at 1844.834 kb on - strand, within recN at 1844.834 kb on - strand, within recN at 1844.834 kb on - strand, within recN at 1844.910 kb on + strand, within recN at 1844.913 kb on - strand, within recN at 1844.918 kb on - strand, within recN at 1844.922 kb on + strand, within recN at 1844.929 kb on + strand, within recN at 1844.941 kb on + strand, within recN at 1844.943 kb on + strand, within recN at 1844.989 kb on + strand, within recN at 1844.989 kb on + strand, within recN at 1844.990 kb on + strand, within recN at 1845.027 kb on - strand, within recN at 1845.054 kb on + strand, within recN at 1845.079 kb on + strand, within recN at 1845.087 kb on - strand, within recN at 1845.144 kb on + strand, within recN at 1845.170 kb on - strand, within recN at 1845.178 kb on + strand, within recN at 1845.254 kb on - strand, within recN at 1845.280 kb on + strand, within recN at 1845.318 kb on + strand, within recN at 1845.318 kb on + strand, within recN at 1845.326 kb on - strand, within recN at 1845.343 kb on - strand, within recN at 1845.350 kb on + strand, within recN at 1845.353 kb on - strand, within recN at 1845.403 kb on + strand, within recN at 1845.434 kb on - strand, within recN at 1845.458 kb on + strand, within recN at 1845.479 kb on + strand, within recN at 1845.479 kb on + strand, within recN at 1845.614 kb on + strand at 1845.661 kb on + strand at 1845.675 kb on + strand at 1845.678 kb on - strand at 1845.683 kb on - strand at 1845.683 kb on - strand at 1845.690 kb on - strand at 1845.693 kb on - strand at 1845.717 kb on + strand at 1845.747 kb on - strand at 1845.795 kb on + strand at 1845.813 kb on - strand at 1845.815 kb on - strand at 1845.848 kb on - strand at 1845.859 kb on - strand at 1845.902 kb on - strand at 1845.914 kb on - strand at 1845.914 kb on - strand at 1845.924 kb on - strand, within mutL at 1845.954 kb on - strand, within mutL at 1845.982 kb on + strand, within mutL at 1845.982 kb on + strand, within mutL at 1845.988 kb on + strand, within mutL at 1845.995 kb on - strand, within mutL at 1845.996 kb on - strand, within mutL at 1846.044 kb on - strand, within mutL at 1846.057 kb on + strand, within mutL at 1846.062 kb on - strand, within mutL at 1846.064 kb on + strand, within mutL at 1846.064 kb on + strand, within mutL at 1846.064 kb on + strand, within mutL at 1846.064 kb on + strand, within mutL at 1846.064 kb on + strand, within mutL at 1846.065 kb on - strand, within mutL at 1846.072 kb on - strand, within mutL at 1846.072 kb on - strand, within mutL at 1846.072 kb on - strand, within mutL at 1846.072 kb on - strand, within mutL at 1846.152 kb on + strand, within mutL at 1846.160 kb on - strand, within mutL at 1846.185 kb on + strand, within mutL at 1846.203 kb on - strand, within mutL at 1846.209 kb on - strand, within mutL at 1846.223 kb on + strand at 1846.223 kb on + strand, within mutL at 1846.229 kb on + strand, within mutL at 1846.229 kb on + strand, within mutL at 1846.231 kb on - strand, within mutL at 1846.231 kb on - strand, within mutL at 1846.263 kb on + strand, within mutL at 1846.329 kb on - strand, within mutL at 1846.344 kb on + strand, within mutL at 1846.344 kb on + strand, within mutL at 1846.344 kb on + strand, within mutL at 1846.363 kb on - strand, within mutL at 1846.381 kb on - strand, within mutL at 1846.383 kb on - strand, within mutL at 1846.401 kb on + strand, within mutL at 1846.401 kb on + strand, within mutL at 1846.401 kb on + strand, within mutL at 1846.401 kb on + strand, within mutL at 1846.406 kb on + strand, within mutL at 1846.409 kb on - strand, within mutL at 1846.409 kb on - strand, within mutL at 1846.414 kb on - strand, within mutL at 1846.415 kb on - strand, within mutL at 1846.436 kb on + strand, within mutL at 1846.436 kb on + strand, within mutL at 1846.464 kb on - strand, within mutL at 1846.464 kb on - strand, within mutL at 1846.523 kb on - strand, within mutL at 1846.523 kb on - strand, within mutL at 1846.523 kb on - strand, within mutL at 1846.528 kb on + strand, within mutL at 1846.528 kb on + strand, within mutL at 1846.535 kb on + strand, within mutL at 1846.563 kb on - strand, within mutL at 1846.563 kb on - strand, within mutL at 1846.585 kb on + strand, within mutL at 1846.620 kb on - strand, within mutL at 1846.647 kb on - strand, within mutL at 1846.671 kb on + strand, within mutL at 1846.671 kb on + strand, within mutL at 1846.679 kb on - strand, within mutL at 1846.681 kb on + strand, within mutL at 1846.685 kb on - strand, within mutL at 1846.690 kb on + strand, within mutL at 1846.701 kb on + strand, within mutL at 1846.784 kb on + strand, within mutL at 1846.789 kb on + strand, within mutL at 1846.811 kb on + strand, within mutL at 1846.831 kb on - strand, within mutL at 1846.941 kb on + strand, within mutL at 1846.948 kb on - strand, within mutL at 1846.950 kb on + strand, within mutL at 1846.985 kb on + strand, within mutL at 1846.986 kb on + strand, within mutL at 1847.022 kb on + strand, within mutL at 1847.022 kb on + strand, within mutL at 1847.030 kb on - strand, within mutL at 1847.098 kb on + strand, within mutL at 1847.107 kb on - strand, within mutL at 1847.109 kb on + strand, within mutL at 1847.133 kb on + strand, within mutL at 1847.133 kb on + strand, within mutL at 1847.162 kb on + strand, within mutL at 1847.219 kb on + strand, within mutL at 1847.219 kb on + strand, within mutL at 1847.219 kb on + strand, within mutL at 1847.222 kb on - strand, within mutL at 1847.222 kb on - strand, within mutL at 1847.222 kb on - strand, within mutL at 1847.222 kb on - strand, within mutL at 1847.227 kb on - strand at 1847.227 kb on - strand at 1847.227 kb on - strand at 1847.227 kb on - strand at 1847.256 kb on + strand at 1847.269 kb on + strand at 1847.274 kb on + strand at 1847.277 kb on - strand at 1847.277 kb on - strand at 1847.310 kb on + strand at 1847.311 kb on + strand at 1847.319 kb on - strand at 1847.380 kb on + strand at 1847.410 kb on + strand at 1847.866 kb on + strand at 1847.866 kb on + strand at 1847.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 1,844,807 + recN Synpcc7942_1779 0.50 -0.8 1,844,826 + recN Synpcc7942_1779 0.51 +0.4 1,844,826 + recN Synpcc7942_1779 0.51 -0.7 1,844,826 + recN Synpcc7942_1779 0.51 -1.1 1,844,826 + recN Synpcc7942_1779 0.51 +1.0 1,844,828 + recN Synpcc7942_1779 0.51 +1.0 1,844,834 + -0.0 1,844,834 - +1.0 1,844,834 - recN Synpcc7942_1779 0.51 -0.8 1,844,834 - recN Synpcc7942_1779 0.51 -0.9 1,844,834 - recN Synpcc7942_1779 0.51 -0.5 1,844,834 - recN Synpcc7942_1779 0.51 +0.6 1,844,834 - recN Synpcc7942_1779 0.51 +1.4 1,844,910 + recN Synpcc7942_1779 0.55 +1.0 1,844,913 - recN Synpcc7942_1779 0.56 -0.7 1,844,918 - recN Synpcc7942_1779 0.56 -1.5 1,844,922 + recN Synpcc7942_1779 0.56 +0.3 1,844,929 + recN Synpcc7942_1779 0.57 -2.5 1,844,941 + recN Synpcc7942_1779 0.57 -0.3 1,844,943 + recN Synpcc7942_1779 0.57 +0.4 1,844,989 + recN Synpcc7942_1779 0.60 -2.0 1,844,989 + recN Synpcc7942_1779 0.60 -0.0 1,844,990 + recN Synpcc7942_1779 0.60 -1.6 1,845,027 - recN Synpcc7942_1779 0.62 -0.4 1,845,054 + recN Synpcc7942_1779 0.64 +0.6 1,845,079 + recN Synpcc7942_1779 0.65 -0.2 1,845,087 - recN Synpcc7942_1779 0.66 -0.3 1,845,144 + recN Synpcc7942_1779 0.69 +1.0 1,845,170 - recN Synpcc7942_1779 0.70 -4.4 1,845,178 + recN Synpcc7942_1779 0.71 +2.2 1,845,254 - recN Synpcc7942_1779 0.75 -0.6 1,845,280 + recN Synpcc7942_1779 0.77 +0.4 1,845,318 + recN Synpcc7942_1779 0.79 +0.2 1,845,318 + recN Synpcc7942_1779 0.79 -0.6 1,845,326 - recN Synpcc7942_1779 0.79 +1.0 1,845,343 - recN Synpcc7942_1779 0.80 +1.0 1,845,350 + recN Synpcc7942_1779 0.81 +2.6 1,845,353 - recN Synpcc7942_1779 0.81 +1.0 1,845,403 + recN Synpcc7942_1779 0.84 -1.2 1,845,434 - recN Synpcc7942_1779 0.86 +1.6 1,845,458 + recN Synpcc7942_1779 0.87 -0.3 1,845,479 + recN Synpcc7942_1779 0.88 -0.3 1,845,479 + recN Synpcc7942_1779 0.88 -1.2 1,845,614 + -0.8 1,845,661 + -2.5 1,845,675 + -2.7 1,845,678 - -1.3 1,845,683 - -1.2 1,845,683 - -0.8 1,845,690 - -0.4 1,845,693 - -1.1 1,845,717 + -0.9 1,845,747 - +0.2 1,845,795 + +1.0 1,845,813 - -1.8 1,845,815 - -0.9 1,845,848 - +1.7 1,845,859 - -1.2 1,845,902 - -0.8 1,845,914 - +1.6 1,845,914 - -1.2 1,845,924 - mutL Synpcc7942_1780 0.10 +0.1 1,845,954 - mutL Synpcc7942_1780 0.12 -0.5 1,845,982 + mutL Synpcc7942_1780 0.14 -0.6 1,845,982 + mutL Synpcc7942_1780 0.14 -2.7 1,845,988 + mutL Synpcc7942_1780 0.14 +1.1 1,845,995 - mutL Synpcc7942_1780 0.14 -0.8 1,845,996 - mutL Synpcc7942_1780 0.15 -2.6 1,846,044 - mutL Synpcc7942_1780 0.17 -0.9 1,846,057 + mutL Synpcc7942_1780 0.18 +2.0 1,846,062 - mutL Synpcc7942_1780 0.19 +1.0 1,846,064 + mutL Synpcc7942_1780 0.19 +1.0 1,846,064 + mutL Synpcc7942_1780 0.19 -1.8 1,846,064 + mutL Synpcc7942_1780 0.19 -0.3 1,846,064 + mutL Synpcc7942_1780 0.19 -0.0 1,846,064 + mutL Synpcc7942_1780 0.19 +0.5 1,846,065 - mutL Synpcc7942_1780 0.19 -1.0 1,846,072 - mutL Synpcc7942_1780 0.19 -0.5 1,846,072 - mutL Synpcc7942_1780 0.19 -1.4 1,846,072 - mutL Synpcc7942_1780 0.19 -1.4 1,846,072 - mutL Synpcc7942_1780 0.19 -0.4 1,846,152 + mutL Synpcc7942_1780 0.24 -1.8 1,846,160 - mutL Synpcc7942_1780 0.25 -0.9 1,846,185 + mutL Synpcc7942_1780 0.26 +1.0 1,846,203 - mutL Synpcc7942_1780 0.27 +1.1 1,846,209 - mutL Synpcc7942_1780 0.28 +1.6 1,846,223 + -0.6 1,846,223 + mutL Synpcc7942_1780 0.28 -0.6 1,846,229 + mutL Synpcc7942_1780 0.29 +0.1 1,846,229 + mutL Synpcc7942_1780 0.29 +1.2 1,846,231 - mutL Synpcc7942_1780 0.29 +1.0 1,846,231 - mutL Synpcc7942_1780 0.29 -0.2 1,846,263 + mutL Synpcc7942_1780 0.31 +0.2 1,846,329 - mutL Synpcc7942_1780 0.35 -0.0 1,846,344 + mutL Synpcc7942_1780 0.36 +1.3 1,846,344 + mutL Synpcc7942_1780 0.36 +1.0 1,846,344 + mutL Synpcc7942_1780 0.36 -2.2 1,846,363 - mutL Synpcc7942_1780 0.37 -0.1 1,846,381 - mutL Synpcc7942_1780 0.38 -0.0 1,846,383 - mutL Synpcc7942_1780 0.38 -1.9 1,846,401 + mutL Synpcc7942_1780 0.39 -0.8 1,846,401 + mutL Synpcc7942_1780 0.39 -0.0 1,846,401 + mutL Synpcc7942_1780 0.39 +1.3 1,846,401 + mutL Synpcc7942_1780 0.39 -0.7 1,846,406 + mutL Synpcc7942_1780 0.40 -1.8 1,846,409 - mutL Synpcc7942_1780 0.40 +2.6 1,846,409 - mutL Synpcc7942_1780 0.40 -2.5 1,846,414 - mutL Synpcc7942_1780 0.40 +1.4 1,846,415 - mutL Synpcc7942_1780 0.40 +1.0 1,846,436 + mutL Synpcc7942_1780 0.42 -2.1 1,846,436 + mutL Synpcc7942_1780 0.42 -1.2 1,846,464 - mutL Synpcc7942_1780 0.43 +1.0 1,846,464 - mutL Synpcc7942_1780 0.43 -0.6 1,846,523 - mutL Synpcc7942_1780 0.47 -0.0 1,846,523 - mutL Synpcc7942_1780 0.47 -0.3 1,846,523 - mutL Synpcc7942_1780 0.47 -1.5 1,846,528 + mutL Synpcc7942_1780 0.47 -0.3 1,846,528 + mutL Synpcc7942_1780 0.47 +1.9 1,846,535 + mutL Synpcc7942_1780 0.48 -0.3 1,846,563 - mutL Synpcc7942_1780 0.49 -1.2 1,846,563 - mutL Synpcc7942_1780 0.49 +0.5 1,846,585 + mutL Synpcc7942_1780 0.51 +0.5 1,846,620 - mutL Synpcc7942_1780 0.53 -1.3 1,846,647 - mutL Synpcc7942_1780 0.55 -1.7 1,846,671 + mutL Synpcc7942_1780 0.56 +1.6 1,846,671 + mutL Synpcc7942_1780 0.56 -0.7 1,846,679 - mutL Synpcc7942_1780 0.57 -0.3 1,846,681 + mutL Synpcc7942_1780 0.57 +1.0 1,846,685 - mutL Synpcc7942_1780 0.57 -0.6 1,846,690 + mutL Synpcc7942_1780 0.57 +1.2 1,846,701 + mutL Synpcc7942_1780 0.58 -0.8 1,846,784 + mutL Synpcc7942_1780 0.63 +0.4 1,846,789 + mutL Synpcc7942_1780 0.63 +1.2 1,846,811 + mutL Synpcc7942_1780 0.65 -1.1 1,846,831 - mutL Synpcc7942_1780 0.66 -1.2 1,846,941 + mutL Synpcc7942_1780 0.73 -0.0 1,846,948 - mutL Synpcc7942_1780 0.73 -0.2 1,846,950 + mutL Synpcc7942_1780 0.73 -2.1 1,846,985 + mutL Synpcc7942_1780 0.75 +1.0 1,846,986 + mutL Synpcc7942_1780 0.75 -1.4 1,847,022 + mutL Synpcc7942_1780 0.78 -0.6 1,847,022 + mutL Synpcc7942_1780 0.78 +1.4 1,847,030 - mutL Synpcc7942_1780 0.78 -0.6 1,847,098 + mutL Synpcc7942_1780 0.82 +4.1 1,847,107 - mutL Synpcc7942_1780 0.83 -2.7 1,847,109 + mutL Synpcc7942_1780 0.83 +0.1 1,847,133 + mutL Synpcc7942_1780 0.84 +0.3 1,847,133 + mutL Synpcc7942_1780 0.84 +1.3 1,847,162 + mutL Synpcc7942_1780 0.86 +1.0 1,847,219 + mutL Synpcc7942_1780 0.90 -1.2 1,847,219 + mutL Synpcc7942_1780 0.90 -0.6 1,847,219 + mutL Synpcc7942_1780 0.90 -2.4 1,847,222 - mutL Synpcc7942_1780 0.90 +1.0 1,847,222 - mutL Synpcc7942_1780 0.90 -0.6 1,847,222 - mutL Synpcc7942_1780 0.90 +2.0 1,847,222 - mutL Synpcc7942_1780 0.90 +2.6 1,847,227 - -1.5 1,847,227 - +1.1 1,847,227 - -1.9 1,847,227 - +0.1 1,847,256 + -1.3 1,847,269 + -0.5 1,847,274 + +0.6 1,847,277 - -0.6 1,847,277 - -1.2 1,847,310 + -2.1 1,847,311 + +3.0 1,847,319 - +1.4 1,847,380 + -1.0 1,847,410 + -0.7 1,847,866 + -1.1 1,847,866 + +0.6 1,847,868 + -0.8
Or see this region's nucleotide sequence