Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt katG and Synpcc7942_1657 are separated by 371 nucleotides Synpcc7942_1657 and Synpcc7942_1658 are separated by 113 nucleotides Synpcc7942_1658 and Synpcc7942_1659 are separated by 137 nucleotides
Synpcc7942_1656: katG - catalase/peroxidase HPI, at 1,725,919 to 1,728,081
katG
Synpcc7942_1657: Synpcc7942_1657 - hypothetical protein, at 1,728,453 to 1,728,674
_1657
Synpcc7942_1658: Synpcc7942_1658 - hypothetical protein, at 1,728,788 to 1,729,246
_1658
Synpcc7942_1659: Synpcc7942_1659 - nitroreductase, at 1,729,384 to 1,730,031
_1659
Position (kb)
1728
1729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1727.468 kb on + strand, within katG at 1727.476 kb on - strand, within katG at 1727.476 kb on - strand, within katG at 1727.476 kb on - strand, within katG at 1727.482 kb on + strand, within katG at 1727.507 kb on - strand, within katG at 1727.625 kb on - strand, within katG at 1727.695 kb on + strand at 1727.735 kb on + strand, within katG at 1727.767 kb on + strand, within katG at 1727.823 kb on - strand, within katG at 1727.895 kb on + strand at 1728.046 kb on + strand at 1728.206 kb on - strand at 1728.243 kb on - strand at 1728.245 kb on + strand at 1728.250 kb on - strand at 1728.281 kb on - strand at 1728.378 kb on + strand at 1728.401 kb on - strand at 1728.405 kb on + strand at 1728.408 kb on - strand at 1728.410 kb on + strand at 1728.421 kb on + strand at 1728.422 kb on + strand at 1728.422 kb on + strand at 1728.422 kb on + strand at 1728.512 kb on + strand, within Synpcc7942_1657 at 1728.515 kb on + strand, within Synpcc7942_1657 at 1728.535 kb on - strand, within Synpcc7942_1657 at 1728.548 kb on + strand, within Synpcc7942_1657 at 1728.569 kb on - strand, within Synpcc7942_1657 at 1728.569 kb on - strand, within Synpcc7942_1657 at 1728.643 kb on + strand, within Synpcc7942_1657 at 1728.643 kb on + strand, within Synpcc7942_1657 at 1728.648 kb on + strand, within Synpcc7942_1657 at 1728.661 kb on - strand at 1728.715 kb on + strand at 1728.733 kb on - strand at 1728.735 kb on + strand at 1728.735 kb on + strand at 1728.743 kb on - strand at 1728.743 kb on - strand at 1728.751 kb on - strand at 1728.785 kb on - strand at 1728.875 kb on + strand, within Synpcc7942_1658 at 1728.875 kb on + strand, within Synpcc7942_1658 at 1728.883 kb on - strand, within Synpcc7942_1658 at 1728.951 kb on - strand, within Synpcc7942_1658 at 1728.951 kb on - strand at 1728.955 kb on + strand, within Synpcc7942_1658 at 1728.965 kb on + strand, within Synpcc7942_1658 at 1728.982 kb on + strand, within Synpcc7942_1658 at 1728.999 kb on + strand, within Synpcc7942_1658 at 1729.002 kb on - strand, within Synpcc7942_1658 at 1729.007 kb on - strand, within Synpcc7942_1658 at 1729.018 kb on - strand, within Synpcc7942_1658 at 1729.030 kb on - strand, within Synpcc7942_1658 at 1729.034 kb on + strand, within Synpcc7942_1658 at 1729.034 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.100 kb on + strand, within Synpcc7942_1658 at 1729.108 kb on - strand, within Synpcc7942_1658 at 1729.163 kb on - strand, within Synpcc7942_1658 at 1729.186 kb on + strand, within Synpcc7942_1658 at 1729.188 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.217 kb on - strand at 1729.223 kb on + strand at 1729.223 kb on + strand at 1729.223 kb on + strand at 1729.231 kb on - strand at 1729.244 kb on - strand at 1729.255 kb on - strand at 1729.272 kb on - strand at 1729.293 kb on - strand at 1729.311 kb on + strand at 1729.311 kb on + strand at 1729.319 kb on - strand at 1729.340 kb on + strand at 1729.359 kb on - strand at 1729.359 kb on - strand at 1729.361 kb on + strand at 1729.374 kb on - strand at 1729.387 kb on - strand at 1729.415 kb on - strand at 1729.423 kb on - strand at 1729.483 kb on - strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.501 kb on + strand, within Synpcc7942_1659 at 1729.504 kb on - strand, within Synpcc7942_1659 at 1729.504 kb on - strand, within Synpcc7942_1659 at 1729.514 kb on + strand, within Synpcc7942_1659 at 1729.520 kb on - strand, within Synpcc7942_1659 at 1729.531 kb on - strand, within Synpcc7942_1659 at 1729.532 kb on - strand, within Synpcc7942_1659 at 1729.556 kb on + strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.599 kb on + strand, within Synpcc7942_1659 at 1729.606 kb on - strand, within Synpcc7942_1659 at 1729.618 kb on - strand, within Synpcc7942_1659 at 1729.651 kb on - strand, within Synpcc7942_1659 at 1729.659 kb on + strand, within Synpcc7942_1659 at 1729.663 kb on - strand, within Synpcc7942_1659
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 1,727,468 + katG Synpcc7942_1656 0.72 +1.3 1,727,476 - katG Synpcc7942_1656 0.72 -2.5 1,727,476 - katG Synpcc7942_1656 0.72 +0.1 1,727,476 - katG Synpcc7942_1656 0.72 +1.0 1,727,482 + katG Synpcc7942_1656 0.72 -0.8 1,727,507 - katG Synpcc7942_1656 0.73 -0.3 1,727,625 - katG Synpcc7942_1656 0.79 -1.6 1,727,695 + +1.0 1,727,735 + katG Synpcc7942_1656 0.84 +1.0 1,727,767 + katG Synpcc7942_1656 0.85 +0.0 1,727,823 - katG Synpcc7942_1656 0.88 -2.0 1,727,895 + -1.2 1,728,046 + +1.0 1,728,206 - -0.8 1,728,243 - -1.7 1,728,245 + -0.3 1,728,250 - -0.5 1,728,281 - -1.6 1,728,378 + -1.2 1,728,401 - -0.7 1,728,405 + +1.0 1,728,408 - -1.3 1,728,410 + -1.1 1,728,421 + -1.6 1,728,422 + -0.6 1,728,422 + -3.1 1,728,422 + +0.6 1,728,512 + Synpcc7942_1657 0.27 +3.0 1,728,515 + Synpcc7942_1657 0.28 -0.7 1,728,535 - Synpcc7942_1657 0.37 -0.6 1,728,548 + Synpcc7942_1657 0.43 -3.2 1,728,569 - Synpcc7942_1657 0.52 -0.6 1,728,569 - Synpcc7942_1657 0.52 -0.6 1,728,643 + Synpcc7942_1657 0.86 -0.6 1,728,643 + Synpcc7942_1657 0.86 -0.6 1,728,648 + Synpcc7942_1657 0.88 -0.9 1,728,661 - -1.0 1,728,715 + -1.3 1,728,733 - +2.9 1,728,735 + +1.9 1,728,735 + -0.2 1,728,743 - -2.6 1,728,743 - -2.8 1,728,751 - +0.0 1,728,785 - +1.6 1,728,875 + Synpcc7942_1658 0.19 +0.0 1,728,875 + Synpcc7942_1658 0.19 -0.8 1,728,883 - Synpcc7942_1658 0.21 -0.6 1,728,951 - Synpcc7942_1658 0.36 +0.7 1,728,951 - -1.8 1,728,955 + Synpcc7942_1658 0.36 +1.0 1,728,965 + Synpcc7942_1658 0.39 +1.0 1,728,982 + Synpcc7942_1658 0.42 -0.6 1,728,999 + Synpcc7942_1658 0.46 -0.1 1,729,002 - Synpcc7942_1658 0.47 -0.6 1,729,007 - Synpcc7942_1658 0.48 -0.8 1,729,018 - Synpcc7942_1658 0.50 +0.0 1,729,030 - Synpcc7942_1658 0.53 -1.3 1,729,034 + Synpcc7942_1658 0.54 -2.5 1,729,034 + Synpcc7942_1658 0.54 -0.6 1,729,045 + Synpcc7942_1658 0.56 +0.0 1,729,045 + Synpcc7942_1658 0.56 +0.0 1,729,045 + Synpcc7942_1658 0.56 -1.6 1,729,045 + Synpcc7942_1658 0.56 +1.0 1,729,100 + Synpcc7942_1658 0.68 -0.6 1,729,108 - Synpcc7942_1658 0.70 -0.6 1,729,163 - Synpcc7942_1658 0.82 -1.6 1,729,186 + Synpcc7942_1658 0.87 +0.0 1,729,188 - Synpcc7942_1658 0.87 +0.6 1,729,194 - Synpcc7942_1658 0.88 +0.6 1,729,194 - Synpcc7942_1658 0.88 +1.0 1,729,194 - Synpcc7942_1658 0.88 +1.2 1,729,217 - +1.0 1,729,223 + +0.6 1,729,223 + +1.0 1,729,223 + -1.0 1,729,231 - -2.0 1,729,244 - +2.0 1,729,255 - -1.2 1,729,272 - +0.0 1,729,293 - -0.5 1,729,311 + -0.6 1,729,311 + -2.0 1,729,319 - +1.0 1,729,340 + +1.0 1,729,359 - -1.2 1,729,359 - +0.0 1,729,361 + +1.3 1,729,374 - +1.0 1,729,387 - -1.1 1,729,415 - -1.3 1,729,423 - +1.3 1,729,483 - Synpcc7942_1659 0.15 -0.2 1,729,496 + Synpcc7942_1659 0.17 -1.9 1,729,496 + Synpcc7942_1659 0.17 -0.2 1,729,496 + Synpcc7942_1659 0.17 +2.1 1,729,501 + Synpcc7942_1659 0.18 -0.8 1,729,504 - Synpcc7942_1659 0.19 -1.8 1,729,504 - Synpcc7942_1659 0.19 +1.6 1,729,514 + Synpcc7942_1659 0.20 -0.3 1,729,520 - Synpcc7942_1659 0.21 -2.2 1,729,531 - Synpcc7942_1659 0.23 +1.0 1,729,532 - Synpcc7942_1659 0.23 -0.6 1,729,556 + Synpcc7942_1659 0.27 -3.0 1,729,564 - Synpcc7942_1659 0.28 +0.0 1,729,564 - Synpcc7942_1659 0.28 -2.2 1,729,564 - Synpcc7942_1659 0.28 +1.0 1,729,564 - Synpcc7942_1659 0.28 +0.2 1,729,589 + Synpcc7942_1659 0.32 -1.3 1,729,589 + Synpcc7942_1659 0.32 -0.2 1,729,589 + Synpcc7942_1659 0.32 +1.0 1,729,589 + Synpcc7942_1659 0.32 -0.9 1,729,597 - Synpcc7942_1659 0.33 +1.2 1,729,597 - Synpcc7942_1659 0.33 +0.0 1,729,597 - Synpcc7942_1659 0.33 -0.2 1,729,597 - Synpcc7942_1659 0.33 -2.3 1,729,599 + Synpcc7942_1659 0.33 +1.6 1,729,606 - Synpcc7942_1659 0.34 +1.0 1,729,618 - Synpcc7942_1659 0.36 -1.7 1,729,651 - Synpcc7942_1659 0.41 +0.4 1,729,659 + Synpcc7942_1659 0.42 -1.3 1,729,663 - Synpcc7942_1659 0.43 -2.0
Or see this region's nucleotide sequence