Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxD and pstB are separated by 64 nucleotides pstB and Synpcc7942_1451 are separated by 42 nucleotides Synpcc7942_1451 and radA overlap by 4 nucleotides
Synpcc7942_1449: lpxD - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, at 1,502,325 to 1,503,392
lpxD
Synpcc7942_1450: pstB - ATPase, at 1,503,457 to 1,504,257
pstB
Synpcc7942_1451: Synpcc7942_1451 - hypothetical protein, at 1,504,300 to 1,504,761
_1451
Synpcc7942_1452: radA - DNA repair protein RadA, at 1,504,758 to 1,506,194
radA
Position (kb) 1503
1504
1505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1503.394 kb on + strand at 1503.394 kb on + strand at 1503.435 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.440 kb on + strand at 1503.442 kb on - strand at 1503.443 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.448 kb on - strand at 1503.460 kb on - strand at 1503.462 kb on + strand at 1503.480 kb on + strand at 1503.518 kb on + strand at 1503.520 kb on + strand at 1503.529 kb on + strand at 1503.537 kb on - strand, within pstB at 1503.606 kb on - strand, within pstB at 1503.609 kb on + strand, within pstB at 1503.611 kb on + strand, within pstB at 1503.611 kb on + strand, within pstB at 1503.622 kb on + strand, within pstB at 1503.632 kb on + strand, within pstB at 1503.640 kb on - strand, within pstB at 1503.640 kb on - strand, within pstB at 1503.640 kb on - strand, within pstB at 1503.640 kb on - strand, within pstB at 1503.656 kb on + strand, within pstB at 1503.661 kb on + strand, within pstB at 1503.695 kb on + strand, within pstB at 1503.740 kb on + strand, within pstB at 1503.748 kb on + strand, within pstB at 1503.756 kb on - strand, within pstB at 1503.756 kb on - strand, within pstB at 1503.756 kb on - strand, within pstB at 1503.840 kb on + strand, within pstB at 1503.859 kb on + strand, within pstB at 1503.869 kb on + strand, within pstB at 1503.879 kb on - strand, within pstB at 1503.917 kb on + strand, within pstB at 1503.917 kb on + strand, within pstB at 1503.917 kb on + strand, within pstB at 1503.936 kb on - strand, within pstB at 1503.943 kb on + strand, within pstB at 1503.943 kb on + strand at 1503.984 kb on - strand, within pstB at 1503.986 kb on + strand, within pstB at 1504.008 kb on + strand, within pstB at 1504.021 kb on + strand, within pstB at 1504.029 kb on - strand at 1504.029 kb on - strand, within pstB at 1504.029 kb on - strand, within pstB at 1504.029 kb on - strand, within pstB at 1504.058 kb on - strand, within pstB at 1504.072 kb on - strand, within pstB at 1504.072 kb on - strand, within pstB at 1504.075 kb on - strand, within pstB at 1504.118 kb on + strand, within pstB at 1504.121 kb on - strand, within pstB at 1504.123 kb on + strand, within pstB at 1504.123 kb on + strand, within pstB at 1504.128 kb on + strand, within pstB at 1504.128 kb on + strand, within pstB at 1504.131 kb on - strand, within pstB at 1504.131 kb on - strand, within pstB at 1504.131 kb on - strand, within pstB at 1504.133 kb on + strand, within pstB at 1504.136 kb on - strand, within pstB at 1504.141 kb on - strand, within pstB at 1504.169 kb on - strand, within pstB at 1504.180 kb on - strand at 1504.210 kb on - strand at 1504.259 kb on - strand at 1504.317 kb on + strand at 1504.363 kb on + strand, within Synpcc7942_1451 at 1504.371 kb on - strand, within Synpcc7942_1451 at 1504.371 kb on - strand, within Synpcc7942_1451 at 1504.374 kb on + strand, within Synpcc7942_1451 at 1504.381 kb on + strand, within Synpcc7942_1451 at 1504.386 kb on + strand, within Synpcc7942_1451 at 1504.386 kb on + strand, within Synpcc7942_1451 at 1504.386 kb on + strand, within Synpcc7942_1451 at 1504.393 kb on - strand, within Synpcc7942_1451 at 1504.394 kb on - strand, within Synpcc7942_1451 at 1504.394 kb on - strand, within Synpcc7942_1451 at 1504.406 kb on + strand, within Synpcc7942_1451 at 1504.408 kb on + strand, within Synpcc7942_1451 at 1504.416 kb on + strand, within Synpcc7942_1451 at 1504.421 kb on + strand, within Synpcc7942_1451 at 1504.421 kb on - strand, within Synpcc7942_1451 at 1504.431 kb on - strand, within Synpcc7942_1451 at 1504.439 kb on - strand, within Synpcc7942_1451 at 1504.441 kb on - strand, within Synpcc7942_1451 at 1504.449 kb on - strand, within Synpcc7942_1451 at 1504.454 kb on - strand, within Synpcc7942_1451 at 1504.473 kb on + strand, within Synpcc7942_1451 at 1504.489 kb on - strand, within Synpcc7942_1451 at 1504.498 kb on + strand, within Synpcc7942_1451 at 1504.529 kb on - strand, within Synpcc7942_1451 at 1504.548 kb on + strand, within Synpcc7942_1451 at 1504.548 kb on + strand, within Synpcc7942_1451 at 1504.548 kb on + strand, within Synpcc7942_1451 at 1504.556 kb on - strand, within Synpcc7942_1451 at 1504.556 kb on - strand, within Synpcc7942_1451 at 1504.599 kb on - strand, within Synpcc7942_1451 at 1504.602 kb on + strand, within Synpcc7942_1451 at 1504.602 kb on + strand, within Synpcc7942_1451 at 1504.610 kb on - strand, within Synpcc7942_1451 at 1504.679 kb on + strand, within Synpcc7942_1451 at 1504.689 kb on + strand, within Synpcc7942_1451 at 1504.697 kb on - strand, within Synpcc7942_1451 at 1504.697 kb on - strand, within Synpcc7942_1451 at 1504.712 kb on + strand, within Synpcc7942_1451 at 1504.793 kb on + strand at 1504.793 kb on + strand at 1504.793 kb on + strand at 1504.793 kb on + strand at 1504.793 kb on + strand at 1504.801 kb on - strand at 1504.857 kb on + strand at 1504.857 kb on + strand at 1504.869 kb on + strand at 1504.869 kb on + strand at 1504.869 kb on + strand at 1504.877 kb on - strand at 1504.907 kb on + strand, within radA at 1504.907 kb on + strand, within radA at 1504.907 kb on + strand, within radA at 1504.930 kb on - strand at 1504.936 kb on - strand, within radA at 1504.941 kb on + strand, within radA at 1504.949 kb on - strand, within radA at 1504.981 kb on - strand, within radA at 1505.010 kb on + strand, within radA at 1505.031 kb on + strand, within radA at 1505.061 kb on + strand, within radA at 1505.100 kb on - strand, within radA at 1505.103 kb on + strand, within radA at 1505.111 kb on - strand, within radA at 1505.111 kb on - strand, within radA at 1505.151 kb on + strand, within radA at 1505.157 kb on - strand, within radA at 1505.168 kb on + strand, within radA at 1505.187 kb on + strand, within radA at 1505.199 kb on - strand, within radA at 1505.227 kb on - strand, within radA at 1505.229 kb on - strand, within radA at 1505.234 kb on + strand, within radA at 1505.238 kb on + strand, within radA at 1505.247 kb on - strand, within radA
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 1,503,394 + -0.7 1,503,394 + +1.0 1,503,435 + -0.9 1,503,440 + +0.2 1,503,440 + +0.0 1,503,440 + -2.1 1,503,440 + -0.5 1,503,440 + -1.4 1,503,440 + -0.6 1,503,440 + +0.3 1,503,440 + +0.0 1,503,440 + -1.0 1,503,440 + -0.6 1,503,440 + +3.4 1,503,440 + +0.2 1,503,442 - +0.0 1,503,443 - -1.6 1,503,448 - -1.5 1,503,448 - +1.0 1,503,448 - -2.3 1,503,448 - +2.3 1,503,448 - +0.7 1,503,448 - -1.0 1,503,448 - -0.2 1,503,448 - +0.0 1,503,448 - +2.6 1,503,460 - -0.5 1,503,462 + +1.0 1,503,480 + -0.4 1,503,518 + -1.2 1,503,520 + +0.0 1,503,529 + -1.1 1,503,537 - pstB Synpcc7942_1450 0.10 -1.6 1,503,606 - pstB Synpcc7942_1450 0.19 -1.1 1,503,609 + pstB Synpcc7942_1450 0.19 -1.9 1,503,611 + pstB Synpcc7942_1450 0.19 +3.9 1,503,611 + pstB Synpcc7942_1450 0.19 -2.2 1,503,622 + pstB Synpcc7942_1450 0.21 -0.6 1,503,632 + pstB Synpcc7942_1450 0.22 -0.2 1,503,640 - pstB Synpcc7942_1450 0.23 -1.7 1,503,640 - pstB Synpcc7942_1450 0.23 -2.4 1,503,640 - pstB Synpcc7942_1450 0.23 -0.5 1,503,640 - pstB Synpcc7942_1450 0.23 -0.4 1,503,656 + pstB Synpcc7942_1450 0.25 -0.9 1,503,661 + pstB Synpcc7942_1450 0.25 -3.2 1,503,695 + pstB Synpcc7942_1450 0.30 -2.2 1,503,740 + pstB Synpcc7942_1450 0.35 -1.7 1,503,748 + pstB Synpcc7942_1450 0.36 -2.2 1,503,756 - pstB Synpcc7942_1450 0.37 -1.5 1,503,756 - pstB Synpcc7942_1450 0.37 +1.7 1,503,756 - pstB Synpcc7942_1450 0.37 -0.0 1,503,840 + pstB Synpcc7942_1450 0.48 +1.0 1,503,859 + pstB Synpcc7942_1450 0.50 -0.8 1,503,869 + pstB Synpcc7942_1450 0.51 +1.0 1,503,879 - pstB Synpcc7942_1450 0.53 -1.5 1,503,917 + pstB Synpcc7942_1450 0.57 -2.3 1,503,917 + pstB Synpcc7942_1450 0.57 -3.0 1,503,917 + pstB Synpcc7942_1450 0.57 -0.7 1,503,936 - pstB Synpcc7942_1450 0.60 -1.5 1,503,943 + pstB Synpcc7942_1450 0.61 -1.0 1,503,943 + -1.0 1,503,984 - pstB Synpcc7942_1450 0.66 -1.4 1,503,986 + pstB Synpcc7942_1450 0.66 +1.0 1,504,008 + pstB Synpcc7942_1450 0.69 -1.3 1,504,021 + pstB Synpcc7942_1450 0.70 -1.4 1,504,029 - -2.8 1,504,029 - pstB Synpcc7942_1450 0.71 -2.2 1,504,029 - pstB Synpcc7942_1450 0.71 -0.6 1,504,029 - pstB Synpcc7942_1450 0.71 -0.9 1,504,058 - pstB Synpcc7942_1450 0.75 +1.3 1,504,072 - pstB Synpcc7942_1450 0.77 -1.2 1,504,072 - pstB Synpcc7942_1450 0.77 -0.3 1,504,075 - pstB Synpcc7942_1450 0.77 -1.6 1,504,118 + pstB Synpcc7942_1450 0.83 -0.8 1,504,121 - pstB Synpcc7942_1450 0.83 -2.2 1,504,123 + pstB Synpcc7942_1450 0.83 -1.5 1,504,123 + pstB Synpcc7942_1450 0.83 -2.4 1,504,128 + pstB Synpcc7942_1450 0.84 -1.1 1,504,128 + pstB Synpcc7942_1450 0.84 -1.6 1,504,131 - pstB Synpcc7942_1450 0.84 -1.3 1,504,131 - pstB Synpcc7942_1450 0.84 -1.7 1,504,131 - pstB Synpcc7942_1450 0.84 -0.7 1,504,133 + pstB Synpcc7942_1450 0.84 -1.0 1,504,136 - pstB Synpcc7942_1450 0.85 -2.5 1,504,141 - pstB Synpcc7942_1450 0.85 +0.7 1,504,169 - pstB Synpcc7942_1450 0.89 -0.8 1,504,180 - +2.4 1,504,210 - -0.8 1,504,259 - -2.1 1,504,317 + +0.0 1,504,363 + Synpcc7942_1451 0.14 +0.7 1,504,371 - Synpcc7942_1451 0.15 -1.0 1,504,371 - Synpcc7942_1451 0.15 +0.6 1,504,374 + Synpcc7942_1451 0.16 -1.7 1,504,381 + Synpcc7942_1451 0.18 -0.5 1,504,386 + Synpcc7942_1451 0.19 -0.9 1,504,386 + Synpcc7942_1451 0.19 +0.7 1,504,386 + Synpcc7942_1451 0.19 -0.3 1,504,393 - Synpcc7942_1451 0.20 -1.0 1,504,394 - Synpcc7942_1451 0.20 +0.9 1,504,394 - Synpcc7942_1451 0.20 -1.9 1,504,406 + Synpcc7942_1451 0.23 +1.4 1,504,408 + Synpcc7942_1451 0.23 +0.4 1,504,416 + Synpcc7942_1451 0.25 -0.7 1,504,421 + Synpcc7942_1451 0.26 -0.8 1,504,421 - Synpcc7942_1451 0.26 -1.4 1,504,431 - Synpcc7942_1451 0.28 -0.6 1,504,439 - Synpcc7942_1451 0.30 -0.2 1,504,441 - Synpcc7942_1451 0.31 +1.0 1,504,449 - Synpcc7942_1451 0.32 +0.3 1,504,454 - Synpcc7942_1451 0.33 +0.0 1,504,473 + Synpcc7942_1451 0.37 -1.3 1,504,489 - Synpcc7942_1451 0.41 -1.2 1,504,498 + Synpcc7942_1451 0.43 +1.4 1,504,529 - Synpcc7942_1451 0.50 +1.0 1,504,548 + Synpcc7942_1451 0.54 -1.3 1,504,548 + Synpcc7942_1451 0.54 -1.4 1,504,548 + Synpcc7942_1451 0.54 +2.0 1,504,556 - Synpcc7942_1451 0.55 -2.6 1,504,556 - Synpcc7942_1451 0.55 -0.8 1,504,599 - Synpcc7942_1451 0.65 +2.0 1,504,602 + Synpcc7942_1451 0.65 -0.2 1,504,602 + Synpcc7942_1451 0.65 -0.2 1,504,610 - Synpcc7942_1451 0.67 -0.7 1,504,679 + Synpcc7942_1451 0.82 -0.6 1,504,689 + Synpcc7942_1451 0.84 +1.0 1,504,697 - Synpcc7942_1451 0.86 +0.9 1,504,697 - Synpcc7942_1451 0.86 +0.6 1,504,712 + Synpcc7942_1451 0.89 -0.6 1,504,793 + +0.2 1,504,793 + -2.3 1,504,793 + -0.6 1,504,793 + +1.0 1,504,793 + +1.0 1,504,801 - -1.4 1,504,857 + +1.3 1,504,857 + -0.2 1,504,869 + -0.1 1,504,869 + -0.6 1,504,869 + -0.8 1,504,877 - +1.0 1,504,907 + radA Synpcc7942_1452 0.10 +1.5 1,504,907 + radA Synpcc7942_1452 0.10 +2.0 1,504,907 + radA Synpcc7942_1452 0.10 -1.3 1,504,930 - -1.3 1,504,936 - radA Synpcc7942_1452 0.12 +0.4 1,504,941 + radA Synpcc7942_1452 0.13 -2.3 1,504,949 - radA Synpcc7942_1452 0.13 +0.0 1,504,981 - radA Synpcc7942_1452 0.16 -1.4 1,505,010 + radA Synpcc7942_1452 0.18 +1.4 1,505,031 + radA Synpcc7942_1452 0.19 -0.6 1,505,061 + radA Synpcc7942_1452 0.21 +1.9 1,505,100 - radA Synpcc7942_1452 0.24 -0.2 1,505,103 + radA Synpcc7942_1452 0.24 +1.0 1,505,111 - radA Synpcc7942_1452 0.25 +0.3 1,505,111 - radA Synpcc7942_1452 0.25 -0.6 1,505,151 + radA Synpcc7942_1452 0.27 +2.0 1,505,157 - radA Synpcc7942_1452 0.28 +1.0 1,505,168 + radA Synpcc7942_1452 0.29 -1.4 1,505,187 + radA Synpcc7942_1452 0.30 +1.6 1,505,199 - radA Synpcc7942_1452 0.31 -1.0 1,505,227 - radA Synpcc7942_1452 0.33 +3.0 1,505,229 - radA Synpcc7942_1452 0.33 +1.0 1,505,234 + radA Synpcc7942_1452 0.33 -1.9 1,505,238 + radA Synpcc7942_1452 0.33 +1.0 1,505,247 - radA Synpcc7942_1452 0.34 -1.4
Or see this region's nucleotide sequence