Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaG and pheT are separated by 142 nucleotides pheT and Synpcc7942_1294 are separated by 135 nucleotides
Synpcc7942_1292: dnaG - DNA primase, at 1,313,939 to 1,316,023
dnaG
Synpcc7942_1293: pheT - phenylalanyl-tRNA synthetase subunit beta, at 1,316,166 to 1,318,598
pheT
Synpcc7942_1294: Synpcc7942_1294 - serine/threonine protein kinase, at 1,318,734 to 1,320,302
_1294
Position (kb)
1316
1317
1318
1319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1316.029 kb on + strand at 1316.046 kb on - strand at 1316.069 kb on - strand at 1316.071 kb on + strand at 1316.079 kb on - strand at 1316.138 kb on + strand at 1316.139 kb on - strand at 1316.153 kb on + strand at 1316.161 kb on + strand at 1316.161 kb on - strand at 1316.161 kb on - strand at 1316.163 kb on + strand at 1316.163 kb on + strand at 1316.170 kb on - strand at 1316.170 kb on - strand at 1316.170 kb on - strand at 1316.170 kb on - strand at 1316.171 kb on - strand at 1318.651 kb on + strand at 1318.652 kb on + strand at 1318.662 kb on - strand at 1318.662 kb on - strand at 1318.689 kb on - strand at 1318.707 kb on - strand at 1318.718 kb on + strand at 1318.718 kb on + strand at 1318.721 kb on - strand at 1318.726 kb on - strand at 1318.738 kb on - strand at 1318.766 kb on + strand at 1318.782 kb on + strand at 1318.831 kb on - strand at 1318.874 kb on + strand at 1318.879 kb on + strand at 1318.887 kb on - strand at 1318.913 kb on - strand, within Synpcc7942_1294 at 1318.918 kb on + strand, within Synpcc7942_1294 at 1318.921 kb on - strand, within Synpcc7942_1294 at 1318.944 kb on + strand, within Synpcc7942_1294 at 1318.945 kb on - strand, within Synpcc7942_1294 at 1318.952 kb on - strand, within Synpcc7942_1294 at 1318.952 kb on - strand, within Synpcc7942_1294 at 1318.990 kb on - strand, within Synpcc7942_1294 at 1319.001 kb on + strand, within Synpcc7942_1294 at 1319.031 kb on + strand, within Synpcc7942_1294 at 1319.038 kb on + strand, within Synpcc7942_1294 at 1319.040 kb on - strand, within Synpcc7942_1294 at 1319.104 kb on + strand, within Synpcc7942_1294 at 1319.208 kb on - strand, within Synpcc7942_1294 at 1319.208 kb on - strand, within Synpcc7942_1294 at 1319.222 kb on + strand, within Synpcc7942_1294 at 1319.271 kb on - strand, within Synpcc7942_1294 at 1319.289 kb on + strand, within Synpcc7942_1294 at 1319.289 kb on + strand, within Synpcc7942_1294 at 1319.289 kb on + strand, within Synpcc7942_1294 at 1319.297 kb on - strand, within Synpcc7942_1294 at 1319.309 kb on + strand, within Synpcc7942_1294 at 1319.313 kb on - strand, within Synpcc7942_1294 at 1319.320 kb on + strand, within Synpcc7942_1294 at 1319.334 kb on + strand, within Synpcc7942_1294 at 1319.342 kb on - strand, within Synpcc7942_1294 at 1319.408 kb on - strand, within Synpcc7942_1294 at 1319.414 kb on + strand, within Synpcc7942_1294 at 1319.415 kb on + strand, within Synpcc7942_1294 at 1319.422 kb on + strand, within Synpcc7942_1294 at 1319.422 kb on - strand, within Synpcc7942_1294 at 1319.501 kb on + strand, within Synpcc7942_1294 at 1319.501 kb on + strand, within Synpcc7942_1294 at 1319.512 kb on + strand, within Synpcc7942_1294 at 1319.522 kb on + strand, within Synpcc7942_1294 at 1319.522 kb on + strand, within Synpcc7942_1294 at 1319.522 kb on + strand, within Synpcc7942_1294 at 1319.527 kb on + strand, within Synpcc7942_1294 at 1319.530 kb on - strand, within Synpcc7942_1294 at 1319.530 kb on - strand, within Synpcc7942_1294 at 1319.530 kb on - strand, within Synpcc7942_1294 at 1319.530 kb on - strand, within Synpcc7942_1294 at 1319.530 kb on - strand at 1319.530 kb on - strand, within Synpcc7942_1294
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 1,316,029 + +0.0 1,316,046 - +1.0 1,316,069 - +0.1 1,316,071 + -1.4 1,316,079 - +1.3 1,316,138 + +2.0 1,316,139 - +0.8 1,316,153 + -0.5 1,316,161 + +2.6 1,316,161 - +1.0 1,316,161 - -2.3 1,316,163 + -1.0 1,316,163 + +0.5 1,316,170 - -0.9 1,316,170 - -0.4 1,316,170 - -0.1 1,316,170 - +2.9 1,316,171 - +1.0 1,318,651 + -0.4 1,318,652 + -0.0 1,318,662 - -1.2 1,318,662 - +2.6 1,318,689 - +1.0 1,318,707 - +0.0 1,318,718 + +1.0 1,318,718 + -1.7 1,318,721 - -1.0 1,318,726 - -1.9 1,318,738 - -1.7 1,318,766 + +2.6 1,318,782 + -1.8 1,318,831 - +0.8 1,318,874 + +0.9 1,318,879 + -0.5 1,318,887 - -2.4 1,318,913 - Synpcc7942_1294 0.11 +0.0 1,318,918 + Synpcc7942_1294 0.12 -1.1 1,318,921 - Synpcc7942_1294 0.12 +1.0 1,318,944 + Synpcc7942_1294 0.13 -0.2 1,318,945 - Synpcc7942_1294 0.13 +1.0 1,318,952 - Synpcc7942_1294 0.14 -0.5 1,318,952 - Synpcc7942_1294 0.14 -0.4 1,318,990 - Synpcc7942_1294 0.16 +0.6 1,319,001 + Synpcc7942_1294 0.17 -1.4 1,319,031 + Synpcc7942_1294 0.19 -0.7 1,319,038 + Synpcc7942_1294 0.19 -0.2 1,319,040 - Synpcc7942_1294 0.20 +0.4 1,319,104 + Synpcc7942_1294 0.24 -2.7 1,319,208 - Synpcc7942_1294 0.30 +0.7 1,319,208 - Synpcc7942_1294 0.30 -0.1 1,319,222 + Synpcc7942_1294 0.31 +0.7 1,319,271 - Synpcc7942_1294 0.34 -0.4 1,319,289 + Synpcc7942_1294 0.35 +0.0 1,319,289 + Synpcc7942_1294 0.35 +0.3 1,319,289 + Synpcc7942_1294 0.35 +1.0 1,319,297 - Synpcc7942_1294 0.36 -1.0 1,319,309 + Synpcc7942_1294 0.37 +0.8 1,319,313 - Synpcc7942_1294 0.37 +0.3 1,319,320 + Synpcc7942_1294 0.37 +0.0 1,319,334 + Synpcc7942_1294 0.38 -2.2 1,319,342 - Synpcc7942_1294 0.39 -1.4 1,319,408 - Synpcc7942_1294 0.43 +0.0 1,319,414 + Synpcc7942_1294 0.43 +1.0 1,319,415 + Synpcc7942_1294 0.43 +0.5 1,319,422 + Synpcc7942_1294 0.44 -1.3 1,319,422 - Synpcc7942_1294 0.44 -0.5 1,319,501 + Synpcc7942_1294 0.49 +1.0 1,319,501 + Synpcc7942_1294 0.49 -1.2 1,319,512 + Synpcc7942_1294 0.50 -2.8 1,319,522 + Synpcc7942_1294 0.50 -1.0 1,319,522 + Synpcc7942_1294 0.50 +2.0 1,319,522 + Synpcc7942_1294 0.50 -0.5 1,319,527 + Synpcc7942_1294 0.51 +0.2 1,319,530 - Synpcc7942_1294 0.51 -0.7 1,319,530 - Synpcc7942_1294 0.51 -0.2 1,319,530 - Synpcc7942_1294 0.51 -1.8 1,319,530 - Synpcc7942_1294 0.51 -1.3 1,319,530 - +1.0 1,319,530 - Synpcc7942_1294 0.51 +0.1
Or see this region's nucleotide sequence