Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0854 and pixG are separated by 999 nucleotides pixG and pilH/rre7 are separated by 50 nucleotides pilH/rre7 and Synpcc7942_0857 are separated by 19 nucleotides
Synpcc7942_0854: Synpcc7942_0854 - hypothetical protein, at 850,233 to 852,629
_0854
Synpcc7942_0855: pixG - response regulator receiver domain protein (CheY-like), at 853,629 to 854,882
pixG
Synpcc7942_0856: pilH/rre7 - response regulator receiver domain protein (CheY-like), at 854,933 to 855,295
pilH/rre7
Synpcc7942_0857: Synpcc7942_0857 - CheW protein, at 855,315 to 855,821
_0857
Position (kb) 853
854
855 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 852.740 kb on + strand at 852.826 kb on + strand at 852.831 kb on + strand at 852.832 kb on - strand at 852.839 kb on - strand at 852.929 kb on - strand at 853.095 kb on - strand at 853.097 kb on + strand at 853.097 kb on + strand at 853.161 kb on - strand at 853.226 kb on - strand at 853.597 kb on + strand at 853.597 kb on + strand at 853.605 kb on - strand at 853.605 kb on - strand at 853.605 kb on - strand at 853.612 kb on + strand at 853.619 kb on - strand at 853.664 kb on - strand at 853.666 kb on - strand at 853.727 kb on + strand at 853.727 kb on + strand at 853.735 kb on - strand at 853.787 kb on + strand, within pixG at 853.809 kb on + strand, within pixG at 854.005 kb on - strand, within pixG at 854.095 kb on - strand, within pixG at 854.141 kb on - strand, within pixG at 854.251 kb on - strand, within pixG at 854.347 kb on + strand, within pixG at 854.347 kb on + strand, within pixG at 854.355 kb on - strand, within pixG at 854.686 kb on + strand, within pixG at 854.819 kb on - strand at 854.922 kb on - strand at 854.922 kb on - strand at 854.922 kb on - strand at 854.929 kb on + strand at 854.929 kb on + strand at 854.929 kb on + strand at 854.945 kb on - strand at 854.950 kb on - strand at 855.006 kb on + strand, within pilH/rre7 at 855.036 kb on + strand, within pilH/rre7 at 855.046 kb on + strand, within pilH/rre7 at 855.066 kb on + strand, within pilH/rre7 at 855.111 kb on + strand, within pilH/rre7 at 855.210 kb on - strand, within pilH/rre7 at 855.246 kb on - strand, within pilH/rre7 at 855.246 kb on - strand, within pilH/rre7 at 855.284 kb on + strand at 855.309 kb on - strand at 855.309 kb on - strand at 855.325 kb on - strand at 855.454 kb on + strand, within Synpcc7942_0857 at 855.456 kb on + strand, within Synpcc7942_0857 at 855.462 kb on - strand, within Synpcc7942_0857 at 855.462 kb on - strand, within Synpcc7942_0857 at 855.490 kb on + strand, within Synpcc7942_0857 at 855.495 kb on + strand, within Synpcc7942_0857 at 855.495 kb on + strand, within Synpcc7942_0857 at 855.495 kb on + strand, within Synpcc7942_0857 at 855.503 kb on - strand, within Synpcc7942_0857 at 855.503 kb on - strand, within Synpcc7942_0857 at 855.503 kb on - strand, within Synpcc7942_0857 at 855.506 kb on + strand, within Synpcc7942_0857 at 855.548 kb on - strand, within Synpcc7942_0857 at 855.637 kb on - strand, within Synpcc7942_0857 at 855.702 kb on + strand, within Synpcc7942_0857 at 855.708 kb on - strand, within Synpcc7942_0857 at 855.755 kb on - strand, within Synpcc7942_0857 at 855.780 kb on + strand at 855.799 kb on - strand at 855.816 kb on + strand at 855.816 kb on + strand at 855.816 kb on + strand at 855.824 kb on - strand at 855.824 kb on - strand at 855.824 kb on - strand at 855.824 kb on - strand at 855.872 kb on + strand at 855.872 kb on + strand at 855.874 kb on + strand at 855.874 kb on + strand at 855.874 kb on + strand at 855.874 kb on + strand at 855.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 852,740 + -2.8 852,826 + -1.9 852,831 + +1.7 852,832 - +2.8 852,839 - -0.8 852,929 - -1.4 853,095 - -0.6 853,097 + -1.3 853,097 + -1.0 853,161 - -0.4 853,226 - -1.4 853,597 + -0.4 853,597 + +2.2 853,605 - +0.0 853,605 - -0.2 853,605 - -1.0 853,612 + -0.3 853,619 - -0.7 853,664 - -1.5 853,666 - +1.0 853,727 + +0.0 853,727 + +1.7 853,735 - -2.2 853,787 + pixG Synpcc7942_0855 0.13 +2.6 853,809 + pixG Synpcc7942_0855 0.14 -1.8 854,005 - pixG Synpcc7942_0855 0.30 -1.9 854,095 - pixG Synpcc7942_0855 0.37 -1.3 854,141 - pixG Synpcc7942_0855 0.41 +1.6 854,251 - pixG Synpcc7942_0855 0.50 -2.3 854,347 + pixG Synpcc7942_0855 0.57 -0.7 854,347 + pixG Synpcc7942_0855 0.57 -2.1 854,355 - pixG Synpcc7942_0855 0.58 -0.3 854,686 + pixG Synpcc7942_0855 0.84 -0.3 854,819 - -0.7 854,922 - -1.2 854,922 - -0.5 854,922 - +0.0 854,929 + -2.1 854,929 + +0.0 854,929 + -2.0 854,945 - +0.7 854,950 - -1.2 855,006 + pilH/rre7 Synpcc7942_0856 0.20 +1.0 855,036 + pilH/rre7 Synpcc7942_0856 0.28 +3.3 855,046 + pilH/rre7 Synpcc7942_0856 0.31 +1.0 855,066 + pilH/rre7 Synpcc7942_0856 0.37 +3.0 855,111 + pilH/rre7 Synpcc7942_0856 0.49 +1.0 855,210 - pilH/rre7 Synpcc7942_0856 0.76 -1.3 855,246 - pilH/rre7 Synpcc7942_0856 0.86 +0.4 855,246 - pilH/rre7 Synpcc7942_0856 0.86 -0.7 855,284 + +0.4 855,309 - +1.0 855,309 - -0.7 855,325 - -3.2 855,454 + Synpcc7942_0857 0.27 +0.4 855,456 + Synpcc7942_0857 0.28 -0.6 855,462 - Synpcc7942_0857 0.29 -1.1 855,462 - Synpcc7942_0857 0.29 -1.2 855,490 + Synpcc7942_0857 0.35 -1.0 855,495 + Synpcc7942_0857 0.36 -0.8 855,495 + Synpcc7942_0857 0.36 +1.0 855,495 + Synpcc7942_0857 0.36 +1.0 855,503 - Synpcc7942_0857 0.37 +1.0 855,503 - Synpcc7942_0857 0.37 +0.4 855,503 - Synpcc7942_0857 0.37 -0.6 855,506 + Synpcc7942_0857 0.38 -0.2 855,548 - Synpcc7942_0857 0.46 +1.0 855,637 - Synpcc7942_0857 0.64 -0.3 855,702 + Synpcc7942_0857 0.76 -0.1 855,708 - Synpcc7942_0857 0.78 -1.3 855,755 - Synpcc7942_0857 0.87 -1.8 855,780 + +0.2 855,799 - +0.0 855,816 + -1.1 855,816 + +1.9 855,816 + -0.2 855,824 - -2.2 855,824 - -1.0 855,824 - +1.0 855,824 - +0.0 855,872 + -1.2 855,872 + -1.3 855,874 + +1.0 855,874 + -1.3 855,874 + +0.0 855,874 + -1.0 855,882 - +0.1
Or see this region's nucleotide sequence