Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0470 and lacG are separated by 137 nucleotides lacG and Synpcc7942_0472 are separated by 82 nucleotides Synpcc7942_0472 and Synpcc7942_0473 are separated by 76 nucleotides
Synpcc7942_0470: Synpcc7942_0470 - hypothetical protein, at 457,211 to 457,744
_0470
Synpcc7942_0471: lacG - ABC-type sugar transport system permease component-like, at 457,882 to 458,712
lacG
Synpcc7942_0472: Synpcc7942_0472 - Exodeoxyribonuclease VII small subunit, at 458,795 to 459,019
_0472
Synpcc7942_0473: Synpcc7942_0473 - NADH:flavin oxidoreductase Old Yellow Enzyme family-like, at 459,096 to 460,202
_0473
Position (kb) 457
458
459 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 456.959 kb on + strand at 456.959 kb on + strand at 456.966 kb on - strand at 456.967 kb on - strand at 456.967 kb on - strand at 456.967 kb on - strand at 456.969 kb on + strand at 456.974 kb on + strand at 456.977 kb on - strand at 456.982 kb on - strand at 456.982 kb on - strand at 456.992 kb on + strand at 457.044 kb on - strand at 457.060 kb on + strand at 457.136 kb on + strand at 457.137 kb on + strand at 457.159 kb on + strand at 457.162 kb on - strand at 457.164 kb on - strand at 457.167 kb on - strand at 457.186 kb on - strand at 457.205 kb on - strand at 457.260 kb on - strand at 457.299 kb on - strand, within Synpcc7942_0470 at 457.304 kb on + strand, within Synpcc7942_0470 at 457.304 kb on + strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.312 kb on - strand, within Synpcc7942_0470 at 457.346 kb on + strand, within Synpcc7942_0470 at 457.351 kb on + strand, within Synpcc7942_0470 at 457.385 kb on + strand, within Synpcc7942_0470 at 457.393 kb on - strand, within Synpcc7942_0470 at 457.412 kb on + strand at 457.441 kb on + strand at 457.441 kb on + strand at 457.477 kb on - strand, within Synpcc7942_0470 at 457.479 kb on + strand, within Synpcc7942_0470 at 457.482 kb on - strand, within Synpcc7942_0470 at 457.484 kb on + strand, within Synpcc7942_0470 at 457.490 kb on + strand, within Synpcc7942_0470 at 457.492 kb on - strand, within Synpcc7942_0470 at 457.492 kb on - strand at 457.508 kb on - strand, within Synpcc7942_0470 at 457.518 kb on + strand, within Synpcc7942_0470 at 457.525 kb on - strand, within Synpcc7942_0470 at 457.553 kb on - strand, within Synpcc7942_0470 at 457.572 kb on + strand, within Synpcc7942_0470 at 457.580 kb on - strand, within Synpcc7942_0470 at 457.582 kb on + strand, within Synpcc7942_0470 at 457.598 kb on + strand, within Synpcc7942_0470 at 457.605 kb on - strand, within Synpcc7942_0470 at 457.605 kb on - strand, within Synpcc7942_0470 at 457.608 kb on + strand, within Synpcc7942_0470 at 457.616 kb on - strand, within Synpcc7942_0470 at 457.647 kb on - strand, within Synpcc7942_0470 at 457.655 kb on + strand, within Synpcc7942_0470 at 457.692 kb on - strand at 457.692 kb on - strand at 457.757 kb on - strand at 457.780 kb on - strand at 457.785 kb on + strand at 457.785 kb on + strand at 457.793 kb on - strand at 457.803 kb on - strand at 457.843 kb on + strand at 457.870 kb on + strand at 457.880 kb on - strand at 457.884 kb on + strand at 457.887 kb on - strand at 457.920 kb on - strand at 457.922 kb on - strand at 457.922 kb on - strand at 457.934 kb on + strand at 457.989 kb on - strand, within lacG at 457.989 kb on - strand, within lacG at 458.005 kb on - strand, within lacG at 458.086 kb on + strand, within lacG at 458.094 kb on - strand, within lacG at 458.124 kb on - strand, within lacG at 458.137 kb on + strand, within lacG at 458.145 kb on + strand, within lacG at 458.161 kb on + strand, within lacG at 458.169 kb on - strand, within lacG at 458.169 kb on - strand, within lacG at 458.254 kb on + strand, within lacG at 458.262 kb on + strand, within lacG at 458.264 kb on + strand, within lacG at 458.264 kb on + strand, within lacG at 458.274 kb on - strand, within lacG at 458.279 kb on + strand, within lacG at 458.279 kb on + strand, within lacG at 458.289 kb on + strand, within lacG at 458.294 kb on + strand, within lacG at 458.294 kb on + strand, within lacG at 458.297 kb on - strand, within lacG at 458.302 kb on - strand, within lacG at 458.424 kb on - strand, within lacG at 458.483 kb on - strand, within lacG at 458.490 kb on + strand, within lacG at 458.545 kb on + strand, within lacG at 458.550 kb on + strand, within lacG at 458.551 kb on + strand, within lacG at 458.551 kb on + strand, within lacG at 458.559 kb on - strand, within lacG at 458.572 kb on + strand, within lacG at 458.625 kb on - strand, within lacG at 458.626 kb on - strand, within lacG at 458.667 kb on + strand at 458.667 kb on + strand at 458.667 kb on + strand at 458.667 kb on + strand at 458.675 kb on - strand at 458.706 kb on - strand at 458.707 kb on + strand at 458.714 kb on - strand at 458.752 kb on + strand at 458.767 kb on + strand at 458.772 kb on + strand at 458.803 kb on + strand at 458.803 kb on + strand at 458.811 kb on - strand at 458.861 kb on + strand, within Synpcc7942_0472 at 458.871 kb on + strand, within Synpcc7942_0472 at 458.879 kb on - strand, within Synpcc7942_0472 at 458.881 kb on + strand, within Synpcc7942_0472 at 458.889 kb on - strand, within Synpcc7942_0472 at 458.889 kb on - strand, within Synpcc7942_0472 at 458.974 kb on - strand, within Synpcc7942_0472 at 459.033 kb on + strand at 459.086 kb on + strand at 459.094 kb on - strand at 459.094 kb on - strand at 459.126 kb on + strand at 459.126 kb on + strand at 459.139 kb on + strand at 459.162 kb on + strand at 459.171 kb on + strand at 459.203 kb on - strand at 459.217 kb on + strand, within Synpcc7942_0473 at 459.225 kb on - strand, within Synpcc7942_0473 at 459.245 kb on + strand, within Synpcc7942_0473 at 459.281 kb on + strand, within Synpcc7942_0473 at 459.294 kb on + strand, within Synpcc7942_0473 at 459.297 kb on - strand, within Synpcc7942_0473 at 459.302 kb on - strand, within Synpcc7942_0473 at 459.302 kb on - strand, within Synpcc7942_0473 at 459.302 kb on - strand, within Synpcc7942_0473 at 459.313 kb on + strand, within Synpcc7942_0473 at 459.313 kb on + strand, within Synpcc7942_0473 at 459.315 kb on - strand, within Synpcc7942_0473 at 459.328 kb on + strand, within Synpcc7942_0473 at 459.328 kb on + strand, within Synpcc7942_0473 at 459.330 kb on + strand, within Synpcc7942_0473 at 459.336 kb on - strand, within Synpcc7942_0473 at 459.345 kb on - strand, within Synpcc7942_0473 at 459.346 kb on - strand, within Synpcc7942_0473 at 459.346 kb on - strand, within Synpcc7942_0473 at 459.375 kb on - strand, within Synpcc7942_0473 at 459.401 kb on + strand, within Synpcc7942_0473 at 459.445 kb on + strand, within Synpcc7942_0473 at 459.448 kb on + strand, within Synpcc7942_0473 at 459.455 kb on + strand, within Synpcc7942_0473 at 459.470 kb on - strand, within Synpcc7942_0473 at 459.491 kb on + strand, within Synpcc7942_0473 at 459.492 kb on + strand at 459.500 kb on + strand, within Synpcc7942_0473 at 459.500 kb on + strand, within Synpcc7942_0473 at 459.505 kb on + strand, within Synpcc7942_0473 at 459.523 kb on - strand, within Synpcc7942_0473 at 459.524 kb on - strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.564 kb on + strand, within Synpcc7942_0473 at 459.578 kb on - strand at 459.624 kb on - strand, within Synpcc7942_0473 at 459.624 kb on - strand, within Synpcc7942_0473 at 459.626 kb on - strand, within Synpcc7942_0473 at 459.626 kb on - strand, within Synpcc7942_0473 at 459.633 kb on + strand, within Synpcc7942_0473 at 459.665 kb on + strand, within Synpcc7942_0473 at 459.670 kb on + strand, within Synpcc7942_0473 at 459.670 kb on + strand, within Synpcc7942_0473 at 459.683 kb on - strand, within Synpcc7942_0473 at 459.689 kb on + strand, within Synpcc7942_0473 at 459.704 kb on - strand, within Synpcc7942_0473
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 456,959 + -2.2 456,959 + -0.3 456,966 - -1.3 456,967 - -0.4 456,967 - -0.6 456,967 - +0.1 456,969 + -0.3 456,974 + +1.0 456,977 - -0.0 456,982 - -0.5 456,982 - -0.1 456,992 + -1.4 457,044 - +1.0 457,060 + -1.8 457,136 + +0.1 457,137 + -0.4 457,159 + -0.0 457,162 - -2.5 457,164 - +0.4 457,167 - +2.3 457,186 - -0.0 457,205 - -1.2 457,260 - +2.6 457,299 - Synpcc7942_0470 0.16 -1.3 457,304 + Synpcc7942_0470 0.17 -0.6 457,304 + Synpcc7942_0470 0.17 +2.8 457,312 - Synpcc7942_0470 0.19 +0.9 457,312 - Synpcc7942_0470 0.19 -1.2 457,312 - Synpcc7942_0470 0.19 +2.5 457,312 - Synpcc7942_0470 0.19 +0.2 457,312 - Synpcc7942_0470 0.19 -1.2 457,346 + Synpcc7942_0470 0.25 -0.9 457,351 + Synpcc7942_0470 0.26 -0.7 457,385 + Synpcc7942_0470 0.33 -1.0 457,393 - Synpcc7942_0470 0.34 +1.0 457,412 + -1.5 457,441 + -1.6 457,441 + +1.0 457,477 - Synpcc7942_0470 0.50 -2.1 457,479 + Synpcc7942_0470 0.50 -0.8 457,482 - Synpcc7942_0470 0.51 -1.7 457,484 + Synpcc7942_0470 0.51 -0.7 457,490 + Synpcc7942_0470 0.52 +0.5 457,492 - Synpcc7942_0470 0.53 -1.8 457,492 - +1.0 457,508 - Synpcc7942_0470 0.56 +2.3 457,518 + Synpcc7942_0470 0.57 -1.3 457,525 - Synpcc7942_0470 0.59 -1.0 457,553 - Synpcc7942_0470 0.64 -1.0 457,572 + Synpcc7942_0470 0.68 +0.6 457,580 - Synpcc7942_0470 0.69 -0.0 457,582 + Synpcc7942_0470 0.69 -1.0 457,598 + Synpcc7942_0470 0.72 +1.8 457,605 - Synpcc7942_0470 0.74 -0.6 457,605 - Synpcc7942_0470 0.74 +1.4 457,608 + Synpcc7942_0470 0.74 -0.0 457,616 - Synpcc7942_0470 0.76 -0.5 457,647 - Synpcc7942_0470 0.82 -1.0 457,655 + Synpcc7942_0470 0.83 -2.5 457,692 - -1.6 457,692 - -1.0 457,757 - +1.0 457,780 - -1.2 457,785 + -0.5 457,785 + +1.9 457,793 - +1.0 457,803 - -2.4 457,843 + -2.0 457,870 + +1.5 457,880 - -2.4 457,884 + -0.1 457,887 - +0.8 457,920 - -0.3 457,922 - +2.0 457,922 - +1.0 457,934 + +1.0 457,989 - lacG Synpcc7942_0471 0.13 +0.2 457,989 - lacG Synpcc7942_0471 0.13 -2.2 458,005 - lacG Synpcc7942_0471 0.15 -1.3 458,086 + lacG Synpcc7942_0471 0.25 -2.3 458,094 - lacG Synpcc7942_0471 0.26 +1.0 458,124 - lacG Synpcc7942_0471 0.29 -0.6 458,137 + lacG Synpcc7942_0471 0.31 -0.8 458,145 + lacG Synpcc7942_0471 0.32 -1.3 458,161 + lacG Synpcc7942_0471 0.34 -1.0 458,169 - lacG Synpcc7942_0471 0.35 -0.2 458,169 - lacG Synpcc7942_0471 0.35 -0.0 458,254 + lacG Synpcc7942_0471 0.45 -0.0 458,262 + lacG Synpcc7942_0471 0.46 -1.6 458,264 + lacG Synpcc7942_0471 0.46 -0.4 458,264 + lacG Synpcc7942_0471 0.46 -0.0 458,274 - lacG Synpcc7942_0471 0.47 +1.0 458,279 + lacG Synpcc7942_0471 0.48 -1.8 458,279 + lacG Synpcc7942_0471 0.48 +2.0 458,289 + lacG Synpcc7942_0471 0.49 -0.2 458,294 + lacG Synpcc7942_0471 0.50 -0.9 458,294 + lacG Synpcc7942_0471 0.50 -0.0 458,297 - lacG Synpcc7942_0471 0.50 +1.0 458,302 - lacG Synpcc7942_0471 0.51 -0.0 458,424 - lacG Synpcc7942_0471 0.65 -0.6 458,483 - lacG Synpcc7942_0471 0.72 -0.0 458,490 + lacG Synpcc7942_0471 0.73 +2.0 458,545 + lacG Synpcc7942_0471 0.80 -2.0 458,550 + lacG Synpcc7942_0471 0.80 +0.7 458,551 + lacG Synpcc7942_0471 0.81 -0.0 458,551 + lacG Synpcc7942_0471 0.81 +1.0 458,559 - lacG Synpcc7942_0471 0.81 +0.3 458,572 + lacG Synpcc7942_0471 0.83 -0.0 458,625 - lacG Synpcc7942_0471 0.89 -0.8 458,626 - lacG Synpcc7942_0471 0.90 -0.6 458,667 + -0.6 458,667 + -0.6 458,667 + +0.4 458,667 + -1.6 458,675 - -1.6 458,706 - -0.6 458,707 + -1.2 458,714 - -0.3 458,752 + -0.4 458,767 + -1.9 458,772 + +2.0 458,803 + -0.4 458,803 + -0.1 458,811 - -0.6 458,861 + Synpcc7942_0472 0.29 +2.0 458,871 + Synpcc7942_0472 0.34 -1.1 458,879 - Synpcc7942_0472 0.37 +2.2 458,881 + Synpcc7942_0472 0.38 -0.4 458,889 - Synpcc7942_0472 0.42 -3.0 458,889 - Synpcc7942_0472 0.42 -2.1 458,974 - Synpcc7942_0472 0.80 +1.3 459,033 + -0.1 459,086 + -1.0 459,094 - -1.3 459,094 - -0.7 459,126 + -0.5 459,126 + +0.5 459,139 + -0.9 459,162 + +0.3 459,171 + +2.6 459,203 - -0.4 459,217 + Synpcc7942_0473 0.11 -0.0 459,225 - Synpcc7942_0473 0.12 +0.2 459,245 + Synpcc7942_0473 0.13 -1.0 459,281 + Synpcc7942_0473 0.17 -0.5 459,294 + Synpcc7942_0473 0.18 -0.0 459,297 - Synpcc7942_0473 0.18 -0.3 459,302 - Synpcc7942_0473 0.19 -2.0 459,302 - Synpcc7942_0473 0.19 +0.3 459,302 - Synpcc7942_0473 0.19 +1.4 459,313 + Synpcc7942_0473 0.20 +0.6 459,313 + Synpcc7942_0473 0.20 -0.6 459,315 - Synpcc7942_0473 0.20 -0.2 459,328 + Synpcc7942_0473 0.21 -0.2 459,328 + Synpcc7942_0473 0.21 -2.5 459,330 + Synpcc7942_0473 0.21 -0.3 459,336 - Synpcc7942_0473 0.22 +0.5 459,345 - Synpcc7942_0473 0.22 -0.7 459,346 - Synpcc7942_0473 0.23 +1.4 459,346 - Synpcc7942_0473 0.23 +1.0 459,375 - Synpcc7942_0473 0.25 +1.0 459,401 + Synpcc7942_0473 0.28 +0.7 459,445 + Synpcc7942_0473 0.32 +1.2 459,448 + Synpcc7942_0473 0.32 -0.8 459,455 + Synpcc7942_0473 0.32 -0.7 459,470 - Synpcc7942_0473 0.34 -0.8 459,491 + Synpcc7942_0473 0.36 -0.3 459,492 + -2.1 459,500 + Synpcc7942_0473 0.36 -0.1 459,500 + Synpcc7942_0473 0.36 -3.7 459,505 + Synpcc7942_0473 0.37 -1.1 459,523 - Synpcc7942_0473 0.39 +1.4 459,524 - Synpcc7942_0473 0.39 -1.0 459,564 + Synpcc7942_0473 0.42 -0.8 459,564 + Synpcc7942_0473 0.42 -1.0 459,564 + Synpcc7942_0473 0.42 +0.1 459,564 + Synpcc7942_0473 0.42 +1.0 459,578 - -2.0 459,624 - Synpcc7942_0473 0.48 +0.4 459,624 - Synpcc7942_0473 0.48 -2.2 459,626 - Synpcc7942_0473 0.48 +1.0 459,626 - Synpcc7942_0473 0.48 -0.9 459,633 + Synpcc7942_0473 0.49 -1.0 459,665 + Synpcc7942_0473 0.51 -0.9 459,670 + Synpcc7942_0473 0.52 -1.8 459,670 + Synpcc7942_0473 0.52 -0.6 459,683 - Synpcc7942_0473 0.53 -0.0 459,689 + Synpcc7942_0473 0.54 -2.1 459,704 - Synpcc7942_0473 0.55 -0.3
Or see this region's nucleotide sequence