Experiment: Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispE and ksgA overlap by 4 nucleotides ksgA and Synpcc7942_0312 are separated by 26 nucleotides Synpcc7942_0312 and Synpcc7942_0313 are separated by 53 nucleotides
Synpcc7942_0310: ispE - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 309,473 to 310,417
ispE
Synpcc7942_0311: ksgA - dimethyladenosine transferase, at 310,414 to 311,253
ksgA
Synpcc7942_0312: Synpcc7942_0312 - hypothetical protein, at 311,280 to 311,708
_0312
Synpcc7942_0313: Synpcc7942_0313 - hypothetical protein, at 311,762 to 312,274
_0313
Position (kb) 310
311
312 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 309.439 kb on + strand at 309.486 kb on - strand at 309.489 kb on - strand at 310.455 kb on + strand at 310.455 kb on + strand at 310.479 kb on + strand at 310.479 kb on + strand at 310.519 kb on + strand, within ksgA at 310.553 kb on + strand at 310.555 kb on + strand, within ksgA at 310.555 kb on + strand, within ksgA at 310.561 kb on - strand, within ksgA at 310.563 kb on - strand, within ksgA at 310.563 kb on - strand at 310.568 kb on - strand, within ksgA at 310.573 kb on - strand at 310.632 kb on + strand, within ksgA at 310.632 kb on + strand, within ksgA at 310.640 kb on - strand at 310.650 kb on + strand at 310.662 kb on + strand, within ksgA at 310.694 kb on + strand, within ksgA at 310.722 kb on - strand, within ksgA at 310.738 kb on - strand at 310.750 kb on + strand, within ksgA at 310.751 kb on + strand, within ksgA at 310.751 kb on + strand at 310.794 kb on + strand, within ksgA at 310.800 kb on + strand at 310.802 kb on - strand, within ksgA at 310.802 kb on - strand at 310.916 kb on - strand, within ksgA at 310.951 kb on - strand, within ksgA at 311.009 kb on - strand at 311.034 kb on - strand, within ksgA at 311.103 kb on - strand, within ksgA at 311.135 kb on - strand, within ksgA at 311.210 kb on + strand at 311.215 kb on - strand at 311.226 kb on - strand at 311.238 kb on - strand at 311.244 kb on - strand at 311.260 kb on + strand at 311.263 kb on - strand at 311.263 kb on - strand at 311.263 kb on - strand at 311.301 kb on + strand at 311.301 kb on + strand at 311.307 kb on - strand at 311.307 kb on - strand at 311.308 kb on + strand at 311.330 kb on + strand, within Synpcc7942_0312 at 311.330 kb on + strand, within Synpcc7942_0312 at 311.330 kb on - strand, within Synpcc7942_0312 at 311.338 kb on - strand, within Synpcc7942_0312 at 311.338 kb on - strand, within Synpcc7942_0312 at 311.340 kb on + strand, within Synpcc7942_0312 at 311.348 kb on - strand, within Synpcc7942_0312 at 311.348 kb on - strand, within Synpcc7942_0312 at 311.369 kb on + strand at 311.374 kb on + strand, within Synpcc7942_0312 at 311.380 kb on + strand, within Synpcc7942_0312 at 311.384 kb on - strand, within Synpcc7942_0312 at 311.397 kb on + strand, within Synpcc7942_0312 at 311.416 kb on - strand, within Synpcc7942_0312 at 311.429 kb on - strand, within Synpcc7942_0312 at 311.438 kb on + strand, within Synpcc7942_0312 at 311.438 kb on + strand, within Synpcc7942_0312 at 311.471 kb on + strand, within Synpcc7942_0312 at 311.471 kb on + strand, within Synpcc7942_0312 at 311.479 kb on - strand, within Synpcc7942_0312 at 311.492 kb on - strand, within Synpcc7942_0312 at 311.498 kb on - strand, within Synpcc7942_0312 at 311.513 kb on - strand, within Synpcc7942_0312 at 311.519 kb on + strand, within Synpcc7942_0312 at 311.527 kb on - strand, within Synpcc7942_0312 at 311.552 kb on + strand, within Synpcc7942_0312 at 311.571 kb on + strand, within Synpcc7942_0312 at 311.588 kb on + strand, within Synpcc7942_0312 at 311.611 kb on + strand, within Synpcc7942_0312 at 311.621 kb on + strand, within Synpcc7942_0312 at 311.661 kb on + strand, within Synpcc7942_0312 at 311.667 kb on + strand at 311.718 kb on + strand at 311.719 kb on - strand at 311.726 kb on + strand at 311.733 kb on + strand at 311.741 kb on - strand at 311.763 kb on - strand at 311.795 kb on - strand at 311.811 kb on + strand at 311.839 kb on + strand, within Synpcc7942_0313 at 311.839 kb on + strand, within Synpcc7942_0313 at 311.842 kb on - strand, within Synpcc7942_0313 at 311.850 kb on + strand, within Synpcc7942_0313 at 311.924 kb on - strand, within Synpcc7942_0313 at 311.974 kb on - strand, within Synpcc7942_0313 at 311.981 kb on + strand, within Synpcc7942_0313 at 311.985 kb on + strand, within Synpcc7942_0313 at 312.019 kb on - strand, within Synpcc7942_0313 at 312.036 kb on + strand, within Synpcc7942_0313 at 312.036 kb on + strand, within Synpcc7942_0313 at 312.044 kb on - strand, within Synpcc7942_0313 at 312.049 kb on - strand, within Synpcc7942_0313 at 312.054 kb on - strand, within Synpcc7942_0313 at 312.060 kb on - strand, within Synpcc7942_0313 at 312.076 kb on + strand, within Synpcc7942_0313 at 312.081 kb on + strand, within Synpcc7942_0313 at 312.084 kb on - strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.156 kb on - strand, within Synpcc7942_0313 at 312.173 kb on + strand, within Synpcc7942_0313
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in Fresh BG11R in Test Tubes with Spin rep 1 remove 309,439 + +1.0 309,486 - -0.6 309,489 - +1.0 310,455 + -1.0 310,455 + -1.1 310,479 + -2.6 310,479 + -0.4 310,519 + ksgA Synpcc7942_0311 0.12 -0.6 310,553 + -1.4 310,555 + ksgA Synpcc7942_0311 0.17 -1.8 310,555 + ksgA Synpcc7942_0311 0.17 -2.4 310,561 - ksgA Synpcc7942_0311 0.17 -0.6 310,563 - ksgA Synpcc7942_0311 0.18 -1.8 310,563 - +1.0 310,568 - ksgA Synpcc7942_0311 0.18 +1.0 310,573 - -0.6 310,632 + ksgA Synpcc7942_0311 0.26 -0.0 310,632 + ksgA Synpcc7942_0311 0.26 -2.9 310,640 - +1.0 310,650 + -0.0 310,662 + ksgA Synpcc7942_0311 0.30 -1.6 310,694 + ksgA Synpcc7942_0311 0.33 -1.4 310,722 - ksgA Synpcc7942_0311 0.37 -0.6 310,738 - -1.2 310,750 + ksgA Synpcc7942_0311 0.40 -1.8 310,751 + ksgA Synpcc7942_0311 0.40 +1.2 310,751 + -1.8 310,794 + ksgA Synpcc7942_0311 0.45 -1.4 310,800 + -2.0 310,802 - ksgA Synpcc7942_0311 0.46 -3.9 310,802 - -2.4 310,916 - ksgA Synpcc7942_0311 0.60 -1.4 310,951 - ksgA Synpcc7942_0311 0.64 -2.0 311,009 - -1.0 311,034 - ksgA Synpcc7942_0311 0.74 -1.0 311,103 - ksgA Synpcc7942_0311 0.82 -0.8 311,135 - ksgA Synpcc7942_0311 0.86 -0.6 311,210 + -0.0 311,215 - -0.0 311,226 - -0.6 311,238 - +3.3 311,244 - -1.0 311,260 + -2.6 311,263 - -1.4 311,263 - -0.6 311,263 - -2.9 311,301 + +1.0 311,301 + +1.6 311,307 - -1.5 311,307 - -0.9 311,308 + +0.2 311,330 + Synpcc7942_0312 0.12 +2.0 311,330 + Synpcc7942_0312 0.12 +1.0 311,330 - Synpcc7942_0312 0.12 +1.0 311,338 - Synpcc7942_0312 0.14 -0.0 311,338 - Synpcc7942_0312 0.14 +1.0 311,340 + Synpcc7942_0312 0.14 -0.6 311,348 - Synpcc7942_0312 0.16 -1.2 311,348 - Synpcc7942_0312 0.16 +1.0 311,369 + -1.2 311,374 + Synpcc7942_0312 0.22 +0.6 311,380 + Synpcc7942_0312 0.23 -0.6 311,384 - Synpcc7942_0312 0.24 -1.0 311,397 + Synpcc7942_0312 0.27 +1.0 311,416 - Synpcc7942_0312 0.32 -0.0 311,429 - Synpcc7942_0312 0.35 -0.5 311,438 + Synpcc7942_0312 0.37 -1.0 311,438 + Synpcc7942_0312 0.37 +1.0 311,471 + Synpcc7942_0312 0.45 +0.3 311,471 + Synpcc7942_0312 0.45 -0.5 311,479 - Synpcc7942_0312 0.46 -1.0 311,492 - Synpcc7942_0312 0.49 +2.0 311,498 - Synpcc7942_0312 0.51 -0.8 311,513 - Synpcc7942_0312 0.54 -1.2 311,519 + Synpcc7942_0312 0.56 +0.0 311,527 - Synpcc7942_0312 0.58 -0.8 311,552 + Synpcc7942_0312 0.63 -0.9 311,571 + Synpcc7942_0312 0.68 -1.0 311,588 + Synpcc7942_0312 0.72 -2.0 311,611 + Synpcc7942_0312 0.77 +1.0 311,621 + Synpcc7942_0312 0.79 -0.4 311,661 + Synpcc7942_0312 0.89 -1.2 311,667 + -2.0 311,718 + -1.1 311,719 - -1.2 311,726 + -0.6 311,733 + +1.3 311,741 - -1.4 311,763 - -0.3 311,795 - -1.0 311,811 + -0.0 311,839 + Synpcc7942_0313 0.15 +0.4 311,839 + Synpcc7942_0313 0.15 -1.5 311,842 - Synpcc7942_0313 0.16 -2.2 311,850 + Synpcc7942_0313 0.17 -0.5 311,924 - Synpcc7942_0313 0.32 -1.6 311,974 - Synpcc7942_0313 0.41 -0.2 311,981 + Synpcc7942_0313 0.43 +1.0 311,985 + Synpcc7942_0313 0.43 -2.0 312,019 - Synpcc7942_0313 0.50 +0.7 312,036 + Synpcc7942_0313 0.53 -0.8 312,036 + Synpcc7942_0313 0.53 -1.1 312,044 - Synpcc7942_0313 0.55 +0.7 312,049 - Synpcc7942_0313 0.56 -0.0 312,054 - Synpcc7942_0313 0.57 -0.9 312,060 - Synpcc7942_0313 0.58 +0.5 312,076 + Synpcc7942_0313 0.61 +0.2 312,081 + Synpcc7942_0313 0.62 -0.0 312,084 - Synpcc7942_0313 0.63 -0.2 312,133 + Synpcc7942_0313 0.72 +2.5 312,133 + Synpcc7942_0313 0.72 -2.8 312,133 + Synpcc7942_0313 0.72 +1.0 312,133 + Synpcc7942_0313 0.72 +0.2 312,141 - Synpcc7942_0313 0.74 -0.3 312,141 - Synpcc7942_0313 0.74 +2.0 312,141 - Synpcc7942_0313 0.74 +0.4 312,141 - Synpcc7942_0313 0.74 -1.2 312,141 - Synpcc7942_0313 0.74 -0.6 312,141 - Synpcc7942_0313 0.74 +3.3 312,156 - Synpcc7942_0313 0.77 +1.0 312,173 + Synpcc7942_0313 0.80 +0.0
Or see this region's nucleotide sequence