Experiment: H2 McCas with 20 minute oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06625 and MMP_RS06630 are separated by 33 nucleotides MMP_RS06630 and MMP_RS06635 are separated by 39 nucleotides MMP_RS06635 and MMP_RS06640 are separated by 62 nucleotides MMP_RS06640 and MMP_RS06645 are separated by 125 nucleotides
MMP_RS06625: MMP_RS06625 - DNA primase, at 1,269,354 to 1,270,664
_RS06625
MMP_RS06630: MMP_RS06630 - UPF0058 family protein, at 1,270,698 to 1,270,991
_RS06630
MMP_RS06635: MMP_RS06635 - UbiD family decarboxylase, at 1,271,031 to 1,272,308
_RS06635
MMP_RS06640: MMP_RS06640 - 50S ribosomal protein L16, at 1,272,371 to 1,272,892
_RS06640
MMP_RS06645: MMP_RS06645 - GTP-binding protein EngB, at 1,273,018 to 1,273,710
_RS06645
Position (kb)
1271
1272
1273 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1270.408 kb on - strand, within MMP_RS06625 at 1270.409 kb on + strand, within MMP_RS06625 at 1270.817 kb on + strand, within MMP_RS06630 at 1270.873 kb on - strand, within MMP_RS06630 at 1271.352 kb on - strand at 1271.381 kb on - strand at 1271.392 kb on - strand at 1271.508 kb on - strand at 1271.650 kb on - strand at 1271.780 kb on + strand at 1271.885 kb on + strand at 1271.885 kb on + strand at 1272.152 kb on - strand at 1272.325 kb on + strand at 1272.380 kb on + strand at 1272.674 kb on - strand at 1272.953 kb on + strand at 1273.058 kb on + strand at 1273.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 20 minute oxygen exposure remove 1,270,408 - MMP_RS06625 0.80 -1.1 1,270,409 + MMP_RS06625 0.80 -3.0 1,270,817 + MMP_RS06630 0.40 -3.7 1,270,873 - MMP_RS06630 0.60 -4.2 1,271,352 - -1.9 1,271,381 - -2.3 1,271,392 - -2.6 1,271,508 - -1.5 1,271,650 - -2.6 1,271,780 + -2.4 1,271,885 + -2.7 1,271,885 + -1.5 1,272,152 - -1.4 1,272,325 + -0.2 1,272,380 + +0.5 1,272,674 - -1.9 1,272,953 + -1.6 1,273,058 + -0.7 1,273,250 + -1.8
Or see this region's nucleotide sequence