Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26220 and BWI76_RS26225 are separated by 358 nucleotides BWI76_RS26225 and BWI76_RS26230 overlap by 4 nucleotides
BWI76_RS26220: BWI76_RS26220 - aspartate-semialdehyde dehydrogenase, at 5,426,671 to 5,427,777
_RS26220
BWI76_RS26225: BWI76_RS26225 - DNA processing protein DprA, at 5,428,136 to 5,429,341
_RS26225
BWI76_RS26230: BWI76_RS26230 - ATP-dependent DNA helicase, at 5,429,338 to 5,431,437
_RS26230
Position (kb) 5428
5429
5430 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 5427.801 kb on - strand at 5427.826 kb on - strand at 5427.836 kb on - strand at 5428.144 kb on + strand at 5428.152 kb on - strand at 5428.152 kb on - strand at 5428.184 kb on + strand at 5428.200 kb on - strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.206 kb on + strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.214 kb on - strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.228 kb on + strand at 5428.236 kb on - strand at 5428.388 kb on + strand, within BWI76_RS26225 at 5428.407 kb on + strand, within BWI76_RS26225 at 5428.530 kb on - strand, within BWI76_RS26225 at 5428.569 kb on - strand, within BWI76_RS26225 at 5428.714 kb on - strand, within BWI76_RS26225 at 5428.822 kb on + strand, within BWI76_RS26225 at 5428.822 kb on + strand, within BWI76_RS26225 at 5428.944 kb on - strand, within BWI76_RS26225 at 5429.056 kb on + strand, within BWI76_RS26225 at 5429.056 kb on + strand, within BWI76_RS26225 at 5429.062 kb on + strand, within BWI76_RS26225 at 5429.098 kb on + strand, within BWI76_RS26225 at 5429.179 kb on + strand, within BWI76_RS26225 at 5429.276 kb on + strand at 5429.284 kb on - strand at 5429.479 kb on - strand at 5429.644 kb on - strand, within BWI76_RS26230 at 5430.001 kb on - strand, within BWI76_RS26230 at 5430.017 kb on + strand, within BWI76_RS26230 at 5430.031 kb on - strand, within BWI76_RS26230 at 5430.063 kb on + strand, within BWI76_RS26230 at 5430.071 kb on + strand, within BWI76_RS26230 at 5430.071 kb on + strand, within BWI76_RS26230 at 5430.079 kb on - strand, within BWI76_RS26230 at 5430.079 kb on - strand, within BWI76_RS26230 at 5430.159 kb on - strand, within BWI76_RS26230 at 5430.247 kb on + strand, within BWI76_RS26230 at 5430.249 kb on + strand, within BWI76_RS26230 at 5430.255 kb on - strand, within BWI76_RS26230 at 5430.287 kb on - strand, within BWI76_RS26230 at 5430.315 kb on + strand, within BWI76_RS26230
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 5,427,801 - -1.7 5,427,826 - -1.2 5,427,836 - -0.9 5,428,144 + +0.1 5,428,152 - -0.1 5,428,152 - +0.1 5,428,184 + +1.5 5,428,200 - -0.8 5,428,206 + +0.2 5,428,206 + +0.4 5,428,206 + -0.5 5,428,206 + -1.2 5,428,206 + -0.2 5,428,206 + -0.1 5,428,206 + +0.1 5,428,206 + -1.1 5,428,214 - +0.1 5,428,214 - -0.4 5,428,214 - +0.2 5,428,214 - -1.0 5,428,214 - -0.5 5,428,214 - -0.8 5,428,228 + -0.4 5,428,228 + -0.6 5,428,228 + -0.1 5,428,228 + -1.5 5,428,228 + +0.3 5,428,228 + -1.2 5,428,236 - +0.0 5,428,388 + BWI76_RS26225 0.21 -0.6 5,428,407 + BWI76_RS26225 0.22 +0.5 5,428,530 - BWI76_RS26225 0.33 -0.0 5,428,569 - BWI76_RS26225 0.36 +0.4 5,428,714 - BWI76_RS26225 0.48 +0.1 5,428,822 + BWI76_RS26225 0.57 -1.5 5,428,822 + BWI76_RS26225 0.57 +4.3 5,428,944 - BWI76_RS26225 0.67 -0.4 5,429,056 + BWI76_RS26225 0.76 +2.4 5,429,056 + BWI76_RS26225 0.76 -0.6 5,429,062 + BWI76_RS26225 0.77 -0.2 5,429,098 + BWI76_RS26225 0.80 -0.5 5,429,179 + BWI76_RS26225 0.86 -0.8 5,429,276 + +0.1 5,429,284 - +0.2 5,429,479 - -1.0 5,429,644 - BWI76_RS26230 0.15 +0.0 5,430,001 - BWI76_RS26230 0.32 -0.3 5,430,017 + BWI76_RS26230 0.32 +0.4 5,430,031 - BWI76_RS26230 0.33 +0.1 5,430,063 + BWI76_RS26230 0.35 -0.5 5,430,071 + BWI76_RS26230 0.35 -0.1 5,430,071 + BWI76_RS26230 0.35 -0.6 5,430,079 - BWI76_RS26230 0.35 -1.7 5,430,079 - BWI76_RS26230 0.35 +0.3 5,430,159 - BWI76_RS26230 0.39 -0.1 5,430,247 + BWI76_RS26230 0.43 -0.1 5,430,249 + BWI76_RS26230 0.43 +0.9 5,430,255 - BWI76_RS26230 0.44 -0.1 5,430,287 - BWI76_RS26230 0.45 +0.9 5,430,315 + BWI76_RS26230 0.47 -0.1
Or see this region's nucleotide sequence