Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23235 and BWI76_RS23240 are separated by 128 nucleotides BWI76_RS23240 and BWI76_RS23245 are separated by 12 nucleotides BWI76_RS23245 and BWI76_RS23250 overlap by 16 nucleotides BWI76_RS23250 and BWI76_RS23255 overlap by 4 nucleotides
BWI76_RS23235: BWI76_RS23235 - pitrilysin, at 4,843,827 to 4,846,712
_RS23235
BWI76_RS23240: BWI76_RS23240 - exonuclease V subunit gamma, at 4,846,841 to 4,850,215
_RS23240
BWI76_RS23245: BWI76_RS23245 - hypothetical protein, at 4,850,228 to 4,850,551
_RS23245
BWI76_RS23250: BWI76_RS23250 - hypothetical protein, at 4,850,536 to 4,850,943
_RS23250
BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503
_RS23255
Position (kb)
4846
4847
4848
4849
4850
4851 Strain fitness (log2 ratio)
-2
-1
0
1 at 4846.713 kb on + strand at 4847.810 kb on + strand, within BWI76_RS23240 at 4848.002 kb on - strand, within BWI76_RS23240 at 4848.397 kb on + strand, within BWI76_RS23240 at 4848.798 kb on + strand, within BWI76_RS23240 at 4848.932 kb on + strand, within BWI76_RS23240 at 4848.940 kb on - strand, within BWI76_RS23240 at 4849.704 kb on + strand, within BWI76_RS23240 at 4849.712 kb on - strand, within BWI76_RS23240 at 4850.375 kb on - strand, within BWI76_RS23245 at 4850.433 kb on + strand, within BWI76_RS23245 at 4850.679 kb on - strand, within BWI76_RS23250 at 4850.784 kb on - strand, within BWI76_RS23250 at 4850.953 kb on - strand at 4851.173 kb on + strand, within BWI76_RS23255
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 4,846,713 + -0.6 4,847,810 + BWI76_RS23240 0.29 -1.6 4,848,002 - BWI76_RS23240 0.34 -1.4 4,848,397 + BWI76_RS23240 0.46 -2.1 4,848,798 + BWI76_RS23240 0.58 -2.4 4,848,932 + BWI76_RS23240 0.62 -1.1 4,848,940 - BWI76_RS23240 0.62 -2.2 4,849,704 + BWI76_RS23240 0.85 -2.2 4,849,712 - BWI76_RS23240 0.85 -2.5 4,850,375 - BWI76_RS23245 0.45 -1.1 4,850,433 + BWI76_RS23245 0.63 -0.9 4,850,679 - BWI76_RS23250 0.35 -2.2 4,850,784 - BWI76_RS23250 0.61 -0.7 4,850,953 - -0.3 4,851,173 + BWI76_RS23255 0.41 -0.3
Or see this region's nucleotide sequence