Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22865 and BWI76_RS22870 are separated by 111 nucleotides BWI76_RS22870 and BWI76_RS22875 are separated by 536 nucleotides
BWI76_RS22865: BWI76_RS22865 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 4,769,917 to 4,770,762
_RS22865
BWI76_RS22870: BWI76_RS22870 - LOG family protein, at 4,770,874 to 4,772,241
_RS22870
BWI76_RS22875: BWI76_RS22875 - serine transporter, at 4,772,778 to 4,774,067
_RS22875
Position (kb)
4770
4771
4772
4773 Strain fitness (log2 ratio)
-1
0
1 at 4769.880 kb on - strand at 4769.948 kb on - strand at 4770.001 kb on - strand at 4770.003 kb on + strand, within BWI76_RS22865 at 4770.011 kb on - strand, within BWI76_RS22865 at 4770.061 kb on - strand, within BWI76_RS22865 at 4770.091 kb on + strand, within BWI76_RS22865 at 4770.099 kb on + strand, within BWI76_RS22865 at 4770.399 kb on + strand, within BWI76_RS22865 at 4770.415 kb on - strand, within BWI76_RS22865 at 4770.442 kb on + strand, within BWI76_RS22865 at 4770.830 kb on + strand at 4770.892 kb on - strand at 4771.221 kb on - strand, within BWI76_RS22870 at 4771.287 kb on + strand, within BWI76_RS22870 at 4771.295 kb on + strand, within BWI76_RS22870 at 4771.635 kb on - strand, within BWI76_RS22870 at 4771.784 kb on + strand, within BWI76_RS22870 at 4771.916 kb on - strand, within BWI76_RS22870 at 4771.989 kb on + strand, within BWI76_RS22870 at 4772.059 kb on - strand, within BWI76_RS22870 at 4772.103 kb on - strand, within BWI76_RS22870 at 4772.186 kb on + strand at 4772.217 kb on - strand at 4772.286 kb on - strand at 4772.527 kb on - strand at 4772.785 kb on - strand at 4772.889 kb on + strand at 4772.971 kb on + strand, within BWI76_RS22875 at 4773.154 kb on + strand, within BWI76_RS22875
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 4,769,880 - -0.7 4,769,948 - +0.9 4,770,001 - +0.5 4,770,003 + BWI76_RS22865 0.10 -0.8 4,770,011 - BWI76_RS22865 0.11 -0.3 4,770,061 - BWI76_RS22865 0.17 +0.8 4,770,091 + BWI76_RS22865 0.21 -0.3 4,770,099 + BWI76_RS22865 0.22 -0.0 4,770,399 + BWI76_RS22865 0.57 +1.0 4,770,415 - BWI76_RS22865 0.59 -0.1 4,770,442 + BWI76_RS22865 0.62 -0.0 4,770,830 + -0.6 4,770,892 - +0.1 4,771,221 - BWI76_RS22870 0.25 +1.1 4,771,287 + BWI76_RS22870 0.30 +0.2 4,771,295 + BWI76_RS22870 0.31 -1.1 4,771,635 - BWI76_RS22870 0.56 +0.5 4,771,784 + BWI76_RS22870 0.67 +1.0 4,771,916 - BWI76_RS22870 0.76 +1.1 4,771,989 + BWI76_RS22870 0.82 +1.7 4,772,059 - BWI76_RS22870 0.87 +0.5 4,772,103 - BWI76_RS22870 0.90 +0.1 4,772,186 + -0.0 4,772,217 - -0.5 4,772,286 - +0.0 4,772,527 - +0.6 4,772,785 - +1.2 4,772,889 + -0.0 4,772,971 + BWI76_RS22875 0.15 +0.1 4,773,154 + BWI76_RS22875 0.29 -0.0
Or see this region's nucleotide sequence