Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20015 and BWI76_RS20020 are separated by 137 nucleotides BWI76_RS20020 and BWI76_RS20025 overlap by 9 nucleotides BWI76_RS20025 and BWI76_RS20030 overlap by 14 nucleotides BWI76_RS20030 and BWI76_RS20035 are separated by 134 nucleotides
BWI76_RS20015: BWI76_RS20015 - hypothetical protein, at 4,193,769 to 4,194,785
_RS20015
BWI76_RS20020: BWI76_RS20020 - nucleoside triphosphatase NudI, at 4,194,923 to 4,195,348
_RS20020
BWI76_RS20025: BWI76_RS20025 - HAMP domain-containing histidine kinase, at 4,195,340 to 4,196,755
_RS20025
BWI76_RS20030: BWI76_RS20030 - DNA-binding response regulator, at 4,196,742 to 4,197,416
_RS20030
BWI76_RS20035: BWI76_RS20035 - hypothetical protein, at 4,197,551 to 4,198,531
_RS20035
Position (kb) 4195
4196
4197 Strain fitness (log2 ratio) -1
0
1
2 at 4194.512 kb on + strand, within BWI76_RS20015 at 4194.824 kb on - strand at 4195.091 kb on + strand, within BWI76_RS20020 at 4195.283 kb on + strand, within BWI76_RS20020 at 4195.314 kb on - strand at 4195.956 kb on + strand, within BWI76_RS20025 at 4196.001 kb on + strand, within BWI76_RS20025 at 4196.305 kb on - strand, within BWI76_RS20025 at 4196.528 kb on + strand, within BWI76_RS20025 at 4196.762 kb on + strand at 4196.811 kb on + strand, within BWI76_RS20030 at 4196.846 kb on + strand, within BWI76_RS20030 at 4197.056 kb on - strand, within BWI76_RS20030 at 4197.156 kb on - strand, within BWI76_RS20030 at 4197.233 kb on + strand, within BWI76_RS20030 at 4197.527 kb on + strand at 4197.603 kb on - strand at 4197.610 kb on + strand at 4197.613 kb on - strand at 4197.705 kb on + strand, within BWI76_RS20035
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 4,194,512 + BWI76_RS20015 0.73 -0.3 4,194,824 - -0.3 4,195,091 + BWI76_RS20020 0.39 -0.1 4,195,283 + BWI76_RS20020 0.85 -0.2 4,195,314 - -1.1 4,195,956 + BWI76_RS20025 0.44 -0.4 4,196,001 + BWI76_RS20025 0.47 -0.7 4,196,305 - BWI76_RS20025 0.68 -1.2 4,196,528 + BWI76_RS20025 0.84 -0.7 4,196,762 + -0.8 4,196,811 + BWI76_RS20030 0.10 +0.2 4,196,846 + BWI76_RS20030 0.15 +0.8 4,197,056 - BWI76_RS20030 0.47 -0.5 4,197,156 - BWI76_RS20030 0.61 +0.4 4,197,233 + BWI76_RS20030 0.73 -0.8 4,197,527 + -0.7 4,197,603 - -0.2 4,197,610 + -0.9 4,197,613 - +2.7 4,197,705 + BWI76_RS20035 0.16 +1.0
Or see this region's nucleotide sequence