Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19765

Experiment: LB in EcoFAB_10mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19760 and BWI76_RS19765 are separated by 41 nucleotidesBWI76_RS19765 and BWI76_RS19770 are separated by 426 nucleotides BWI76_RS19760: BWI76_RS19760 - GTP-binding protein, at 4,131,142 to 4,132,119 _RS19760 BWI76_RS19765: BWI76_RS19765 - membrane protein, at 4,132,161 to 4,132,868 _RS19765 BWI76_RS19770: BWI76_RS19770 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 4,133,295 to 4,133,864 _RS19770 Position (kb) 4132 4133Strain fitness (log2 ratio) -1 0 1 2 3at 4131.499 kb on - strand, within BWI76_RS19760at 4131.616 kb on + strand, within BWI76_RS19760at 4131.690 kb on + strand, within BWI76_RS19760at 4131.852 kb on - strand, within BWI76_RS19760at 4132.158 kb on - strandat 4132.295 kb on + strand, within BWI76_RS19765at 4132.314 kb on + strand, within BWI76_RS19765at 4132.374 kb on + strand, within BWI76_RS19765at 4132.382 kb on - strand, within BWI76_RS19765at 4132.446 kb on + strand, within BWI76_RS19765at 4132.537 kb on - strand, within BWI76_RS19765at 4132.585 kb on + strand, within BWI76_RS19765at 4132.729 kb on - strand, within BWI76_RS19765at 4132.775 kb on - strand, within BWI76_RS19765at 4132.792 kb on - strand, within BWI76_RS19765at 4132.810 kb on + strandat 4132.900 kb on + strandat 4133.020 kb on - strandat 4133.023 kb on + strandat 4133.124 kb on - strandat 4133.124 kb on - strandat 4133.124 kb on - strandat 4133.281 kb on + strandat 4133.289 kb on - strandat 4133.289 kb on - strandat 4133.358 kb on + strand, within BWI76_RS19770at 4133.366 kb on - strand, within BWI76_RS19770at 4133.847 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL
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4,131,499 - BWI76_RS19760 0.37 +0.2
4,131,616 + BWI76_RS19760 0.48 +3.4
4,131,690 + BWI76_RS19760 0.56 -0.2
4,131,852 - BWI76_RS19760 0.73 -0.2
4,132,158 - +0.2
4,132,295 + BWI76_RS19765 0.19 -0.0
4,132,314 + BWI76_RS19765 0.22 -0.0
4,132,374 + BWI76_RS19765 0.30 -0.3
4,132,382 - BWI76_RS19765 0.31 -0.3
4,132,446 + BWI76_RS19765 0.40 -0.2
4,132,537 - BWI76_RS19765 0.53 -0.0
4,132,585 + BWI76_RS19765 0.60 +0.1
4,132,729 - BWI76_RS19765 0.80 +0.5
4,132,775 - BWI76_RS19765 0.87 -0.3
4,132,792 - BWI76_RS19765 0.89 -0.0
4,132,810 + +3.7
4,132,900 + +0.4
4,133,020 - +0.3
4,133,023 + -0.9
4,133,124 - -0.1
4,133,124 - -0.6
4,133,124 - -0.5
4,133,281 + -0.6
4,133,289 - -0.5
4,133,289 - -0.9
4,133,358 + BWI76_RS19770 0.11 -0.4
4,133,366 - BWI76_RS19770 0.12 -0.4
4,133,847 + +1.6

Or see this region's nucleotide sequence