Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18850

Experiment: LB in EcoFAB_10mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18840 and BWI76_RS18845 overlap by 14 nucleotidesBWI76_RS18845 and BWI76_RS18850 are separated by 195 nucleotidesBWI76_RS18850 and BWI76_RS18855 are separated by 30 nucleotides BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378 _RS18840 BWI76_RS18845: BWI76_RS18845 - nitrogen fixation protein NifX, at 3,909,365 to 3,909,835 _RS18845 BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855 _RS18850 BWI76_RS18855: BWI76_RS18855 - cysteine desulfurase NifS, at 3,910,886 to 3,912,088 _RS18855 Position (kb) 3910 3911Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3909.394 kb on + strandat 3909.471 kb on - strandat 3909.538 kb on - strand, within BWI76_RS18845at 3909.610 kb on + strand, within BWI76_RS18845at 3909.735 kb on + strand, within BWI76_RS18845at 3910.023 kb on - strandat 3910.273 kb on + strand, within BWI76_RS18850at 3910.355 kb on - strand, within BWI76_RS18850at 3910.481 kb on + strand, within BWI76_RS18850at 3910.481 kb on + strand, within BWI76_RS18850at 3910.481 kb on + strand, within BWI76_RS18850at 3910.489 kb on - strand, within BWI76_RS18850at 3910.709 kb on + strand, within BWI76_RS18850at 3910.712 kb on - strand, within BWI76_RS18850at 3910.782 kb on + strandat 3911.309 kb on + strand, within BWI76_RS18855at 3911.517 kb on + strand, within BWI76_RS18855at 3911.567 kb on + strand, within BWI76_RS18855at 3911.838 kb on + strand, within BWI76_RS18855

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL
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3,909,394 + +1.0
3,909,471 - +2.8
3,909,538 - BWI76_RS18845 0.37 +0.4
3,909,610 + BWI76_RS18845 0.52 -0.2
3,909,735 + BWI76_RS18845 0.79 -0.2
3,910,023 - -0.6
3,910,273 + BWI76_RS18850 0.29 -0.1
3,910,355 - BWI76_RS18850 0.39 +0.4
3,910,481 + BWI76_RS18850 0.55 +0.9
3,910,481 + BWI76_RS18850 0.55 -0.1
3,910,481 + BWI76_RS18850 0.55 +0.4
3,910,489 - BWI76_RS18850 0.56 -0.2
3,910,709 + BWI76_RS18850 0.82 +2.9
3,910,712 - BWI76_RS18850 0.83 -0.9
3,910,782 + +0.4
3,911,309 + BWI76_RS18855 0.35 +1.1
3,911,517 + BWI76_RS18855 0.52 -0.6
3,911,567 + BWI76_RS18855 0.57 -2.2
3,911,838 + BWI76_RS18855 0.79 +1.1

Or see this region's nucleotide sequence