Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16495 and BWI76_RS16500 are separated by 121 nucleotides BWI76_RS16500 and BWI76_RS16505 are separated by 56 nucleotides BWI76_RS16505 and BWI76_RS16510 are separated by 551 nucleotides
BWI76_RS16495: BWI76_RS16495 - endopeptidase, at 3,419,170 to 3,420,024
_RS16495
BWI76_RS16500: BWI76_RS16500 - superoxide dismutase [Fe], at 3,420,146 to 3,420,727
_RS16500
BWI76_RS16505: BWI76_RS16505 - MFS transporter, at 3,420,784 to 3,421,950
_RS16505
BWI76_RS16510: BWI76_RS16510 - transcriptional repressor PurR, at 3,422,502 to 3,423,527
_RS16510
Position (kb)
3420
3421
3422 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3420.343 kb on - strand, within BWI76_RS16500 at 3420.666 kb on + strand, within BWI76_RS16500 at 3421.197 kb on + strand, within BWI76_RS16505 at 3421.322 kb on + strand, within BWI76_RS16505 at 3421.330 kb on - strand, within BWI76_RS16505 at 3421.330 kb on - strand, within BWI76_RS16505 at 3421.570 kb on - strand, within BWI76_RS16505 at 3421.632 kb on + strand, within BWI76_RS16505 at 3421.640 kb on - strand, within BWI76_RS16505 at 3422.104 kb on - strand at 3422.139 kb on - strand at 3422.385 kb on + strand at 3422.404 kb on + strand at 3422.509 kb on + strand at 3422.765 kb on + strand, within BWI76_RS16510 at 3422.766 kb on + strand, within BWI76_RS16510 at 3422.766 kb on + strand, within BWI76_RS16510
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 3,420,343 - BWI76_RS16500 0.34 -1.0 3,420,666 + BWI76_RS16500 0.89 -0.7 3,421,197 + BWI76_RS16505 0.35 -0.9 3,421,322 + BWI76_RS16505 0.46 +0.1 3,421,330 - BWI76_RS16505 0.47 -0.1 3,421,330 - BWI76_RS16505 0.47 +1.1 3,421,570 - BWI76_RS16505 0.67 +4.2 3,421,632 + BWI76_RS16505 0.73 -1.4 3,421,640 - BWI76_RS16505 0.73 -0.5 3,422,104 - -1.1 3,422,139 - -0.0 3,422,385 + -0.5 3,422,404 + -0.0 3,422,509 + +0.8 3,422,765 + BWI76_RS16510 0.26 -0.1 3,422,766 + BWI76_RS16510 0.26 -0.5 3,422,766 + BWI76_RS16510 0.26 +0.3
Or see this region's nucleotide sequence