Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotides BWI76_RS16035 and BWI76_RS16040 overlap by 1 nucleotides BWI76_RS16040 and BWI76_RS16045 are separated by 129 nucleotides
BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931
_RS16030
BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757
_RS16035
BWI76_RS16040: BWI76_RS16040 - biotin-independent malonate decarboxylase subunit gamma, at 3,325,757 to 3,326,557
_RS16040
BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646
_RS16045
Position (kb)
3325
3326
3327 Strain fitness (log2 ratio)
-1
0
1
2 at 3324.776 kb on - strand, within BWI76_RS16030 at 3324.906 kb on + strand at 3324.991 kb on - strand at 3325.238 kb on + strand, within BWI76_RS16035 at 3325.287 kb on - strand, within BWI76_RS16035 at 3325.339 kb on + strand, within BWI76_RS16035 at 3326.371 kb on + strand, within BWI76_RS16040 at 3326.379 kb on - strand, within BWI76_RS16040 at 3326.517 kb on + strand at 3326.525 kb on - strand at 3326.580 kb on - strand at 3326.582 kb on + strand at 3326.841 kb on - strand, within BWI76_RS16045 at 3327.330 kb on + strand, within BWI76_RS16045 at 3327.471 kb on + strand, within BWI76_RS16045
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 3,324,776 - BWI76_RS16030 0.48 +0.0 3,324,906 + -0.1 3,324,991 - +0.7 3,325,238 + BWI76_RS16035 0.38 -0.0 3,325,287 - BWI76_RS16035 0.44 -0.2 3,325,339 + BWI76_RS16035 0.50 +0.0 3,326,371 + BWI76_RS16040 0.77 +1.8 3,326,379 - BWI76_RS16040 0.78 +0.0 3,326,517 + -0.6 3,326,525 - +0.5 3,326,580 - -0.8 3,326,582 + -0.4 3,326,841 - BWI76_RS16045 0.16 +0.1 3,327,330 + BWI76_RS16045 0.67 -0.7 3,327,471 + BWI76_RS16045 0.82 -1.3
Or see this region's nucleotide sequence