Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16040

Experiment: LB in EcoFAB_10mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotidesBWI76_RS16035 and BWI76_RS16040 overlap by 1 nucleotidesBWI76_RS16040 and BWI76_RS16045 are separated by 129 nucleotides BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931 _RS16030 BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757 _RS16035 BWI76_RS16040: BWI76_RS16040 - biotin-independent malonate decarboxylase subunit gamma, at 3,325,757 to 3,326,557 _RS16040 BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646 _RS16045 Position (kb) 3325 3326 3327Strain fitness (log2 ratio) -1 0 1 2at 3324.776 kb on - strand, within BWI76_RS16030at 3324.906 kb on + strandat 3324.991 kb on - strandat 3325.238 kb on + strand, within BWI76_RS16035at 3325.287 kb on - strand, within BWI76_RS16035at 3325.339 kb on + strand, within BWI76_RS16035at 3326.371 kb on + strand, within BWI76_RS16040at 3326.379 kb on - strand, within BWI76_RS16040at 3326.517 kb on + strandat 3326.525 kb on - strandat 3326.580 kb on - strandat 3326.582 kb on + strandat 3326.841 kb on - strand, within BWI76_RS16045at 3327.330 kb on + strand, within BWI76_RS16045at 3327.471 kb on + strand, within BWI76_RS16045

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL
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3,324,776 - BWI76_RS16030 0.48 +0.0
3,324,906 + -0.1
3,324,991 - +0.7
3,325,238 + BWI76_RS16035 0.38 -0.0
3,325,287 - BWI76_RS16035 0.44 -0.2
3,325,339 + BWI76_RS16035 0.50 +0.0
3,326,371 + BWI76_RS16040 0.77 +1.8
3,326,379 - BWI76_RS16040 0.78 +0.0
3,326,517 + -0.6
3,326,525 - +0.5
3,326,580 - -0.8
3,326,582 + -0.4
3,326,841 - BWI76_RS16045 0.16 +0.1
3,327,330 + BWI76_RS16045 0.67 -0.7
3,327,471 + BWI76_RS16045 0.82 -1.3

Or see this region's nucleotide sequence