Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14025 and BWI76_RS14030 are separated by 24 nucleotides BWI76_RS14030 and BWI76_RS14035 overlap by 4 nucleotides BWI76_RS14035 and BWI76_RS14040 overlap by 4 nucleotides
BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475
_RS14025
BWI76_RS14030: BWI76_RS14030 - ABC transporter permease, at 2,932,500 to 2,933,240
_RS14030
BWI76_RS14035: BWI76_RS14035 - ABC transporter ATPase, at 2,933,237 to 2,933,965
_RS14035
BWI76_RS14040: BWI76_RS14040 - thiaminase, at 2,933,962 to 2,934,657
_RS14040
Position (kb)
2933
2934 Strain fitness (log2 ratio)
-1
0
1
2 at 2932.483 kb on - strand at 2932.527 kb on + strand at 2932.563 kb on + strand at 2932.789 kb on - strand, within BWI76_RS14030 at 2932.819 kb on + strand, within BWI76_RS14030 at 2933.013 kb on - strand, within BWI76_RS14030 at 2933.034 kb on - strand, within BWI76_RS14030 at 2933.228 kb on + strand at 2933.236 kb on - strand at 2933.485 kb on - strand, within BWI76_RS14035 at 2933.485 kb on - strand, within BWI76_RS14035 at 2933.616 kb on - strand, within BWI76_RS14035 at 2933.701 kb on + strand, within BWI76_RS14035 at 2933.701 kb on + strand, within BWI76_RS14035 at 2933.937 kb on - strand at 2933.994 kb on - strand at 2934.013 kb on - strand at 2934.039 kb on - strand, within BWI76_RS14040 at 2934.039 kb on - strand, within BWI76_RS14040 at 2934.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 2,932,483 - -0.8 2,932,527 + +0.4 2,932,563 + +0.7 2,932,789 - BWI76_RS14030 0.39 +2.2 2,932,819 + BWI76_RS14030 0.43 -0.7 2,933,013 - BWI76_RS14030 0.69 -1.0 2,933,034 - BWI76_RS14030 0.72 +0.1 2,933,228 + -0.1 2,933,236 - -1.4 2,933,485 - BWI76_RS14035 0.34 -0.2 2,933,485 - BWI76_RS14035 0.34 -1.0 2,933,616 - BWI76_RS14035 0.52 -0.8 2,933,701 + BWI76_RS14035 0.64 -0.9 2,933,701 + BWI76_RS14035 0.64 -1.2 2,933,937 - -0.1 2,933,994 - -0.2 2,934,013 - +2.1 2,934,039 - BWI76_RS14040 0.11 -1.0 2,934,039 - BWI76_RS14040 0.11 -0.4 2,934,870 - +0.0
Or see this region's nucleotide sequence