Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13215 and BWI76_RS13220 are separated by 243 nucleotides BWI76_RS13220 and BWI76_RS13225 are separated by 86 nucleotides BWI76_RS13225 and BWI76_RS13230 are separated by 132 nucleotides
BWI76_RS13215: BWI76_RS13215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,758,236 to 2,759,234
_RS13215
BWI76_RS13220: BWI76_RS13220 - N-acetyltransferase, at 2,759,478 to 2,759,972
_RS13220
BWI76_RS13225: BWI76_RS13225 - hypothetical protein, at 2,760,059 to 2,760,985
_RS13225
BWI76_RS13230: BWI76_RS13230 - GGDEF domain-containing protein, at 2,761,118 to 2,762,089
_RS13230
Position (kb)
2760
2761 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2759.150 kb on + strand at 2759.150 kb on + strand at 2759.153 kb on + strand at 2759.158 kb on - strand at 2759.204 kb on + strand at 2759.454 kb on + strand at 2759.524 kb on - strand at 2759.681 kb on - strand, within BWI76_RS13220 at 2759.835 kb on + strand, within BWI76_RS13220 at 2760.021 kb on - strand at 2760.034 kb on - strand at 2760.103 kb on - strand at 2760.287 kb on + strand, within BWI76_RS13225 at 2760.622 kb on + strand, within BWI76_RS13225 at 2760.635 kb on - strand, within BWI76_RS13225 at 2760.796 kb on + strand, within BWI76_RS13225 at 2760.927 kb on + strand at 2761.278 kb on + strand, within BWI76_RS13230 at 2761.776 kb on - strand, within BWI76_RS13230
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 2,759,150 + -0.9 2,759,150 + -0.0 2,759,153 + -0.1 2,759,158 - -0.7 2,759,204 + +2.4 2,759,454 + -1.8 2,759,524 - +0.3 2,759,681 - BWI76_RS13220 0.41 +1.2 2,759,835 + BWI76_RS13220 0.72 +0.3 2,760,021 - -0.5 2,760,034 - -0.3 2,760,103 - -0.3 2,760,287 + BWI76_RS13225 0.25 -0.6 2,760,622 + BWI76_RS13225 0.61 +0.3 2,760,635 - BWI76_RS13225 0.62 +3.1 2,760,796 + BWI76_RS13225 0.80 -0.2 2,760,927 + +1.2 2,761,278 + BWI76_RS13230 0.16 +5.2 2,761,776 - BWI76_RS13230 0.68 -0.6
Or see this region's nucleotide sequence