Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04160 and BWI76_RS04165 are separated by 9 nucleotides BWI76_RS04165 and BWI76_RS04170 are separated by 21 nucleotides
BWI76_RS04160: BWI76_RS04160 - phosphoserine phosphatase, at 855,672 to 856,643
_RS04160
BWI76_RS04165: BWI76_RS04165 - DNA repair protein RadA, at 856,653 to 858,035
_RS04165
BWI76_RS04170: BWI76_RS04170 - trifunctional nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase/transcriptional regulator NadR, at 858,057 to 859,289
_RS04170
Position (kb) 856
857
858
859 Strain fitness (log2 ratio) -2
-1
0
1
2 at 855.691 kb on + strand at 855.726 kb on + strand at 855.797 kb on + strand, within BWI76_RS04160 at 855.866 kb on - strand, within BWI76_RS04160 at 855.919 kb on + strand, within BWI76_RS04160 at 855.927 kb on - strand, within BWI76_RS04160 at 856.200 kb on - strand, within BWI76_RS04160 at 856.310 kb on + strand, within BWI76_RS04160 at 856.331 kb on - strand, within BWI76_RS04160 at 856.571 kb on + strand at 856.593 kb on + strand at 856.598 kb on + strand at 856.603 kb on - strand at 856.606 kb on - strand at 856.617 kb on + strand at 856.695 kb on + strand at 857.087 kb on - strand, within BWI76_RS04165 at 857.376 kb on - strand, within BWI76_RS04165 at 857.473 kb on + strand, within BWI76_RS04165 at 857.652 kb on + strand, within BWI76_RS04165 at 857.682 kb on + strand, within BWI76_RS04165 at 857.999 kb on + strand at 858.067 kb on - strand at 858.314 kb on + strand, within BWI76_RS04170 at 858.324 kb on - strand, within BWI76_RS04170 at 858.530 kb on - strand, within BWI76_RS04170 at 858.594 kb on - strand, within BWI76_RS04170 at 858.772 kb on - strand, within BWI76_RS04170 at 858.893 kb on + strand, within BWI76_RS04170
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 855,691 + +0.2 855,726 + +0.8 855,797 + BWI76_RS04160 0.13 +2.1 855,866 - BWI76_RS04160 0.20 +0.1 855,919 + BWI76_RS04160 0.25 +0.3 855,927 - BWI76_RS04160 0.26 -0.1 856,200 - BWI76_RS04160 0.54 +0.1 856,310 + BWI76_RS04160 0.66 +0.8 856,331 - BWI76_RS04160 0.68 -2.0 856,571 + -0.9 856,593 + +0.4 856,598 + +0.9 856,603 - -0.7 856,606 - -0.2 856,617 + -0.0 856,695 + -0.8 857,087 - BWI76_RS04165 0.31 -0.6 857,376 - BWI76_RS04165 0.52 -0.2 857,473 + BWI76_RS04165 0.59 -0.4 857,652 + BWI76_RS04165 0.72 -1.0 857,682 + BWI76_RS04165 0.74 -1.7 857,999 + -0.8 858,067 - -0.8 858,314 + BWI76_RS04170 0.21 -0.2 858,324 - BWI76_RS04170 0.22 -0.4 858,530 - BWI76_RS04170 0.38 -0.2 858,594 - BWI76_RS04170 0.44 -0.6 858,772 - BWI76_RS04170 0.58 -0.6 858,893 + BWI76_RS04170 0.68 -0.9
Or see this region's nucleotide sequence