Experiment: MoLS4 with L-Histidine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0374 and DVU0375 are separated by 84 nucleotides DVU0375 and DVU0376 are separated by 21 nucleotides DVU0376 and trxB-1 are separated by 3 nucleotides
DVU0374: DVU0374 - pyruvate ferredoxin/flavodoxin oxidoreductase family protein (TIGR), at 423,403 to 425,901
DVU0374
DVU0375: DVU0375 - Glu/Leu/Phe/Val dehydrogenase family protein (TIGR), at 425,986 to 427,215
DVU0375
DVU0376: DVU0376 - hypothetical protein (TIGR), at 427,237 to 427,503
DVU0376
DVU0377: trxB-1 - thioredoxin reductase (TIGR), at 427,507 to 428,424
trxB-1
Position (kb) 425
426
427
428 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 425.004 kb on + strand, within DVU0374 at 425.007 kb on - strand, within DVU0374 at 425.010 kb on + strand, within DVU0374 at 425.071 kb on + strand, within DVU0374 at 425.076 kb on + strand, within DVU0374 at 425.153 kb on - strand, within DVU0374 at 425.153 kb on - strand, within DVU0374 at 425.158 kb on + strand, within DVU0374 at 425.203 kb on + strand, within DVU0374 at 425.203 kb on + strand, within DVU0374 at 425.203 kb on + strand, within DVU0374 at 425.203 kb on + strand, within DVU0374 at 425.203 kb on + strand, within DVU0374 at 425.211 kb on - strand, within DVU0374 at 425.211 kb on - strand, within DVU0374 at 425.270 kb on + strand, within DVU0374 at 425.313 kb on - strand, within DVU0374 at 425.347 kb on + strand, within DVU0374 at 425.370 kb on + strand, within DVU0374 at 425.399 kb on + strand, within DVU0374 at 425.451 kb on + strand, within DVU0374 at 425.466 kb on + strand, within DVU0374 at 425.574 kb on + strand, within DVU0374 at 425.653 kb on + strand at 425.661 kb on - strand at 425.731 kb on + strand at 425.738 kb on + strand at 425.739 kb on - strand at 425.788 kb on + strand at 425.823 kb on - strand at 425.823 kb on - strand at 425.840 kb on - strand at 425.884 kb on - strand at 425.906 kb on + strand at 425.907 kb on - strand at 425.911 kb on + strand at 425.979 kb on + strand at 425.996 kb on + strand at 426.019 kb on - strand at 426.019 kb on - strand at 426.037 kb on + strand at 426.074 kb on - strand at 426.297 kb on + strand, within DVU0375 at 426.298 kb on - strand, within DVU0375 at 426.365 kb on - strand, within DVU0375 at 426.452 kb on + strand, within DVU0375 at 426.460 kb on - strand, within DVU0375 at 426.490 kb on - strand, within DVU0375 at 426.529 kb on - strand, within DVU0375 at 426.549 kb on + strand, within DVU0375 at 426.552 kb on - strand, within DVU0375 at 426.605 kb on + strand, within DVU0375 at 426.614 kb on + strand, within DVU0375 at 426.622 kb on - strand, within DVU0375 at 426.678 kb on + strand, within DVU0375 at 426.779 kb on - strand, within DVU0375 at 426.782 kb on + strand, within DVU0375 at 426.783 kb on + strand, within DVU0375 at 426.816 kb on - strand, within DVU0375 at 426.942 kb on - strand, within DVU0375 at 426.946 kb on - strand, within DVU0375 at 426.965 kb on - strand, within DVU0375 at 427.034 kb on + strand, within DVU0375 at 427.034 kb on + strand, within DVU0375 at 427.042 kb on - strand, within DVU0375 at 427.047 kb on + strand, within DVU0375 at 427.052 kb on + strand, within DVU0375 at 427.052 kb on + strand, within DVU0375 at 427.063 kb on + strand, within DVU0375 at 427.063 kb on + strand, within DVU0375 at 427.066 kb on - strand, within DVU0375 at 427.115 kb on + strand at 427.257 kb on + strand at 427.359 kb on - strand, within DVU0376 at 427.430 kb on + strand, within DVU0376 at 427.441 kb on + strand, within DVU0376 at 427.502 kb on - strand at 427.504 kb on + strand at 427.546 kb on - strand at 427.553 kb on - strand at 427.645 kb on + strand, within trxB-1 at 427.645 kb on + strand, within trxB-1 at 427.753 kb on + strand, within trxB-1 at 427.785 kb on + strand, within trxB-1 at 427.795 kb on + strand, within trxB-1 at 427.950 kb on - strand, within trxB-1 at 427.951 kb on + strand, within trxB-1 at 428.116 kb on + strand, within trxB-1 at 428.143 kb on - strand, within trxB-1 at 428.210 kb on - strand, within trxB-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Histidine as nutrient 10mM remove 425,004 + DVU0374 0.64 -0.5 425,007 - DVU0374 0.64 -2.9 425,010 + DVU0374 0.64 -1.7 425,071 + DVU0374 0.67 +0.2 425,076 + DVU0374 0.67 +0.1 425,153 - DVU0374 0.70 +0.3 425,153 - DVU0374 0.70 +1.6 425,158 + DVU0374 0.70 +0.6 425,203 + DVU0374 0.72 +0.2 425,203 + DVU0374 0.72 -0.0 425,203 + DVU0374 0.72 +0.4 425,203 + DVU0374 0.72 +0.5 425,203 + DVU0374 0.72 +0.3 425,211 - DVU0374 0.72 +0.0 425,211 - DVU0374 0.72 -1.2 425,270 + DVU0374 0.75 -1.3 425,313 - DVU0374 0.76 -0.1 425,347 + DVU0374 0.78 -0.2 425,370 + DVU0374 0.79 +0.6 425,399 + DVU0374 0.80 +0.1 425,451 + DVU0374 0.82 -0.7 425,466 + DVU0374 0.83 -0.4 425,574 + DVU0374 0.87 -2.5 425,653 + -0.4 425,661 - -2.7 425,731 + -0.1 425,738 + -0.2 425,739 - +0.6 425,788 + -0.2 425,823 - +0.4 425,823 - -0.5 425,840 - +0.5 425,884 - -0.8 425,906 + -2.2 425,907 - +0.3 425,911 + +0.2 425,979 + +1.4 425,996 + -0.0 426,019 - -3.0 426,019 - -0.1 426,037 + -1.0 426,074 - -0.1 426,297 + DVU0375 0.25 +0.1 426,298 - DVU0375 0.25 +0.5 426,365 - DVU0375 0.31 -0.0 426,452 + DVU0375 0.38 +1.0 426,460 - DVU0375 0.39 +1.2 426,490 - DVU0375 0.41 +0.1 426,529 - DVU0375 0.44 -0.8 426,549 + DVU0375 0.46 -1.9 426,552 - DVU0375 0.46 +0.3 426,605 + DVU0375 0.50 +0.5 426,614 + DVU0375 0.51 -0.1 426,622 - DVU0375 0.52 +0.0 426,678 + DVU0375 0.56 +0.6 426,779 - DVU0375 0.64 -1.3 426,782 + DVU0375 0.65 -0.4 426,783 + DVU0375 0.65 -0.5 426,816 - DVU0375 0.67 +0.7 426,942 - DVU0375 0.78 -1.1 426,946 - DVU0375 0.78 -0.5 426,965 - DVU0375 0.80 -1.0 427,034 + DVU0375 0.85 +0.3 427,034 + DVU0375 0.85 +0.3 427,042 - DVU0375 0.86 +0.6 427,047 + DVU0375 0.86 +0.1 427,052 + DVU0375 0.87 -1.2 427,052 + DVU0375 0.87 -0.4 427,063 + DVU0375 0.88 -0.2 427,063 + DVU0375 0.88 -0.2 427,066 - DVU0375 0.88 +0.2 427,115 + +0.6 427,257 + +0.2 427,359 - DVU0376 0.46 -0.3 427,430 + DVU0376 0.72 +0.6 427,441 + DVU0376 0.76 +0.7 427,502 - -0.7 427,504 + +0.7 427,546 - +0.2 427,553 - +0.8 427,645 + trxB-1 DVU0377 0.15 -0.8 427,645 + trxB-1 DVU0377 0.15 +0.6 427,753 + trxB-1 DVU0377 0.27 +0.0 427,785 + trxB-1 DVU0377 0.30 +1.8 427,795 + trxB-1 DVU0377 0.31 -0.7 427,950 - trxB-1 DVU0377 0.48 -2.7 427,951 + trxB-1 DVU0377 0.48 -1.8 428,116 + trxB-1 DVU0377 0.66 -1.4 428,143 - trxB-1 DVU0377 0.69 -2.5 428,210 - trxB-1 DVU0377 0.77 +0.5
Or see this region's nucleotide sequence