Experiment: MoLS4 with L-Histidine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cbiD and DVU2751 are separated by 109 nucleotides DVU2751 and DVU2752 are separated by 10 nucleotides DVU2752 and DVU2753 are separated by 109 nucleotides
DVU2750: cbiD - cobalamin biosynthesis protein CbiD (TIGR), at 2,858,736 to 2,859,905
cbiD
DVU2751: DVU2751 - hypothetical protein (TIGR), at 2,860,015 to 2,860,575
DVU2751
DVU2752: DVU2752 - rhodanese-like domain protein (TIGR), at 2,860,586 to 2,861,107
DVU2752
DVU2753: DVU2753 - C_GCAxxG_C_C family protein (TIGR), at 2,861,217 to 2,862,044
DVU2753
Position (kb)
2860
2861 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2859.143 kb on + strand, within cbiD at 2859.151 kb on - strand, within cbiD at 2859.167 kb on + strand, within cbiD at 2859.177 kb on + strand, within cbiD at 2859.209 kb on + strand, within cbiD at 2859.217 kb on - strand, within cbiD at 2859.329 kb on - strand, within cbiD at 2859.329 kb on - strand, within cbiD at 2859.338 kb on + strand, within cbiD at 2859.362 kb on - strand, within cbiD at 2859.379 kb on - strand, within cbiD at 2859.397 kb on + strand, within cbiD at 2859.397 kb on + strand, within cbiD at 2859.405 kb on - strand, within cbiD at 2859.410 kb on - strand, within cbiD at 2859.435 kb on + strand, within cbiD at 2859.508 kb on - strand, within cbiD at 2859.609 kb on + strand, within cbiD at 2859.691 kb on + strand, within cbiD at 2859.786 kb on + strand, within cbiD at 2859.802 kb on + strand at 2859.816 kb on + strand at 2859.902 kb on - strand at 2859.976 kb on + strand at 2860.089 kb on - strand, within DVU2751 at 2860.089 kb on - strand, within DVU2751 at 2860.131 kb on - strand, within DVU2751 at 2860.133 kb on + strand, within DVU2751 at 2860.136 kb on - strand, within DVU2751 at 2860.136 kb on - strand, within DVU2751 at 2860.136 kb on - strand, within DVU2751 at 2860.146 kb on - strand, within DVU2751 at 2860.162 kb on - strand, within DVU2751 at 2860.179 kb on - strand, within DVU2751 at 2860.195 kb on + strand, within DVU2751 at 2860.197 kb on + strand, within DVU2751 at 2860.205 kb on - strand, within DVU2751 at 2860.258 kb on + strand, within DVU2751 at 2860.258 kb on + strand, within DVU2751 at 2860.315 kb on - strand, within DVU2751 at 2860.354 kb on + strand, within DVU2751 at 2860.363 kb on + strand, within DVU2751 at 2860.368 kb on + strand, within DVU2751 at 2860.371 kb on - strand, within DVU2751 at 2860.378 kb on + strand, within DVU2751 at 2860.407 kb on + strand, within DVU2751 at 2860.552 kb on + strand at 2860.569 kb on + strand at 2860.577 kb on - strand at 2860.633 kb on + strand at 2860.704 kb on - strand, within DVU2752 at 2860.782 kb on - strand, within DVU2752 at 2860.805 kb on - strand, within DVU2752 at 2860.845 kb on - strand, within DVU2752 at 2860.900 kb on - strand, within DVU2752 at 2860.928 kb on - strand, within DVU2752 at 2860.948 kb on + strand, within DVU2752 at 2861.031 kb on + strand, within DVU2752 at 2861.109 kb on - strand at 2861.110 kb on - strand at 2861.262 kb on + strand at 2861.289 kb on - strand at 2861.308 kb on + strand, within DVU2753 at 2861.318 kb on + strand, within DVU2753 at 2861.318 kb on + strand, within DVU2753 at 2861.462 kb on + strand, within DVU2753 at 2861.571 kb on + strand, within DVU2753
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Histidine as nutrient 10mM remove 2,859,143 + cbiD DVU2750 0.35 -2.1 2,859,151 - cbiD DVU2750 0.35 -2.7 2,859,167 + cbiD DVU2750 0.37 +0.5 2,859,177 + cbiD DVU2750 0.38 -2.8 2,859,209 + cbiD DVU2750 0.40 -2.0 2,859,217 - cbiD DVU2750 0.41 -0.9 2,859,329 - cbiD DVU2750 0.51 +0.5 2,859,329 - cbiD DVU2750 0.51 -3.5 2,859,338 + cbiD DVU2750 0.51 -0.8 2,859,362 - cbiD DVU2750 0.54 -3.6 2,859,379 - cbiD DVU2750 0.55 -2.0 2,859,397 + cbiD DVU2750 0.56 -2.8 2,859,397 + cbiD DVU2750 0.56 -2.3 2,859,405 - cbiD DVU2750 0.57 -2.1 2,859,410 - cbiD DVU2750 0.58 -3.0 2,859,435 + cbiD DVU2750 0.60 -0.3 2,859,508 - cbiD DVU2750 0.66 +2.5 2,859,609 + cbiD DVU2750 0.75 -3.0 2,859,691 + cbiD DVU2750 0.82 -1.6 2,859,786 + cbiD DVU2750 0.90 -2.7 2,859,802 + -0.6 2,859,816 + -1.9 2,859,902 - -0.2 2,859,976 + -0.2 2,860,089 - DVU2751 0.13 -2.8 2,860,089 - DVU2751 0.13 +0.0 2,860,131 - DVU2751 0.21 -2.5 2,860,133 + DVU2751 0.21 +0.0 2,860,136 - DVU2751 0.22 -2.1 2,860,136 - DVU2751 0.22 -2.2 2,860,136 - DVU2751 0.22 -3.3 2,860,146 - DVU2751 0.23 -0.0 2,860,162 - DVU2751 0.26 -0.5 2,860,179 - DVU2751 0.29 -0.0 2,860,195 + DVU2751 0.32 +0.2 2,860,197 + DVU2751 0.32 -0.2 2,860,205 - DVU2751 0.34 -0.9 2,860,258 + DVU2751 0.43 +0.7 2,860,258 + DVU2751 0.43 -1.4 2,860,315 - DVU2751 0.53 -0.4 2,860,354 + DVU2751 0.60 -1.7 2,860,363 + DVU2751 0.62 -0.4 2,860,368 + DVU2751 0.63 -2.2 2,860,371 - DVU2751 0.63 +0.4 2,860,378 + DVU2751 0.65 +0.2 2,860,407 + DVU2751 0.70 -0.5 2,860,552 + -0.4 2,860,569 + +0.1 2,860,577 - -1.0 2,860,633 + -2.3 2,860,704 - DVU2752 0.23 +1.2 2,860,782 - DVU2752 0.38 -1.4 2,860,805 - DVU2752 0.42 +0.1 2,860,845 - DVU2752 0.50 -2.4 2,860,900 - DVU2752 0.60 +0.0 2,860,928 - DVU2752 0.66 -2.5 2,860,948 + DVU2752 0.69 -0.1 2,861,031 + DVU2752 0.85 -0.1 2,861,109 - -1.9 2,861,110 - -2.8 2,861,262 + -0.5 2,861,289 - -1.5 2,861,308 + DVU2753 0.11 -0.9 2,861,318 + DVU2753 0.12 +1.5 2,861,318 + DVU2753 0.12 -0.2 2,861,462 + DVU2753 0.30 -1.2 2,861,571 + DVU2753 0.43 -1.0
Or see this region's nucleotide sequence