Experiment: MoLS4 with L-Histidine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0832 and DVU0833 overlap by 59 nucleotides DVU0833 and rnhB overlap by 7 nucleotides rnhB and rplS are separated by 350 nucleotides rplS and trmD are separated by 53 nucleotides
DVU0832: DVU0832 - tetrapyrrole methylase family protein (TIGR), at 922,377 to 923,204
DVU0832
DVU0833: DVU0833 - conserved hypothetical protein TIGR00252 (TIGR), at 923,146 to 923,550
DVU0833
DVU0834: rnhB - ribonuclease HII (TIGR), at 923,544 to 924,206
rnhB
DVU0835: rplS - ribosomal protein L19 (TIGR), at 924,557 to 924,904
rplS
DVU0836: trmD - tRNA (guanine-N1)-methyltransferase (TIGR), at 924,958 to 926,235
trmD
Position (kb)
923
924
925 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 922.546 kb on + strand, within DVU0832 at 922.554 kb on - strand, within DVU0832 at 922.598 kb on - strand, within DVU0832 at 922.620 kb on + strand, within DVU0832 at 922.740 kb on + strand, within DVU0832 at 922.746 kb on - strand, within DVU0832 at 922.791 kb on + strand, within DVU0832 at 922.875 kb on + strand, within DVU0832 at 922.880 kb on - strand, within DVU0832 at 922.883 kb on - strand, within DVU0832 at 922.907 kb on + strand, within DVU0832 at 923.067 kb on + strand, within DVU0832 at 923.075 kb on - strand, within DVU0832 at 923.121 kb on - strand, within DVU0832 at 923.184 kb on - strand at 923.201 kb on - strand at 923.241 kb on + strand, within DVU0833 at 923.270 kb on + strand, within DVU0833 at 923.270 kb on + strand, within DVU0833 at 923.270 kb on + strand, within DVU0833 at 923.270 kb on + strand, within DVU0833 at 923.278 kb on - strand, within DVU0833 at 923.283 kb on - strand, within DVU0833 at 923.383 kb on + strand, within DVU0833 at 923.383 kb on + strand, within DVU0833 at 923.468 kb on + strand, within DVU0833 at 923.580 kb on + strand at 923.583 kb on - strand at 923.583 kb on - strand at 923.638 kb on + strand, within rnhB at 923.654 kb on - strand, within rnhB at 923.665 kb on + strand, within rnhB at 923.803 kb on + strand, within rnhB at 923.825 kb on + strand, within rnhB at 923.852 kb on - strand, within rnhB at 924.022 kb on + strand, within rnhB at 924.037 kb on + strand, within rnhB at 924.116 kb on + strand, within rnhB at 924.161 kb on - strand at 924.185 kb on + strand at 924.289 kb on + strand at 924.297 kb on - strand at 924.353 kb on - strand at 924.371 kb on + strand at 924.384 kb on - strand at 924.434 kb on - strand at 924.438 kb on + strand at 924.444 kb on - strand at 924.453 kb on + strand at 924.461 kb on - strand at 924.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Histidine as nutrient 10mM remove 922,546 + DVU0832 0.20 -1.3 922,554 - DVU0832 0.21 -1.5 922,598 - DVU0832 0.27 -0.7 922,620 + DVU0832 0.29 -0.0 922,740 + DVU0832 0.44 -0.4 922,746 - DVU0832 0.45 +0.6 922,791 + DVU0832 0.50 -0.2 922,875 + DVU0832 0.60 +0.6 922,880 - DVU0832 0.61 +0.0 922,883 - DVU0832 0.61 -1.6 922,907 + DVU0832 0.64 -2.6 923,067 + DVU0832 0.83 -0.2 923,075 - DVU0832 0.84 +0.1 923,121 - DVU0832 0.90 -2.5 923,184 - -0.8 923,201 - +0.5 923,241 + DVU0833 0.23 +1.0 923,270 + DVU0833 0.31 -0.0 923,270 + DVU0833 0.31 +0.2 923,270 + DVU0833 0.31 -0.5 923,270 + DVU0833 0.31 -0.6 923,278 - DVU0833 0.33 -2.6 923,283 - DVU0833 0.34 -0.0 923,383 + DVU0833 0.59 +0.2 923,383 + DVU0833 0.59 -0.0 923,468 + DVU0833 0.80 -0.7 923,580 + +0.3 923,583 - +0.7 923,583 - +0.2 923,638 + rnhB DVU0834 0.14 +1.4 923,654 - rnhB DVU0834 0.17 -3.3 923,665 + rnhB DVU0834 0.18 -1.3 923,803 + rnhB DVU0834 0.39 +0.5 923,825 + rnhB DVU0834 0.42 +0.1 923,852 - rnhB DVU0834 0.46 +0.1 924,022 + rnhB DVU0834 0.72 -0.6 924,037 + rnhB DVU0834 0.74 -1.7 924,116 + rnhB DVU0834 0.86 -2.8 924,161 - +0.2 924,185 + -1.4 924,289 + +0.0 924,297 - -0.4 924,353 - -2.7 924,371 + +1.0 924,384 - -0.9 924,434 - -3.0 924,438 + -0.4 924,444 - -0.5 924,453 + -0.2 924,461 - +0.4 924,469 - -0.7
Or see this region's nucleotide sequence