Experiment: MoLS4 with L-Histidine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0790 and ogt are separated by 74 nucleotides ogt and DVU0792 overlap by 7 nucleotides DVU0792 and tRNA-Ser are separated by 273 nucleotides tRNA-Ser and DVU0793 are separated by 149 nucleotides DVU0793 and fabI are separated by 2 nucleotides
DVU0790: DVU0790 - radical SAM protein, TIGR01212 family (TIGR), at 876,761 to 877,816
DVU0790
DVU0791: ogt - methylated-DNA--protein-cysteine methyltransferase, DNA-binding domain protein (TIGR), at 877,891 to 878,376
ogt
DVU0792: DVU0792 - conserved domain protein (TIGR), at 878,370 to 878,702
DVU0792
DVU_tRNA-Ser-2: tRNA-Ser - tRNA with anticodon GGA for Ser (VIMSS-AUTO), at 878,976 to 879,067
tRNA-Ser
DVU0793: DVU0793 - hypothetical protein (TIGR), at 879,217 to 879,312
DVU0793
DVU0794: fabI - enoyl-(acyl-carrier-protein) reductase (TIGR), at 879,315 to 880,079
fabI
Position (kb) 878
879 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 877.371 kb on - strand, within DVU0790 at 877.449 kb on - strand, within DVU0790 at 877.459 kb on + strand, within DVU0790 at 877.459 kb on - strand, within DVU0790 at 877.497 kb on + strand, within DVU0790 at 877.783 kb on + strand at 877.803 kb on + strand at 877.803 kb on + strand at 877.803 kb on + strand at 877.822 kb on - strand at 877.824 kb on + strand at 877.927 kb on + strand at 877.961 kb on - strand, within ogt at 877.963 kb on + strand, within ogt at 877.963 kb on + strand, within ogt at 878.031 kb on - strand, within ogt at 878.033 kb on + strand, within ogt at 878.038 kb on - strand, within ogt at 878.086 kb on + strand, within ogt at 878.104 kb on - strand, within ogt at 878.127 kb on - strand, within ogt at 878.238 kb on + strand, within ogt at 878.250 kb on + strand, within ogt at 878.250 kb on + strand, within ogt at 878.258 kb on - strand, within ogt at 878.261 kb on + strand, within ogt at 878.346 kb on + strand at 878.381 kb on + strand at 878.437 kb on + strand, within DVU0792 at 878.437 kb on + strand, within DVU0792 at 878.437 kb on + strand, within DVU0792 at 878.445 kb on - strand, within DVU0792 at 878.445 kb on - strand, within DVU0792 at 878.445 kb on - strand, within DVU0792 at 878.498 kb on + strand, within DVU0792 at 878.511 kb on + strand, within DVU0792 at 878.546 kb on - strand, within DVU0792 at 878.555 kb on + strand, within DVU0792 at 878.555 kb on + strand, within DVU0792 at 878.613 kb on - strand, within DVU0792 at 878.623 kb on + strand, within DVU0792 at 878.623 kb on + strand, within DVU0792 at 878.628 kb on + strand, within DVU0792 at 878.628 kb on + strand, within DVU0792 at 878.633 kb on + strand, within DVU0792 at 878.636 kb on - strand, within DVU0792 at 878.682 kb on - strand at 878.684 kb on + strand at 878.689 kb on + strand at 878.707 kb on - strand at 878.721 kb on + strand at 878.729 kb on - strand at 878.729 kb on - strand at 878.792 kb on + strand at 879.216 kb on - strand at 879.298 kb on + strand, within DVU0793 at 879.635 kb on + strand, within fabI
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Histidine as nutrient 10mM remove 877,371 - DVU0790 0.58 -0.6 877,449 - DVU0790 0.65 -2.0 877,459 + DVU0790 0.66 +1.0 877,459 - DVU0790 0.66 +0.9 877,497 + DVU0790 0.70 +0.4 877,783 + -2.3 877,803 + -0.5 877,803 + +0.2 877,803 + -2.7 877,822 - -0.3 877,824 + -0.4 877,927 + +0.4 877,961 - ogt DVU0791 0.14 -0.2 877,963 + ogt DVU0791 0.15 -1.5 877,963 + ogt DVU0791 0.15 +0.1 878,031 - ogt DVU0791 0.29 -1.7 878,033 + ogt DVU0791 0.29 +0.2 878,038 - ogt DVU0791 0.30 -0.0 878,086 + ogt DVU0791 0.40 -2.9 878,104 - ogt DVU0791 0.44 -0.1 878,127 - ogt DVU0791 0.49 -2.8 878,238 + ogt DVU0791 0.71 -0.7 878,250 + ogt DVU0791 0.74 -3.1 878,250 + ogt DVU0791 0.74 -0.2 878,258 - ogt DVU0791 0.76 -0.6 878,261 + ogt DVU0791 0.76 -0.0 878,346 + +1.0 878,381 + -0.9 878,437 + DVU0792 0.20 -0.6 878,437 + DVU0792 0.20 -0.5 878,437 + DVU0792 0.20 +0.2 878,445 - DVU0792 0.23 -0.6 878,445 - DVU0792 0.23 +1.0 878,445 - DVU0792 0.23 -0.7 878,498 + DVU0792 0.38 -1.5 878,511 + DVU0792 0.42 -0.3 878,546 - DVU0792 0.53 -0.4 878,555 + DVU0792 0.56 +0.1 878,555 + DVU0792 0.56 -0.6 878,613 - DVU0792 0.73 -0.9 878,623 + DVU0792 0.76 -0.4 878,623 + DVU0792 0.76 -1.5 878,628 + DVU0792 0.77 -1.0 878,628 + DVU0792 0.77 -2.4 878,633 + DVU0792 0.79 -3.4 878,636 - DVU0792 0.80 +0.6 878,682 - -0.0 878,684 + -2.9 878,689 + -2.9 878,707 - +0.6 878,721 + +0.4 878,729 - -0.1 878,729 - -2.3 878,792 + +0.7 879,216 - -0.4 879,298 + DVU0793 0.84 -3.2 879,635 + fabI DVU0794 0.42 -1.1
Or see this region's nucleotide sequence