Experiment: MoLS4 with L-Histidine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0257 and DVU0258 are separated by 160 nucleotides DVU0258 and DORF4170 are separated by 31 nucleotides DORF4170 and divK are separated by 100 nucleotides divK and mtrA are separated by 17 nucleotides
DVU0257: DVU0257 - acetyltransferase, GNAT family (TIGR), at 300,822 to 301,286
DVU0257
DVU0258: DVU0258 - sensory box histidine kinase/response regulator (TIGR), at 301,447 to 303,420
DVU0258
DORF4170: DORF4170 - no description, at 303,452 to 303,580
DORF4170
DVU0259: divK - DNA-binding response regulator (TIGR), at 303,681 to 304,070
divK
DVU0260: mtrA - response regulator (TIGR), at 304,088 to 304,489
mtrA
Position (kb) 301
302
303
304 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 300.459 kb on + strand at 300.513 kb on + strand at 300.544 kb on + strand at 300.552 kb on - strand at 300.566 kb on + strand at 300.574 kb on - strand at 300.655 kb on - strand at 300.655 kb on - strand at 300.718 kb on + strand at 300.836 kb on + strand at 300.959 kb on + strand, within DVU0257 at 301.113 kb on + strand, within DVU0257 at 301.316 kb on + strand at 301.316 kb on + strand at 301.361 kb on + strand at 301.377 kb on + strand at 301.499 kb on - strand at 301.508 kb on + strand at 301.690 kb on + strand, within DVU0258 at 301.757 kb on - strand, within DVU0258 at 301.757 kb on - strand, within DVU0258 at 301.775 kb on - strand, within DVU0258 at 301.826 kb on - strand, within DVU0258 at 301.840 kb on - strand, within DVU0258 at 301.840 kb on - strand, within DVU0258 at 301.853 kb on + strand, within DVU0258 at 301.983 kb on - strand, within DVU0258 at 302.060 kb on - strand, within DVU0258 at 302.142 kb on - strand, within DVU0258 at 302.157 kb on - strand, within DVU0258 at 302.170 kb on - strand, within DVU0258 at 302.279 kb on + strand, within DVU0258 at 302.279 kb on - strand, within DVU0258 at 302.291 kb on + strand, within DVU0258 at 302.377 kb on + strand, within DVU0258 at 302.377 kb on + strand, within DVU0258 at 302.377 kb on + strand, within DVU0258 at 302.385 kb on - strand, within DVU0258 at 302.385 kb on - strand, within DVU0258 at 302.577 kb on + strand, within DVU0258 at 302.585 kb on - strand, within DVU0258 at 302.624 kb on + strand, within DVU0258 at 302.751 kb on - strand, within DVU0258 at 302.768 kb on + strand, within DVU0258 at 302.928 kb on - strand, within DVU0258 at 303.159 kb on + strand, within DVU0258 at 303.199 kb on - strand, within DVU0258 at 303.423 kb on - strand at 303.457 kb on - strand at 303.475 kb on + strand, within DORF4170 at 303.563 kb on - strand, within DORF4170 at 303.577 kb on - strand at 303.613 kb on - strand at 303.618 kb on - strand at 303.654 kb on - strand at 303.912 kb on + strand, within divK at 303.933 kb on - strand, within divK at 303.968 kb on + strand, within divK at 304.023 kb on - strand, within divK at 304.080 kb on + strand at 304.230 kb on + strand, within mtrA at 304.294 kb on - strand, within mtrA at 304.309 kb on - strand, within mtrA at 304.309 kb on - strand, within mtrA at 304.320 kb on - strand, within mtrA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Histidine as nutrient 10mM remove 300,459 + -1.2 300,513 + -1.7 300,544 + -0.8 300,552 - -0.4 300,566 + -0.1 300,574 - +0.1 300,655 - -1.2 300,655 - +0.1 300,718 + -0.3 300,836 + -0.1 300,959 + DVU0257 0.29 +0.1 301,113 + DVU0257 0.63 +0.5 301,316 + -0.6 301,316 + -0.2 301,361 + +0.0 301,377 + -2.7 301,499 - -1.8 301,508 + +1.1 301,690 + DVU0258 0.12 -1.5 301,757 - DVU0258 0.16 -0.8 301,757 - DVU0258 0.16 +0.1 301,775 - DVU0258 0.17 +0.0 301,826 - DVU0258 0.19 -0.3 301,840 - DVU0258 0.20 -0.3 301,840 - DVU0258 0.20 -1.1 301,853 + DVU0258 0.21 -0.1 301,983 - DVU0258 0.27 -1.3 302,060 - DVU0258 0.31 -2.1 302,142 - DVU0258 0.35 -0.2 302,157 - DVU0258 0.36 +0.2 302,170 - DVU0258 0.37 -2.8 302,279 + DVU0258 0.42 +0.1 302,279 - DVU0258 0.42 +0.1 302,291 + DVU0258 0.43 -2.0 302,377 + DVU0258 0.47 +0.3 302,377 + DVU0258 0.47 -1.9 302,377 + DVU0258 0.47 -1.2 302,385 - DVU0258 0.48 -2.0 302,385 - DVU0258 0.48 -0.4 302,577 + DVU0258 0.57 +0.0 302,585 - DVU0258 0.58 -1.1 302,624 + DVU0258 0.60 +1.3 302,751 - DVU0258 0.66 -1.1 302,768 + DVU0258 0.67 -0.0 302,928 - DVU0258 0.75 -1.8 303,159 + DVU0258 0.87 +0.7 303,199 - DVU0258 0.89 -1.9 303,423 - +0.0 303,457 - +1.0 303,475 + DORF4170 0.18 +0.2 303,563 - DORF4170 0.86 -0.6 303,577 - -1.9 303,613 - -2.1 303,618 - +0.3 303,654 - -1.7 303,912 + divK DVU0259 0.59 -1.7 303,933 - divK DVU0259 0.65 -0.1 303,968 + divK DVU0259 0.74 -0.3 304,023 - divK DVU0259 0.88 -0.2 304,080 + -0.7 304,230 + mtrA DVU0260 0.35 +0.1 304,294 - mtrA DVU0260 0.51 -0.3 304,309 - mtrA DVU0260 0.55 +0.5 304,309 - mtrA DVU0260 0.55 -2.9 304,320 - mtrA DVU0260 0.58 -0.6
Or see this region's nucleotide sequence