Experiment: MoLS4 with L-Histidine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0153 and DVU0155 are separated by 467 nucleotides DVU0155 and pcrA overlap by 4 nucleotides
DVU0153: DVU0153 - hypothetical protein (TIGR), at 190,004 to 190,387
DVU0153
DVU0155: DVU0155 - type I phosphodiesterase/nucleotide pyrophosphatase family protein (TIGR), at 190,855 to 192,243
DVU0155
DVU0156: pcrA - ATP-dependent DNA helicase, UvrD/REP family (TIGR), at 192,240 to 194,399
pcrA
Position (kb) 190
191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 189.884 kb on - strand at 189.889 kb on + strand at 189.897 kb on + strand at 189.931 kb on + strand at 189.938 kb on + strand at 189.946 kb on - strand at 189.946 kb on - strand at 189.951 kb on - strand at 189.956 kb on - strand at 190.000 kb on - strand at 190.062 kb on + strand, within DVU0153 at 190.091 kb on + strand, within DVU0153 at 190.091 kb on - strand, within DVU0153 at 190.093 kb on + strand, within DVU0153 at 190.098 kb on + strand, within DVU0153 at 190.123 kb on + strand, within DVU0153 at 190.129 kb on - strand, within DVU0153 at 190.158 kb on - strand, within DVU0153 at 190.172 kb on - strand, within DVU0153 at 190.176 kb on + strand, within DVU0153 at 190.176 kb on + strand, within DVU0153 at 190.181 kb on + strand, within DVU0153 at 190.184 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.235 kb on - strand, within DVU0153 at 190.247 kb on + strand, within DVU0153 at 190.255 kb on - strand, within DVU0153 at 190.256 kb on + strand, within DVU0153 at 190.270 kb on - strand, within DVU0153 at 190.270 kb on - strand, within DVU0153 at 190.290 kb on + strand, within DVU0153 at 190.306 kb on - strand, within DVU0153 at 190.330 kb on + strand, within DVU0153 at 190.373 kb on + strand at 190.501 kb on + strand at 190.557 kb on + strand at 190.557 kb on + strand at 190.571 kb on - strand at 190.593 kb on + strand at 190.627 kb on - strand at 190.671 kb on - strand at 190.707 kb on + strand at 190.809 kb on + strand at 190.819 kb on - strand at 190.830 kb on - strand at 190.878 kb on - strand at 190.900 kb on - strand at 190.914 kb on - strand at 190.934 kb on + strand at 190.956 kb on - strand at 191.034 kb on + strand, within DVU0155 at 191.040 kb on - strand, within DVU0155 at 191.042 kb on - strand, within DVU0155 at 191.042 kb on - strand, within DVU0155 at 191.042 kb on - strand, within DVU0155 at 191.044 kb on + strand, within DVU0155 at 191.104 kb on + strand, within DVU0155 at 191.104 kb on + strand, within DVU0155 at 191.109 kb on + strand, within DVU0155 at 191.109 kb on + strand, within DVU0155 at 191.109 kb on + strand, within DVU0155 at 191.117 kb on - strand, within DVU0155 at 191.196 kb on + strand, within DVU0155 at 191.204 kb on - strand, within DVU0155 at 191.210 kb on - strand, within DVU0155 at 191.242 kb on + strand, within DVU0155 at 191.282 kb on - strand, within DVU0155 at 191.397 kb on + strand, within DVU0155 at 191.447 kb on + strand, within DVU0155 at 191.479 kb on - strand, within DVU0155 at 191.529 kb on + strand, within DVU0155 at 191.529 kb on + strand, within DVU0155 at 191.580 kb on - strand, within DVU0155 at 191.580 kb on - strand, within DVU0155 at 191.595 kb on + strand, within DVU0155 at 191.755 kb on + strand, within DVU0155 at 191.861 kb on + strand, within DVU0155 at 191.900 kb on - strand, within DVU0155 at 191.938 kb on + strand, within DVU0155 at 191.977 kb on - strand, within DVU0155 at 191.979 kb on + strand, within DVU0155 at 191.987 kb on - strand, within DVU0155 at 192.054 kb on + strand, within DVU0155 at 192.064 kb on - strand, within DVU0155 at 192.070 kb on + strand, within DVU0155 at 192.076 kb on - strand, within DVU0155 at 192.156 kb on - strand at 192.197 kb on + strand at 192.197 kb on + strand at 192.197 kb on + strand at 192.197 kb on + strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.283 kb on - strand at 192.311 kb on + strand at 192.369 kb on - strand at 192.402 kb on - strand at 192.474 kb on + strand, within pcrA at 192.474 kb on + strand, within pcrA at 192.474 kb on + strand, within pcrA at 192.552 kb on - strand, within pcrA at 192.575 kb on - strand, within pcrA at 192.597 kb on + strand, within pcrA at 192.603 kb on - strand, within pcrA at 192.605 kb on + strand, within pcrA at 192.613 kb on - strand, within pcrA at 192.636 kb on + strand, within pcrA at 192.643 kb on - strand, within pcrA at 192.671 kb on - strand, within pcrA at 192.671 kb on - strand, within pcrA at 192.714 kb on + strand, within pcrA at 192.714 kb on + strand, within pcrA at 192.722 kb on - strand, within pcrA at 192.771 kb on + strand, within pcrA at 192.798 kb on + strand, within pcrA at 192.806 kb on - strand, within pcrA at 192.849 kb on - strand, within pcrA at 192.931 kb on - strand, within pcrA at 192.977 kb on + strand, within pcrA at 192.985 kb on - strand, within pcrA at 193.116 kb on + strand, within pcrA at 193.157 kb on + strand, within pcrA at 193.172 kb on + strand, within pcrA at 193.172 kb on + strand, within pcrA at 193.180 kb on - strand, within pcrA at 193.188 kb on - strand, within pcrA at 193.216 kb on - strand, within pcrA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Histidine as nutrient 10mM remove 189,884 - -0.7 189,889 + -1.9 189,897 + -1.8 189,931 + -0.7 189,938 + +0.2 189,946 - -1.9 189,946 - +0.9 189,951 - -0.2 189,956 - -0.1 190,000 - -0.4 190,062 + DVU0153 0.15 +0.4 190,091 + DVU0153 0.23 +0.0 190,091 - DVU0153 0.23 -1.3 190,093 + DVU0153 0.23 +0.1 190,098 + DVU0153 0.24 -2.2 190,123 + DVU0153 0.31 +0.1 190,129 - DVU0153 0.33 +0.0 190,158 - DVU0153 0.40 -0.5 190,172 - DVU0153 0.44 +0.1 190,176 + DVU0153 0.45 -0.0 190,176 + DVU0153 0.45 +2.2 190,181 + DVU0153 0.46 -0.7 190,184 - DVU0153 0.47 +0.7 190,189 - DVU0153 0.48 -1.3 190,189 - DVU0153 0.48 -0.6 190,189 - DVU0153 0.48 -1.1 190,235 - DVU0153 0.60 -0.5 190,247 + DVU0153 0.63 +0.3 190,255 - DVU0153 0.65 -1.0 190,256 + DVU0153 0.66 +0.0 190,270 - DVU0153 0.69 -1.2 190,270 - DVU0153 0.69 -1.1 190,290 + DVU0153 0.74 +0.9 190,306 - DVU0153 0.79 -0.2 190,330 + DVU0153 0.85 -0.1 190,373 + +0.2 190,501 + +0.5 190,557 + +1.7 190,557 + -2.2 190,571 - +0.4 190,593 + -2.0 190,627 - -1.5 190,671 - -0.1 190,707 + +0.1 190,809 + -0.7 190,819 - -1.2 190,830 - -1.4 190,878 - -0.0 190,900 - -0.1 190,914 - -1.5 190,934 + -0.2 190,956 - -2.0 191,034 + DVU0155 0.13 +0.4 191,040 - DVU0155 0.13 -2.1 191,042 - DVU0155 0.13 -0.1 191,042 - DVU0155 0.13 -0.8 191,042 - DVU0155 0.13 -0.1 191,044 + DVU0155 0.14 -0.5 191,104 + DVU0155 0.18 -0.6 191,104 + DVU0155 0.18 -2.7 191,109 + DVU0155 0.18 -0.4 191,109 + DVU0155 0.18 -0.2 191,109 + DVU0155 0.18 -0.3 191,117 - DVU0155 0.19 +0.4 191,196 + DVU0155 0.25 -0.3 191,204 - DVU0155 0.25 -1.0 191,210 - DVU0155 0.26 +1.7 191,242 + DVU0155 0.28 +0.1 191,282 - DVU0155 0.31 -0.9 191,397 + DVU0155 0.39 -1.7 191,447 + DVU0155 0.43 +0.1 191,479 - DVU0155 0.45 -2.3 191,529 + DVU0155 0.49 -1.9 191,529 + DVU0155 0.49 -0.5 191,580 - DVU0155 0.52 -2.0 191,580 - DVU0155 0.52 -0.7 191,595 + DVU0155 0.53 -0.5 191,755 + DVU0155 0.65 +0.5 191,861 + DVU0155 0.72 -2.9 191,900 - DVU0155 0.75 -1.5 191,938 + DVU0155 0.78 -1.8 191,977 - DVU0155 0.81 -2.2 191,979 + DVU0155 0.81 +0.1 191,987 - DVU0155 0.81 -0.3 192,054 + DVU0155 0.86 -0.4 192,064 - DVU0155 0.87 +0.4 192,070 + DVU0155 0.87 -1.2 192,076 - DVU0155 0.88 +0.1 192,156 - -0.5 192,197 + +0.4 192,197 + +0.0 192,197 + -0.0 192,197 + -1.2 192,205 - +0.4 192,205 - -2.3 192,205 - -1.2 192,205 - -0.8 192,205 - +0.7 192,205 - -0.2 192,205 - -2.4 192,283 - -1.1 192,311 + +0.2 192,369 - -2.2 192,402 - -1.0 192,474 + pcrA DVU0156 0.11 -2.5 192,474 + pcrA DVU0156 0.11 -0.8 192,474 + pcrA DVU0156 0.11 -0.2 192,552 - pcrA DVU0156 0.14 -0.1 192,575 - pcrA DVU0156 0.16 -0.0 192,597 + pcrA DVU0156 0.17 +0.7 192,603 - pcrA DVU0156 0.17 -2.0 192,605 + pcrA DVU0156 0.17 +1.2 192,613 - pcrA DVU0156 0.17 +0.2 192,636 + pcrA DVU0156 0.18 -0.2 192,643 - pcrA DVU0156 0.19 +1.2 192,671 - pcrA DVU0156 0.20 +0.1 192,671 - pcrA DVU0156 0.20 -3.5 192,714 + pcrA DVU0156 0.22 -0.4 192,714 + pcrA DVU0156 0.22 +0.2 192,722 - pcrA DVU0156 0.22 -1.3 192,771 + pcrA DVU0156 0.25 -0.6 192,798 + pcrA DVU0156 0.26 -1.7 192,806 - pcrA DVU0156 0.26 -0.4 192,849 - pcrA DVU0156 0.28 -0.5 192,931 - pcrA DVU0156 0.32 -0.1 192,977 + pcrA DVU0156 0.34 -0.2 192,985 - pcrA DVU0156 0.34 -2.1 193,116 + pcrA DVU0156 0.41 -2.8 193,157 + pcrA DVU0156 0.42 -0.1 193,172 + pcrA DVU0156 0.43 -0.1 193,172 + pcrA DVU0156 0.43 -2.7 193,180 - pcrA DVU0156 0.44 -2.8 193,188 - pcrA DVU0156 0.44 -0.8 193,216 - pcrA DVU0156 0.45 -0.3
Or see this region's nucleotide sequence