Experiment: exometabolites; 10% oxygen; 5 mM nitrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22415 and RR42_RS22420 are separated by 141 nucleotides RR42_RS22420 and RR42_RS22425 are separated by 312 nucleotides RR42_RS22425 and RR42_RS22430 are separated by 8 nucleotides
RR42_RS22415: RR42_RS22415 - hypothetical protein, at 364,047 to 364,883
_RS22415
RR42_RS22420: RR42_RS22420 - penicillin-binding protein, at 365,025 to 367,448
_RS22420
RR42_RS22425: RR42_RS22425 - CDP-alcohol phosphatidyltransferase, at 367,761 to 368,429
_RS22425
RR42_RS22430: RR42_RS22430 - 2OG-Fe(II) oxygenase, at 368,438 to 369,280
_RS22430
Position (kb)
365
366
367
368 Strain fitness (log2 ratio)
-1
0
1 at 364.269 kb on + strand, within RR42_RS22415 at 364.270 kb on - strand, within RR42_RS22415 at 364.587 kb on + strand, within RR42_RS22415 at 364.588 kb on - strand, within RR42_RS22415 at 364.593 kb on + strand, within RR42_RS22415 at 364.593 kb on + strand, within RR42_RS22415 at 364.593 kb on + strand, within RR42_RS22415 at 364.620 kb on - strand, within RR42_RS22415 at 364.707 kb on + strand, within RR42_RS22415 at 364.708 kb on - strand, within RR42_RS22415 at 364.708 kb on - strand, within RR42_RS22415 at 364.737 kb on + strand, within RR42_RS22415 at 364.737 kb on + strand, within RR42_RS22415 at 364.762 kb on - strand, within RR42_RS22415 at 364.779 kb on + strand, within RR42_RS22415 at 364.821 kb on + strand at 364.822 kb on - strand at 364.833 kb on + strand at 364.834 kb on - strand at 365.139 kb on + strand at 365.139 kb on + strand at 365.139 kb on + strand at 365.139 kb on + strand at 365.286 kb on - strand, within RR42_RS22420 at 365.388 kb on - strand, within RR42_RS22420 at 365.529 kb on + strand, within RR42_RS22420 at 365.529 kb on + strand, within RR42_RS22420 at 365.556 kb on + strand, within RR42_RS22420 at 365.557 kb on - strand, within RR42_RS22420 at 365.557 kb on - strand, within RR42_RS22420 at 365.557 kb on - strand, within RR42_RS22420 at 365.580 kb on + strand, within RR42_RS22420 at 365.581 kb on - strand, within RR42_RS22420 at 365.616 kb on + strand, within RR42_RS22420 at 365.616 kb on + strand, within RR42_RS22420 at 365.616 kb on + strand, within RR42_RS22420 at 365.691 kb on + strand, within RR42_RS22420 at 365.692 kb on - strand, within RR42_RS22420 at 365.692 kb on - strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.695 kb on - strand, within RR42_RS22420 at 365.695 kb on - strand, within RR42_RS22420 at 365.695 kb on - strand, within RR42_RS22420 at 365.763 kb on + strand, within RR42_RS22420 at 365.788 kb on - strand, within RR42_RS22420 at 365.788 kb on - strand, within RR42_RS22420 at 365.788 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.923 kb on - strand, within RR42_RS22420 at 365.923 kb on - strand, within RR42_RS22420 at 366.259 kb on + strand, within RR42_RS22420 at 366.259 kb on + strand, within RR42_RS22420 at 366.495 kb on + strand, within RR42_RS22420 at 366.657 kb on + strand, within RR42_RS22420 at 366.997 kb on - strand, within RR42_RS22420 at 367.138 kb on + strand, within RR42_RS22420 at 367.618 kb on + strand at 367.618 kb on + strand at 367.619 kb on - strand at 367.619 kb on - strand at 367.640 kb on + strand at 367.712 kb on + strand at 367.713 kb on - strand at 367.715 kb on + strand at 367.715 kb on + strand at 367.716 kb on - strand at 367.716 kb on - strand at 367.809 kb on - strand at 367.809 kb on - strand at 367.953 kb on + strand, within RR42_RS22425 at 367.953 kb on + strand, within RR42_RS22425 at 368.038 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction exometabolites; 10% oxygen; 5 mM nitrate remove 364,269 + RR42_RS22415 0.27 -0.6 364,270 - RR42_RS22415 0.27 -0.3 364,587 + RR42_RS22415 0.65 +0.9 364,588 - RR42_RS22415 0.65 +0.3 364,593 + RR42_RS22415 0.65 -0.5 364,593 + RR42_RS22415 0.65 +0.3 364,593 + RR42_RS22415 0.65 -1.5 364,620 - RR42_RS22415 0.68 -0.1 364,707 + RR42_RS22415 0.79 +0.8 364,708 - RR42_RS22415 0.79 -0.3 364,708 - RR42_RS22415 0.79 -0.1 364,737 + RR42_RS22415 0.82 -0.2 364,737 + RR42_RS22415 0.82 +0.8 364,762 - RR42_RS22415 0.85 +0.5 364,779 + RR42_RS22415 0.87 +1.4 364,821 + -0.6 364,822 - -0.1 364,833 + -0.7 364,834 - -1.4 365,139 + -0.7 365,139 + -0.1 365,139 + -0.2 365,139 + -0.5 365,286 - RR42_RS22420 0.11 -0.5 365,388 - RR42_RS22420 0.15 -0.3 365,529 + RR42_RS22420 0.21 -0.4 365,529 + RR42_RS22420 0.21 -0.4 365,556 + RR42_RS22420 0.22 +0.2 365,557 - RR42_RS22420 0.22 +0.1 365,557 - RR42_RS22420 0.22 -0.3 365,557 - RR42_RS22420 0.22 +0.6 365,580 + RR42_RS22420 0.23 -0.6 365,581 - RR42_RS22420 0.23 -0.1 365,616 + RR42_RS22420 0.24 -0.4 365,616 + RR42_RS22420 0.24 +0.6 365,616 + RR42_RS22420 0.24 -1.1 365,691 + RR42_RS22420 0.27 -1.3 365,692 - RR42_RS22420 0.28 -0.4 365,692 - RR42_RS22420 0.28 -1.2 365,694 + RR42_RS22420 0.28 +1.1 365,694 + RR42_RS22420 0.28 -0.6 365,694 + RR42_RS22420 0.28 -1.5 365,694 + RR42_RS22420 0.28 +0.9 365,695 - RR42_RS22420 0.28 +0.1 365,695 - RR42_RS22420 0.28 +1.1 365,695 - RR42_RS22420 0.28 -0.4 365,763 + RR42_RS22420 0.30 -0.2 365,788 - RR42_RS22420 0.31 -0.2 365,788 - RR42_RS22420 0.31 +0.4 365,788 - RR42_RS22420 0.31 -0.6 365,914 - RR42_RS22420 0.37 -0.8 365,914 - RR42_RS22420 0.37 +0.1 365,914 - RR42_RS22420 0.37 -1.7 365,914 - RR42_RS22420 0.37 -0.3 365,923 - RR42_RS22420 0.37 -0.4 365,923 - RR42_RS22420 0.37 -0.1 366,259 + RR42_RS22420 0.51 +1.5 366,259 + RR42_RS22420 0.51 +0.1 366,495 + RR42_RS22420 0.61 -0.1 366,657 + RR42_RS22420 0.67 -0.2 366,997 - RR42_RS22420 0.81 -0.1 367,138 + RR42_RS22420 0.87 -0.1 367,618 + +0.7 367,618 + -0.0 367,619 - +0.2 367,619 - +0.5 367,640 + -0.4 367,712 + -0.6 367,713 - +0.5 367,715 + -0.7 367,715 + +0.2 367,716 - +0.3 367,716 - -0.2 367,809 - -0.3 367,809 - +0.1 367,953 + RR42_RS22425 0.29 -1.4 367,953 + RR42_RS22425 0.29 -0.7 368,038 + RR42_RS22425 0.41 -0.3 368,277 + RR42_RS22425 0.77 +0.6 368,277 + RR42_RS22425 0.77 -0.0 368,278 - RR42_RS22425 0.77 +0.0 368,278 - RR42_RS22425 0.77 +0.7 368,278 - RR42_RS22425 0.77 -1.4 368,377 - +0.7
Or see this region's nucleotide sequence