Experiment: exometabolites; 10% oxygen; 5 mM nitrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03375 and RR42_RS03380 are separated by 135 nucleotides RR42_RS03380 and RR42_RS03385 are separated by 44 nucleotides
RR42_RS03375: RR42_RS03375 - glucose-6-phosphate dehydrogenase, at 750,456 to 751,946
_RS03375
RR42_RS03380: RR42_RS03380 - glucose-6-phosphate isomerase, at 752,082 to 753,722
_RS03380
RR42_RS03385: RR42_RS03385 - sugar kinase, at 753,767 to 754,696
_RS03385
Position (kb) 752
753
754 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 751.141 kb on - strand, within RR42_RS03375 at 751.327 kb on - strand, within RR42_RS03375 at 751.327 kb on - strand, within RR42_RS03375 at 751.366 kb on - strand, within RR42_RS03375 at 751.464 kb on + strand, within RR42_RS03375 at 751.464 kb on + strand, within RR42_RS03375 at 751.528 kb on + strand, within RR42_RS03375 at 751.529 kb on - strand, within RR42_RS03375 at 751.731 kb on + strand, within RR42_RS03375 at 751.867 kb on - strand at 752.030 kb on + strand at 752.375 kb on + strand, within RR42_RS03380 at 752.376 kb on - strand, within RR42_RS03380 at 753.791 kb on + strand at 753.791 kb on + strand at 753.792 kb on - strand at 753.958 kb on - strand, within RR42_RS03385 at 754.058 kb on + strand, within RR42_RS03385 at 754.277 kb on + strand, within RR42_RS03385 at 754.297 kb on + strand, within RR42_RS03385 at 754.316 kb on + strand, within RR42_RS03385 at 754.317 kb on - strand, within RR42_RS03385 at 754.317 kb on - strand, within RR42_RS03385 at 754.317 kb on - strand, within RR42_RS03385 at 754.504 kb on + strand, within RR42_RS03385 at 754.637 kb on + strand at 754.638 kb on - strand at 754.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction exometabolites; 10% oxygen; 5 mM nitrate remove 751,141 - RR42_RS03375 0.46 -2.3 751,327 - RR42_RS03375 0.58 -1.8 751,327 - RR42_RS03375 0.58 +0.3 751,366 - RR42_RS03375 0.61 +0.9 751,464 + RR42_RS03375 0.68 -2.5 751,464 + RR42_RS03375 0.68 -0.9 751,528 + RR42_RS03375 0.72 +1.9 751,529 - RR42_RS03375 0.72 +0.7 751,731 + RR42_RS03375 0.86 -0.5 751,867 - -0.7 752,030 + -0.2 752,375 + RR42_RS03380 0.18 -0.8 752,376 - RR42_RS03380 0.18 -0.6 753,791 + -0.9 753,791 + -0.1 753,792 - -0.1 753,958 - RR42_RS03385 0.21 +0.3 754,058 + RR42_RS03385 0.31 -0.3 754,277 + RR42_RS03385 0.55 -0.9 754,297 + RR42_RS03385 0.57 +2.9 754,316 + RR42_RS03385 0.59 +1.9 754,317 - RR42_RS03385 0.59 +0.9 754,317 - RR42_RS03385 0.59 +0.9 754,317 - RR42_RS03385 0.59 +0.3 754,504 + RR42_RS03385 0.79 -1.0 754,637 + +2.9 754,638 - +1.3 754,638 - -0.7
Or see this region's nucleotide sequence