Experiment: BHIS with Anhydrotetracycline 0.00078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4220 and BT4221 are separated by 20 nucleotides BT4221 and BT4222 are separated by 321 nucleotides
BT4220: BT4220 - flotillin-like protein (NCBI ptt file), at 5,556,477 to 5,558,135
BT4220
BT4221: BT4221 - hypothetical protein (NCBI ptt file), at 5,558,156 to 5,558,653
BT4221
BT4222: BT4222 - conserved hypothetical protein (NCBI ptt file), at 5,558,975 to 5,560,048
BT4222
Position (kb)
5558
5559 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5557.185 kb on - strand, within BT4220 at 5557.599 kb on + strand, within BT4220 at 5557.662 kb on + strand, within BT4220 at 5557.662 kb on + strand, within BT4220 at 5557.663 kb on - strand, within BT4220 at 5557.668 kb on - strand, within BT4220 at 5557.741 kb on + strand, within BT4220 at 5557.741 kb on + strand, within BT4220 at 5557.742 kb on - strand, within BT4220 at 5557.742 kb on - strand, within BT4220 at 5557.743 kb on + strand, within BT4220 at 5557.744 kb on - strand, within BT4220 at 5557.746 kb on + strand, within BT4220 at 5557.746 kb on + strand, within BT4220 at 5557.747 kb on - strand, within BT4220 at 5557.747 kb on - strand, within BT4220 at 5557.836 kb on - strand, within BT4220 at 5557.884 kb on + strand, within BT4220 at 5557.984 kb on - strand at 5558.045 kb on + strand at 5558.046 kb on - strand at 5558.052 kb on - strand at 5558.101 kb on - strand at 5558.103 kb on + strand at 5558.104 kb on - strand at 5558.119 kb on - strand at 5558.157 kb on + strand at 5558.157 kb on + strand at 5558.158 kb on - strand at 5558.168 kb on + strand at 5558.279 kb on + strand, within BT4221 at 5558.284 kb on + strand, within BT4221 at 5558.286 kb on + strand, within BT4221 at 5558.294 kb on - strand, within BT4221 at 5558.311 kb on - strand, within BT4221 at 5558.472 kb on + strand, within BT4221 at 5558.472 kb on + strand, within BT4221 at 5558.473 kb on - strand, within BT4221 at 5558.476 kb on + strand, within BT4221 at 5558.477 kb on - strand, within BT4221 at 5558.477 kb on - strand, within BT4221 at 5558.477 kb on - strand, within BT4221 at 5558.477 kb on - strand, within BT4221 at 5558.478 kb on + strand, within BT4221 at 5558.479 kb on - strand, within BT4221 at 5558.526 kb on - strand, within BT4221 at 5558.527 kb on - strand, within BT4221 at 5558.537 kb on - strand, within BT4221 at 5558.564 kb on + strand, within BT4221 at 5558.564 kb on + strand, within BT4221 at 5558.564 kb on + strand, within BT4221 at 5558.565 kb on - strand, within BT4221 at 5558.565 kb on - strand, within BT4221 at 5558.565 kb on - strand, within BT4221 at 5558.585 kb on + strand, within BT4221 at 5558.588 kb on + strand, within BT4221 at 5558.614 kb on + strand at 5558.644 kb on + strand at 5558.654 kb on - strand at 5558.654 kb on - strand at 5558.670 kb on + strand at 5558.670 kb on + strand at 5558.673 kb on - strand at 5558.687 kb on + strand at 5558.729 kb on - strand at 5558.747 kb on + strand at 5558.800 kb on - strand at 5558.800 kb on - strand at 5558.808 kb on - strand at 5558.813 kb on + strand at 5558.857 kb on - strand at 5558.859 kb on - strand at 5558.859 kb on - strand at 5558.859 kb on - strand at 5558.876 kb on - strand at 5558.892 kb on - strand at 5558.892 kb on - strand at 5558.892 kb on - strand at 5558.914 kb on + strand at 5558.935 kb on + strand at 5558.935 kb on + strand at 5559.023 kb on + strand at 5559.023 kb on + strand at 5559.023 kb on + strand at 5559.024 kb on - strand at 5559.024 kb on - strand at 5559.024 kb on - strand at 5559.136 kb on + strand, within BT4222 at 5559.136 kb on + strand, within BT4222 at 5559.137 kb on - strand, within BT4222 at 5559.146 kb on + strand, within BT4222 at 5559.167 kb on + strand, within BT4222 at 5559.353 kb on - strand, within BT4222 at 5559.355 kb on - strand, within BT4222 at 5559.359 kb on - strand, within BT4222 at 5559.384 kb on + strand, within BT4222 at 5559.385 kb on - strand, within BT4222 at 5559.433 kb on + strand, within BT4222 at 5559.441 kb on + strand, within BT4222 at 5559.441 kb on + strand, within BT4222 at 5559.442 kb on - strand, within BT4222 at 5559.442 kb on - strand, within BT4222 at 5559.472 kb on + strand, within BT4222 at 5559.473 kb on - strand, within BT4222 at 5559.495 kb on - strand, within BT4222 at 5559.503 kb on + strand, within BT4222 at 5559.592 kb on - strand, within BT4222 at 5559.592 kb on - strand, within BT4222 at 5559.628 kb on - strand, within BT4222
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Anhydrotetracycline 0.00078125 mM remove 5,557,185 - BT4220 0.43 -0.9 5,557,599 + BT4220 0.68 -1.9 5,557,662 + BT4220 0.71 -0.7 5,557,662 + BT4220 0.71 -0.5 5,557,663 - BT4220 0.71 -2.5 5,557,668 - BT4220 0.72 -2.1 5,557,741 + BT4220 0.76 +0.7 5,557,741 + BT4220 0.76 -2.2 5,557,742 - BT4220 0.76 -1.5 5,557,742 - BT4220 0.76 +1.1 5,557,743 + BT4220 0.76 -2.2 5,557,744 - BT4220 0.76 -0.2 5,557,746 + BT4220 0.76 -0.9 5,557,746 + BT4220 0.76 -0.8 5,557,747 - BT4220 0.77 -0.1 5,557,747 - BT4220 0.77 -0.9 5,557,836 - BT4220 0.82 -1.5 5,557,884 + BT4220 0.85 -1.5 5,557,984 - -1.9 5,558,045 + -0.5 5,558,046 - +0.1 5,558,052 - -2.5 5,558,101 - -1.5 5,558,103 + -0.9 5,558,104 - -1.0 5,558,119 - -1.1 5,558,157 + -3.1 5,558,157 + -1.5 5,558,158 - -0.9 5,558,168 + -1.4 5,558,279 + BT4221 0.25 +2.4 5,558,284 + BT4221 0.26 -3.0 5,558,286 + BT4221 0.26 -2.7 5,558,294 - BT4221 0.28 -0.9 5,558,311 - BT4221 0.31 -1.9 5,558,472 + BT4221 0.63 +0.1 5,558,472 + BT4221 0.63 -2.1 5,558,473 - BT4221 0.64 -3.1 5,558,476 + BT4221 0.64 -1.4 5,558,477 - BT4221 0.64 +0.5 5,558,477 - BT4221 0.64 -2.9 5,558,477 - BT4221 0.64 -0.9 5,558,477 - BT4221 0.64 -1.9 5,558,478 + BT4221 0.65 -0.5 5,558,479 - BT4221 0.65 -0.5 5,558,526 - BT4221 0.74 -0.3 5,558,527 - BT4221 0.74 -1.9 5,558,537 - BT4221 0.77 -2.7 5,558,564 + BT4221 0.82 +0.1 5,558,564 + BT4221 0.82 -1.3 5,558,564 + BT4221 0.82 -2.4 5,558,565 - BT4221 0.82 -3.6 5,558,565 - BT4221 0.82 -0.9 5,558,565 - BT4221 0.82 -3.2 5,558,585 + BT4221 0.86 -0.9 5,558,588 + BT4221 0.87 -3.9 5,558,614 + +2.1 5,558,644 + -0.8 5,558,654 - -2.5 5,558,654 - -3.8 5,558,670 + -2.2 5,558,670 + -0.7 5,558,673 - -0.3 5,558,687 + -1.5 5,558,729 - -1.7 5,558,747 + +0.1 5,558,800 - +0.1 5,558,800 - -1.5 5,558,808 - +0.1 5,558,813 + -0.9 5,558,857 - -1.5 5,558,859 - -0.9 5,558,859 - -0.9 5,558,859 - -0.9 5,558,876 - -1.3 5,558,892 - +0.1 5,558,892 - -2.5 5,558,892 - -1.5 5,558,914 + -1.7 5,558,935 + -0.9 5,558,935 + -1.0 5,559,023 + -1.0 5,559,023 + -0.3 5,559,023 + -1.8 5,559,024 - -0.9 5,559,024 - +1.1 5,559,024 - -0.9 5,559,136 + BT4222 0.15 -0.5 5,559,136 + BT4222 0.15 -1.5 5,559,137 - BT4222 0.15 -2.2 5,559,146 + BT4222 0.16 +0.4 5,559,167 + BT4222 0.18 -0.5 5,559,353 - BT4222 0.35 -1.3 5,559,355 - BT4222 0.35 -0.4 5,559,359 - BT4222 0.36 -1.5 5,559,384 + BT4222 0.38 -3.5 5,559,385 - BT4222 0.38 +0.1 5,559,433 + BT4222 0.43 -1.2 5,559,441 + BT4222 0.43 -0.5 5,559,441 + BT4222 0.43 -0.9 5,559,442 - BT4222 0.43 -1.2 5,559,442 - BT4222 0.43 +0.6 5,559,472 + BT4222 0.46 -1.4 5,559,473 - BT4222 0.46 +0.1 5,559,495 - BT4222 0.48 -1.2 5,559,503 + BT4222 0.49 -1.9 5,559,592 - BT4222 0.57 -2.7 5,559,592 - BT4222 0.57 +0.1 5,559,628 - BT4222 0.61 -0.9
Or see this region's nucleotide sequence