Experiment: BHIS with Anhydrotetracycline 0.00078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0654 and BT0655 are separated by 155 nucleotides BT0655 and BT0656 are separated by 112 nucleotides BT0656 and BT0657 are separated by 147 nucleotides
BT0654: BT0654 - two-component sensor histidine kinase (NCBI ptt file), at 803,832 to 805,814
BT0654
BT0655: BT0655 - superoxide dismutase [Fe] (NCBI ptt file), at 805,970 to 806,551
BT0655
BT0656: BT0656 - conserved hypothetical protein (NCBI ptt file), at 806,664 to 808,406
BT0656
BT0657: BT0657 - ATP-dependent DNA helicase (NCBI ptt file), at 808,554 to 810,920
BT0657
Position (kb)
806
807
808
809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 805.669 kb on + strand at 805.686 kb on - strand at 805.814 kb on + strand at 805.869 kb on + strand at 805.942 kb on + strand at 805.948 kb on - strand at 805.948 kb on - strand at 806.022 kb on - strand at 806.322 kb on - strand, within BT0655 at 806.360 kb on - strand, within BT0655 at 806.360 kb on - strand, within BT0655 at 806.409 kb on + strand, within BT0655 at 806.444 kb on + strand, within BT0655 at 806.445 kb on - strand, within BT0655 at 806.448 kb on + strand, within BT0655 at 806.523 kb on + strand at 806.565 kb on - strand at 806.573 kb on + strand at 806.574 kb on - strand at 806.576 kb on + strand at 806.578 kb on + strand at 806.669 kb on + strand at 806.837 kb on + strand at 806.837 kb on + strand at 806.838 kb on - strand at 806.863 kb on + strand, within BT0656 at 806.988 kb on - strand, within BT0656 at 807.082 kb on + strand, within BT0656 at 807.295 kb on + strand, within BT0656 at 807.296 kb on - strand, within BT0656 at 807.305 kb on + strand, within BT0656 at 807.597 kb on - strand, within BT0656 at 807.599 kb on - strand, within BT0656 at 807.680 kb on - strand, within BT0656 at 807.680 kb on - strand, within BT0656 at 807.810 kb on + strand, within BT0656 at 807.811 kb on - strand, within BT0656 at 807.868 kb on + strand, within BT0656 at 807.869 kb on - strand, within BT0656 at 808.429 kb on + strand at 808.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Anhydrotetracycline 0.00078125 mM remove 805,669 + -1.8 805,686 - +0.2 805,814 + +0.2 805,869 + -1.8 805,942 + -0.9 805,948 - +0.2 805,948 - -1.8 806,022 - -1.8 806,322 - BT0655 0.60 -0.6 806,360 - BT0655 0.67 -0.8 806,360 - BT0655 0.67 +0.6 806,409 + BT0655 0.75 -1.8 806,444 + BT0655 0.81 -1.8 806,445 - BT0655 0.82 +0.2 806,448 + BT0655 0.82 -3.0 806,523 + -1.4 806,565 - +0.2 806,573 + -0.8 806,574 - +0.2 806,576 + -1.7 806,578 + -1.8 806,669 + -1.8 806,837 + +0.2 806,837 + -0.2 806,838 - -0.4 806,863 + BT0656 0.11 -1.7 806,988 - BT0656 0.19 +1.8 807,082 + BT0656 0.24 -0.8 807,295 + BT0656 0.36 -0.8 807,296 - BT0656 0.36 +1.2 807,305 + BT0656 0.37 +0.2 807,597 - BT0656 0.54 -1.4 807,599 - BT0656 0.54 -0.8 807,680 - BT0656 0.58 -2.3 807,680 - BT0656 0.58 -0.8 807,810 + BT0656 0.66 -2.0 807,811 - BT0656 0.66 -3.1 807,868 + BT0656 0.69 -2.8 807,869 - BT0656 0.69 -0.8 808,429 + -1.8 808,553 + -2.4
Or see this region's nucleotide sequence