Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifD and glnB-2 are separated by 11 nucleotides glnB-2 and glnB-3 are separated by 9 nucleotides glnB-3 and nifH are separated by 282 nucleotides
DVUA0012: nifD - nitrogenase molybdenum-iron protein alpha chain (TIGR), at 16,223 to 17,851
nifD
DVUA0013: glnB-2 - nitrogen regulatory protein P-II (TIGR), at 17,863 to 18,258
glnB-2
DVUA0014: glnB-3 - nitrogen regulatory protein P-II (TIGR), at 18,268 to 18,594
glnB-3
DVUA0015: nifH - nitrogenase iron protein (TIGR), at 18,877 to 19,710
nifH
Position (kb) 17
18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 16.866 kb on - strand, within nifD at 16.920 kb on + strand, within nifD at 16.923 kb on - strand, within nifD at 16.928 kb on - strand, within nifD at 16.994 kb on - strand, within nifD at 17.040 kb on + strand, within nifD at 17.064 kb on + strand, within nifD at 17.072 kb on - strand, within nifD at 17.100 kb on + strand, within nifD at 17.131 kb on - strand, within nifD at 17.173 kb on - strand, within nifD at 17.250 kb on + strand, within nifD at 17.282 kb on + strand, within nifD at 17.288 kb on - strand, within nifD at 17.295 kb on + strand, within nifD at 17.305 kb on - strand, within nifD at 17.366 kb on + strand, within nifD at 17.405 kb on - strand, within nifD at 17.438 kb on + strand, within nifD at 17.557 kb on + strand, within nifD at 17.600 kb on + strand, within nifD at 17.655 kb on - strand, within nifD at 17.668 kb on + strand, within nifD at 17.668 kb on + strand, within nifD at 17.694 kb on + strand at 17.701 kb on - strand at 17.786 kb on + strand at 17.831 kb on - strand at 17.843 kb on - strand at 17.919 kb on - strand, within glnB-2 at 17.997 kb on + strand, within glnB-2 at 18.002 kb on + strand, within glnB-2 at 18.061 kb on - strand, within glnB-2 at 18.085 kb on - strand, within glnB-2 at 18.186 kb on + strand, within glnB-2 at 18.242 kb on - strand at 18.282 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.353 kb on + strand, within glnB-3 at 18.370 kb on + strand, within glnB-3 at 18.378 kb on - strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.431 kb on + strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.466 kb on - strand, within glnB-3 at 18.473 kb on - strand, within glnB-3 at 18.492 kb on - strand, within glnB-3 at 18.506 kb on - strand, within glnB-3 at 18.527 kb on + strand, within glnB-3 at 18.535 kb on - strand, within glnB-3 at 18.559 kb on - strand, within glnB-3 at 18.747 kb on + strand at 18.755 kb on - strand at 18.849 kb on - strand at 18.884 kb on + strand at 18.922 kb on - strand at 18.994 kb on - strand, within nifH at 19.054 kb on + strand, within nifH
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 16,866 - nifD DVUA0012 0.39 -0.6 16,920 + nifD DVUA0012 0.43 +1.9 16,923 - nifD DVUA0012 0.43 -1.0 16,928 - nifD DVUA0012 0.43 -0.5 16,994 - nifD DVUA0012 0.47 -0.9 17,040 + nifD DVUA0012 0.50 +2.9 17,064 + nifD DVUA0012 0.52 +0.6 17,072 - nifD DVUA0012 0.52 +0.9 17,100 + nifD DVUA0012 0.54 +0.9 17,131 - nifD DVUA0012 0.56 -1.1 17,173 - nifD DVUA0012 0.58 +0.7 17,250 + nifD DVUA0012 0.63 -2.0 17,282 + nifD DVUA0012 0.65 -1.3 17,288 - nifD DVUA0012 0.65 +3.4 17,295 + nifD DVUA0012 0.66 -1.1 17,305 - nifD DVUA0012 0.66 -1.4 17,366 + nifD DVUA0012 0.70 -1.1 17,405 - nifD DVUA0012 0.73 -0.9 17,438 + nifD DVUA0012 0.75 -0.8 17,557 + nifD DVUA0012 0.82 +0.1 17,600 + nifD DVUA0012 0.85 +0.1 17,655 - nifD DVUA0012 0.88 -1.4 17,668 + nifD DVUA0012 0.89 +2.4 17,668 + nifD DVUA0012 0.89 +1.2 17,694 + -1.4 17,701 - -1.0 17,786 + -1.1 17,831 - -2.4 17,843 - +1.1 17,919 - glnB-2 DVUA0013 0.14 +0.2 17,997 + glnB-2 DVUA0013 0.34 -0.6 18,002 + glnB-2 DVUA0013 0.35 -2.1 18,061 - glnB-2 DVUA0013 0.50 -1.8 18,085 - glnB-2 DVUA0013 0.56 +1.2 18,186 + glnB-2 DVUA0013 0.82 +1.1 18,242 - -1.3 18,282 + -1.2 18,296 + +2.9 18,296 + +0.9 18,296 + +1.5 18,304 - glnB-3 DVUA0014 0.11 -1.6 18,304 - glnB-3 DVUA0014 0.11 +1.1 18,304 - glnB-3 DVUA0014 0.11 +2.4 18,304 - glnB-3 DVUA0014 0.11 +0.2 18,353 + glnB-3 DVUA0014 0.26 -0.7 18,370 + glnB-3 DVUA0014 0.31 -0.4 18,378 - glnB-3 DVUA0014 0.34 +0.6 18,404 + glnB-3 DVUA0014 0.42 -2.0 18,404 + glnB-3 DVUA0014 0.42 -1.1 18,431 + glnB-3 DVUA0014 0.50 -0.2 18,439 - glnB-3 DVUA0014 0.52 +1.5 18,439 - glnB-3 DVUA0014 0.52 -1.7 18,466 - glnB-3 DVUA0014 0.61 -2.3 18,473 - glnB-3 DVUA0014 0.63 -0.2 18,492 - glnB-3 DVUA0014 0.69 -0.7 18,506 - glnB-3 DVUA0014 0.73 -1.5 18,527 + glnB-3 DVUA0014 0.79 +0.5 18,535 - glnB-3 DVUA0014 0.82 -1.1 18,559 - glnB-3 DVUA0014 0.89 -0.6 18,747 + -2.4 18,755 - -0.9 18,849 - +0.7 18,884 + -1.7 18,922 - -3.0 18,994 - nifH DVUA0015 0.14 -0.3 19,054 + nifH DVUA0015 0.21 -0.4
Or see this region's nucleotide sequence