Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flrC and flhB are separated by 47 nucleotides flhB and escT overlap by 4 nucleotides escT and invX are separated by 6 nucleotides
DVUA0100: flrC - sigma-54 dependent transcriptional regulator (TIGR), at 136,112 to 137,839
flrC
DVUA0101: flhB - type III secretion protein, hrpY/hrcU family (TIGR), at 137,887 to 138,927
flhB
DVUA0102: escT - type III secretion inner membrane protein (TIGR), at 138,924 to 139,739
escT
DVUA0103: invX - type III secretion protein, HrpO family (TIGR), at 139,746 to 140,006
invX
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 136.912 kb on + strand, within flrC at 136.958 kb on - strand, within flrC at 137.015 kb on - strand, within flrC at 137.144 kb on + strand, within flrC at 137.151 kb on + strand, within flrC at 137.161 kb on + strand, within flrC at 137.166 kb on + strand, within flrC at 137.188 kb on + strand, within flrC at 137.382 kb on + strand, within flrC at 137.397 kb on + strand, within flrC at 137.477 kb on + strand, within flrC at 137.572 kb on - strand, within flrC at 137.651 kb on - strand, within flrC at 137.653 kb on + strand, within flrC at 137.661 kb on - strand, within flrC at 137.692 kb on - strand at 137.715 kb on + strand at 137.841 kb on - strand at 137.885 kb on + strand at 137.912 kb on - strand at 137.922 kb on - strand at 138.101 kb on - strand, within flhB at 138.106 kb on - strand, within flhB at 138.148 kb on - strand, within flhB at 138.263 kb on + strand, within flhB at 138.270 kb on + strand, within flhB at 138.271 kb on - strand, within flhB at 138.315 kb on + strand, within flhB at 138.323 kb on - strand, within flhB at 138.341 kb on + strand, within flhB at 138.403 kb on - strand, within flhB at 138.408 kb on + strand, within flhB at 138.458 kb on - strand, within flhB at 138.575 kb on + strand, within flhB at 138.599 kb on - strand, within flhB at 138.622 kb on - strand, within flhB at 138.855 kb on - strand at 138.855 kb on - strand at 139.039 kb on + strand, within escT at 139.049 kb on - strand, within escT at 139.066 kb on + strand, within escT at 139.081 kb on - strand, within escT at 139.234 kb on + strand, within escT at 139.234 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.276 kb on - strand, within escT at 139.365 kb on - strand, within escT at 139.374 kb on - strand, within escT at 139.415 kb on + strand, within escT at 139.433 kb on - strand, within escT at 139.467 kb on - strand, within escT at 139.483 kb on + strand, within escT at 139.639 kb on - strand, within escT at 139.683 kb on - strand at 139.699 kb on - strand at 139.699 kb on - strand at 139.801 kb on - strand, within invX at 139.848 kb on - strand, within invX at 139.855 kb on - strand, within invX at 139.860 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.903 kb on - strand, within invX at 139.903 kb on - strand, within invX at 139.905 kb on + strand, within invX
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 136,912 + flrC DVUA0100 0.46 -0.4 136,958 - flrC DVUA0100 0.49 +2.6 137,015 - flrC DVUA0100 0.52 -1.4 137,144 + flrC DVUA0100 0.60 -0.2 137,151 + flrC DVUA0100 0.60 +0.3 137,161 + flrC DVUA0100 0.61 -0.9 137,166 + flrC DVUA0100 0.61 +1.8 137,188 + flrC DVUA0100 0.62 -0.4 137,382 + flrC DVUA0100 0.73 -0.4 137,397 + flrC DVUA0100 0.74 -1.6 137,477 + flrC DVUA0100 0.79 -0.4 137,572 - flrC DVUA0100 0.84 -0.8 137,651 - flrC DVUA0100 0.89 -0.5 137,653 + flrC DVUA0100 0.89 -2.5 137,661 - flrC DVUA0100 0.90 +0.3 137,692 - -1.0 137,715 + +1.8 137,841 - -1.3 137,885 + +1.2 137,912 - -0.4 137,922 - -0.9 138,101 - flhB DVUA0101 0.21 -1.0 138,106 - flhB DVUA0101 0.21 -1.0 138,148 - flhB DVUA0101 0.25 +1.9 138,263 + flhB DVUA0101 0.36 +0.2 138,270 + flhB DVUA0101 0.37 -0.7 138,271 - flhB DVUA0101 0.37 -0.3 138,315 + flhB DVUA0101 0.41 -1.4 138,323 - flhB DVUA0101 0.42 +0.6 138,341 + flhB DVUA0101 0.44 +0.1 138,403 - flhB DVUA0101 0.50 -0.0 138,408 + flhB DVUA0101 0.50 +3.9 138,458 - flhB DVUA0101 0.55 +0.7 138,575 + flhB DVUA0101 0.66 +0.8 138,599 - flhB DVUA0101 0.68 -1.7 138,622 - flhB DVUA0101 0.71 -0.1 138,855 - -1.9 138,855 - +1.7 139,039 + escT DVUA0102 0.14 +2.4 139,049 - escT DVUA0102 0.15 -1.4 139,066 + escT DVUA0102 0.17 -0.7 139,081 - escT DVUA0102 0.19 -0.9 139,234 + escT DVUA0102 0.38 -0.2 139,234 + escT DVUA0102 0.38 +4.9 139,258 + escT DVUA0102 0.41 -1.7 139,258 + escT DVUA0102 0.41 +0.3 139,258 + escT DVUA0102 0.41 +0.5 139,258 + escT DVUA0102 0.41 +0.2 139,258 + escT DVUA0102 0.41 -0.7 139,258 + escT DVUA0102 0.41 -0.5 139,258 + escT DVUA0102 0.41 -0.3 139,258 + escT DVUA0102 0.41 +1.8 139,266 - escT DVUA0102 0.42 -0.9 139,266 - escT DVUA0102 0.42 -1.2 139,266 - escT DVUA0102 0.42 -0.8 139,266 - escT DVUA0102 0.42 +1.5 139,266 - escT DVUA0102 0.42 +2.1 139,266 - escT DVUA0102 0.42 -1.6 139,276 - escT DVUA0102 0.43 +0.2 139,365 - escT DVUA0102 0.54 +0.4 139,374 - escT DVUA0102 0.55 -1.2 139,415 + escT DVUA0102 0.60 +2.9 139,433 - escT DVUA0102 0.62 -1.4 139,467 - escT DVUA0102 0.67 -2.3 139,483 + escT DVUA0102 0.69 -0.9 139,639 - escT DVUA0102 0.88 +0.5 139,683 - -0.8 139,699 - -0.4 139,699 - +0.3 139,801 - invX DVUA0103 0.21 -1.1 139,848 - invX DVUA0103 0.39 -2.1 139,855 - invX DVUA0103 0.42 -1.5 139,860 + invX DVUA0103 0.44 -0.7 139,895 + invX DVUA0103 0.57 +1.0 139,895 + invX DVUA0103 0.57 -0.6 139,895 + invX DVUA0103 0.57 -2.6 139,895 + invX DVUA0103 0.57 +1.5 139,895 + invX DVUA0103 0.57 -0.7 139,903 - invX DVUA0103 0.60 +1.6 139,903 - invX DVUA0103 0.60 -1.3 139,905 + invX DVUA0103 0.61 -0.4
Or see this region's nucleotide sequence