Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0278 and DVU0279 are separated by 324 nucleotides DVU0279 and wbaZ-2 are separated by 312 nucleotides
DVU0278: DVU0278 - glyoxalase family protein (TIGR), at 321,631 to 322,053
DVU0278
DVU0279: DVU0279 - sulfate permease family protein (TIGR), at 322,378 to 324,084
DVU0279
DVU0280: wbaZ-2 - glycosyl transferase, group 1 family protein (TIGR), at 324,397 to 325,536
wbaZ-2
Position (kb) 322
323
324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 321.441 kb on + strand at 321.497 kb on - strand at 321.570 kb on + strand at 321.674 kb on - strand, within DVU0278 at 321.767 kb on - strand, within DVU0278 at 321.807 kb on - strand, within DVU0278 at 321.837 kb on + strand, within DVU0278 at 321.837 kb on + strand, within DVU0278 at 321.842 kb on + strand, within DVU0278 at 321.845 kb on - strand, within DVU0278 at 321.907 kb on - strand, within DVU0278 at 321.909 kb on + strand, within DVU0278 at 321.909 kb on + strand, within DVU0278 at 321.915 kb on + strand, within DVU0278 at 321.948 kb on - strand, within DVU0278 at 321.970 kb on + strand, within DVU0278 at 322.011 kb on + strand at 322.059 kb on + strand at 322.067 kb on - strand at 322.067 kb on - strand at 322.074 kb on + strand at 322.082 kb on + strand at 322.107 kb on - strand at 322.138 kb on + strand at 322.146 kb on - strand at 322.323 kb on + strand at 322.351 kb on - strand at 322.435 kb on + strand at 322.447 kb on + strand at 322.666 kb on - strand, within DVU0279 at 322.699 kb on - strand, within DVU0279 at 322.704 kb on - strand, within DVU0279 at 322.718 kb on - strand, within DVU0279 at 322.725 kb on + strand, within DVU0279 at 322.899 kb on - strand, within DVU0279 at 322.900 kb on + strand, within DVU0279 at 323.038 kb on + strand, within DVU0279 at 323.059 kb on - strand, within DVU0279 at 323.101 kb on - strand, within DVU0279 at 323.116 kb on - strand, within DVU0279 at 323.129 kb on + strand, within DVU0279 at 323.197 kb on + strand, within DVU0279 at 323.212 kb on - strand, within DVU0279 at 323.382 kb on - strand, within DVU0279 at 323.469 kb on - strand, within DVU0279 at 323.485 kb on - strand, within DVU0279 at 323.544 kb on - strand, within DVU0279 at 323.623 kb on - strand, within DVU0279 at 323.655 kb on + strand, within DVU0279 at 323.663 kb on - strand, within DVU0279 at 323.663 kb on - strand, within DVU0279 at 323.705 kb on - strand, within DVU0279 at 323.710 kb on + strand, within DVU0279 at 323.721 kb on + strand, within DVU0279 at 323.736 kb on + strand, within DVU0279 at 323.736 kb on - strand, within DVU0279 at 323.852 kb on - strand, within DVU0279 at 323.852 kb on - strand, within DVU0279 at 323.884 kb on - strand, within DVU0279 at 323.884 kb on - strand, within DVU0279 at 323.906 kb on - strand, within DVU0279 at 323.925 kb on + strand at 323.945 kb on - strand at 324.032 kb on + strand at 324.057 kb on + strand at 324.065 kb on - strand at 324.074 kb on + strand at 324.077 kb on - strand at 324.079 kb on + strand at 324.082 kb on - strand at 324.169 kb on - strand at 324.183 kb on + strand at 324.183 kb on + strand at 324.183 kb on + strand at 324.191 kb on - strand at 324.191 kb on - strand at 324.325 kb on - strand at 324.561 kb on + strand, within wbaZ-2 at 324.640 kb on + strand, within wbaZ-2 at 324.712 kb on + strand, within wbaZ-2 at 324.712 kb on + strand, within wbaZ-2 at 324.824 kb on - strand, within wbaZ-2 at 324.868 kb on + strand, within wbaZ-2 at 324.982 kb on + strand, within wbaZ-2
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 321,441 + -0.8 321,497 - +0.3 321,570 + -1.7 321,674 - DVU0278 0.10 -0.7 321,767 - DVU0278 0.32 -0.8 321,807 - DVU0278 0.42 -1.1 321,837 + DVU0278 0.49 +1.0 321,837 + DVU0278 0.49 -1.9 321,842 + DVU0278 0.50 -1.8 321,845 - DVU0278 0.51 -0.9 321,907 - DVU0278 0.65 -1.7 321,909 + DVU0278 0.66 -0.8 321,909 + DVU0278 0.66 -0.7 321,915 + DVU0278 0.67 +3.4 321,948 - DVU0278 0.75 -1.1 321,970 + DVU0278 0.80 +0.8 322,011 + -0.9 322,059 + +1.8 322,067 - +0.6 322,067 - -1.3 322,074 + +0.5 322,082 + -0.3 322,107 - +1.8 322,138 + +2.1 322,146 - -1.6 322,323 + -1.3 322,351 - -1.7 322,435 + -1.8 322,447 + -0.5 322,666 - DVU0279 0.17 +0.1 322,699 - DVU0279 0.19 -0.8 322,704 - DVU0279 0.19 +0.1 322,718 - DVU0279 0.20 +0.6 322,725 + DVU0279 0.20 -0.4 322,899 - DVU0279 0.31 +0.1 322,900 + DVU0279 0.31 -0.3 323,038 + DVU0279 0.39 -0.3 323,059 - DVU0279 0.40 +1.4 323,101 - DVU0279 0.42 -1.8 323,116 - DVU0279 0.43 -0.4 323,129 + DVU0279 0.44 +0.6 323,197 + DVU0279 0.48 +2.9 323,212 - DVU0279 0.49 -0.7 323,382 - DVU0279 0.59 +2.9 323,469 - DVU0279 0.64 -0.3 323,485 - DVU0279 0.65 -0.1 323,544 - DVU0279 0.68 -0.4 323,623 - DVU0279 0.73 -0.1 323,655 + DVU0279 0.75 +0.4 323,663 - DVU0279 0.75 +2.4 323,663 - DVU0279 0.75 -0.2 323,705 - DVU0279 0.78 -0.4 323,710 + DVU0279 0.78 -0.4 323,721 + DVU0279 0.79 -1.4 323,736 + DVU0279 0.80 -1.0 323,736 - DVU0279 0.80 -0.5 323,852 - DVU0279 0.86 +1.0 323,852 - DVU0279 0.86 +0.9 323,884 - DVU0279 0.88 -0.4 323,884 - DVU0279 0.88 +1.9 323,906 - DVU0279 0.90 -1.1 323,925 + -3.3 323,945 - -0.7 324,032 + +0.3 324,057 + -1.6 324,065 - +0.7 324,074 + +0.5 324,077 - -0.1 324,079 + -0.8 324,082 - +0.9 324,169 - -0.1 324,183 + -1.1 324,183 + -0.4 324,183 + -1.1 324,191 - -0.6 324,191 - -0.6 324,325 - -0.4 324,561 + wbaZ-2 DVU0280 0.14 +2.9 324,640 + wbaZ-2 DVU0280 0.21 +4.9 324,712 + wbaZ-2 DVU0280 0.28 +4.9 324,712 + wbaZ-2 DVU0280 0.28 +4.9 324,824 - wbaZ-2 DVU0280 0.37 +2.9 324,868 + wbaZ-2 DVU0280 0.41 +2.9 324,982 + wbaZ-2 DVU0280 0.51 +2.4
Or see this region's nucleotide sequence