Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potA and DVU0099 are separated by 417 nucleotides DVU0099 and DVU0100 are separated by 296 nucleotides
DVU0098: potA - polyamine ABC transporter, ATP-binding protein (TIGR), at 128,366 to 129,472
potA
DVU0099: DVU0099 - TonB domain protein (TIGR), at 129,890 to 131,050
DVU0099
DVU0100: DVU0100 - TonB-dependent receptor (TIGR), at 131,347 to 133,296
DVU0100
Position (kb) 129
130
131
132 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 128.905 kb on - strand, within potA at 128.905 kb on - strand, within potA at 128.942 kb on - strand, within potA at 128.969 kb on - strand, within potA at 128.981 kb on - strand, within potA at 128.983 kb on - strand, within potA at 129.011 kb on + strand, within potA at 129.051 kb on - strand, within potA at 129.251 kb on + strand, within potA at 129.322 kb on + strand, within potA at 129.351 kb on + strand, within potA at 129.426 kb on - strand at 129.445 kb on + strand at 129.512 kb on - strand at 129.683 kb on + strand at 129.744 kb on - strand at 129.747 kb on + strand at 129.769 kb on + strand at 129.816 kb on + strand at 129.940 kb on - strand at 129.988 kb on - strand at 130.043 kb on - strand, within DVU0099 at 130.069 kb on + strand, within DVU0099 at 130.115 kb on - strand, within DVU0099 at 130.172 kb on + strand, within DVU0099 at 130.177 kb on + strand, within DVU0099 at 130.177 kb on + strand, within DVU0099 at 130.184 kb on + strand, within DVU0099 at 130.229 kb on + strand, within DVU0099 at 130.237 kb on - strand, within DVU0099 at 130.237 kb on - strand, within DVU0099 at 130.239 kb on + strand, within DVU0099 at 130.239 kb on + strand, within DVU0099 at 130.247 kb on - strand, within DVU0099 at 130.366 kb on - strand, within DVU0099 at 130.635 kb on - strand, within DVU0099 at 130.635 kb on - strand, within DVU0099 at 130.635 kb on - strand, within DVU0099 at 130.697 kb on + strand, within DVU0099 at 130.697 kb on + strand, within DVU0099 at 130.697 kb on + strand, within DVU0099 at 130.697 kb on + strand, within DVU0099 at 130.702 kb on + strand, within DVU0099 at 130.705 kb on - strand, within DVU0099 at 130.705 kb on - strand, within DVU0099 at 130.710 kb on - strand, within DVU0099 at 130.710 kb on - strand, within DVU0099 at 130.710 kb on - strand, within DVU0099 at 130.720 kb on + strand, within DVU0099 at 130.721 kb on - strand, within DVU0099 at 130.767 kb on + strand, within DVU0099 at 130.772 kb on + strand, within DVU0099 at 130.837 kb on + strand, within DVU0099 at 130.863 kb on + strand, within DVU0099 at 130.878 kb on - strand, within DVU0099 at 130.931 kb on - strand, within DVU0099 at 130.933 kb on + strand, within DVU0099 at 130.995 kb on - strand at 131.013 kb on + strand at 131.068 kb on - strand at 131.173 kb on + strand at 131.181 kb on - strand at 131.194 kb on + strand at 131.388 kb on + strand at 131.396 kb on - strand at 131.512 kb on + strand at 131.512 kb on + strand at 131.513 kb on - strand at 131.539 kb on - strand at 131.557 kb on + strand, within DVU0100 at 131.565 kb on + strand, within DVU0100 at 131.603 kb on + strand, within DVU0100 at 131.680 kb on - strand, within DVU0100 at 131.732 kb on - strand, within DVU0100 at 131.732 kb on - strand, within DVU0100 at 131.734 kb on + strand, within DVU0100 at 131.737 kb on - strand, within DVU0100 at 131.742 kb on - strand, within DVU0100 at 131.781 kb on + strand, within DVU0100 at 131.789 kb on - strand, within DVU0100 at 131.793 kb on + strand, within DVU0100 at 131.793 kb on + strand, within DVU0100 at 131.871 kb on - strand, within DVU0100 at 131.935 kb on - strand, within DVU0100 at 131.949 kb on + strand, within DVU0100 at 131.957 kb on - strand, within DVU0100 at 131.976 kb on + strand, within DVU0100 at 131.976 kb on + strand, within DVU0100 at 131.976 kb on + strand, within DVU0100 at 132.047 kb on + strand, within DVU0100
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 128,905 - potA DVU0098 0.49 +1.9 128,905 - potA DVU0098 0.49 +0.8 128,942 - potA DVU0098 0.52 -1.4 128,969 - potA DVU0098 0.54 +2.5 128,981 - potA DVU0098 0.56 -1.7 128,983 - potA DVU0098 0.56 +1.0 129,011 + potA DVU0098 0.58 +1.3 129,051 - potA DVU0098 0.62 -0.4 129,251 + potA DVU0098 0.80 +2.3 129,322 + potA DVU0098 0.86 -0.2 129,351 + potA DVU0098 0.89 -0.9 129,426 - -2.5 129,445 + -1.8 129,512 - -0.3 129,683 + -1.1 129,744 - -0.2 129,747 + -0.6 129,769 + -0.7 129,816 + +2.3 129,940 - -0.7 129,988 - +3.1 130,043 - DVU0099 0.13 -0.2 130,069 + DVU0099 0.15 -1.2 130,115 - DVU0099 0.19 -2.2 130,172 + DVU0099 0.24 -0.9 130,177 + DVU0099 0.25 +3.3 130,177 + DVU0099 0.25 +0.5 130,184 + DVU0099 0.25 +1.6 130,229 + DVU0099 0.29 -0.7 130,237 - DVU0099 0.30 -1.2 130,237 - DVU0099 0.30 -1.0 130,239 + DVU0099 0.30 -1.2 130,239 + DVU0099 0.30 -2.0 130,247 - DVU0099 0.31 -1.0 130,366 - DVU0099 0.41 -2.2 130,635 - DVU0099 0.64 +1.7 130,635 - DVU0099 0.64 +2.9 130,635 - DVU0099 0.64 -0.7 130,697 + DVU0099 0.70 -0.9 130,697 + DVU0099 0.70 -2.0 130,697 + DVU0099 0.70 -1.9 130,697 + DVU0099 0.70 -0.4 130,702 + DVU0099 0.70 +0.5 130,705 - DVU0099 0.70 -2.7 130,705 - DVU0099 0.70 -2.1 130,710 - DVU0099 0.71 +3.9 130,710 - DVU0099 0.71 +2.1 130,710 - DVU0099 0.71 -0.5 130,720 + DVU0099 0.71 -1.2 130,721 - DVU0099 0.72 -2.0 130,767 + DVU0099 0.76 -0.5 130,772 + DVU0099 0.76 -1.4 130,837 + DVU0099 0.82 +1.9 130,863 + DVU0099 0.84 -1.2 130,878 - DVU0099 0.85 -2.1 130,931 - DVU0099 0.90 +3.3 130,933 + DVU0099 0.90 -1.0 130,995 - +3.1 131,013 + -0.2 131,068 - -0.4 131,173 + +0.8 131,181 - -1.6 131,194 + +1.5 131,388 + -2.1 131,396 - -0.7 131,512 + -0.7 131,512 + +0.7 131,513 - -0.3 131,539 - -1.0 131,557 + DVU0100 0.11 +1.0 131,565 + DVU0100 0.11 -1.8 131,603 + DVU0100 0.13 +1.2 131,680 - DVU0100 0.17 -0.4 131,732 - DVU0100 0.20 -1.2 131,732 - DVU0100 0.20 -0.6 131,734 + DVU0100 0.20 -1.2 131,737 - DVU0100 0.20 +0.5 131,742 - DVU0100 0.20 -0.4 131,781 + DVU0100 0.22 -0.7 131,789 - DVU0100 0.23 -1.2 131,793 + DVU0100 0.23 -0.0 131,793 + DVU0100 0.23 -0.3 131,871 - DVU0100 0.27 -0.5 131,935 - DVU0100 0.30 -1.6 131,949 + DVU0100 0.31 -1.3 131,957 - DVU0100 0.31 +2.9 131,976 + DVU0100 0.32 +0.5 131,976 + DVU0100 0.32 -1.3 131,976 + DVU0100 0.32 +1.2 132,047 + DVU0100 0.36 -1.2
Or see this region's nucleotide sequence