Strain Fitness in Desulfovibrio vulgaris Hildenborough JW710 around DVU3065

Experiment: MoLS4 with L-Cysteine as nutrient 10mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDVU3064 and DVU3065 are separated by 127 nucleotidesDVU3065 and DVU3066 are separated by 14 nucleotidesDVU3066 and hypF are separated by 253 nucleotides DVU3064: DVU3064 - sensory box/GGDEF domain/EAL domain protein (TIGR), at 3,198,740 to 3,200,470 DVU3064 DVU3065: DVU3065 - AMP-binding enzyme family protein (TIGR), at 3,200,598 to 3,202,250 DVU3065 DVU3066: DVU3066 - DNA-binding protein (TIGR), at 3,202,265 to 3,202,834 DVU3066 DVU3067: hypF - [NiFe] hydrogenase maturation protein HypF (TIGR), at 3,203,088 to 3,205,448 hypF Position (kb) 3200 3201 3202 3203Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 3199.636 kb on + strand, within DVU3064at 3199.709 kb on - strand, within DVU3064at 3199.719 kb on - strand, within DVU3064at 3199.819 kb on + strand, within DVU3064at 3200.001 kb on + strand, within DVU3064at 3200.033 kb on - strand, within DVU3064at 3200.053 kb on - strand, within DVU3064at 3200.082 kb on + strand, within DVU3064at 3200.082 kb on + strand, within DVU3064at 3200.090 kb on - strand, within DVU3064at 3200.143 kb on - strand, within DVU3064at 3200.287 kb on + strand, within DVU3064at 3200.295 kb on - strand, within DVU3064at 3200.331 kb on + strandat 3200.339 kb on - strandat 3200.361 kb on + strandat 3200.373 kb on + strandat 3200.441 kb on + strandat 3200.478 kb on + strandat 3200.478 kb on + strandat 3200.505 kb on + strandat 3200.517 kb on + strandat 3200.532 kb on + strandat 3200.553 kb on + strandat 3200.580 kb on - strandat 3200.594 kb on + strandat 3200.753 kb on + strandat 3200.813 kb on + strand, within DVU3065at 3200.890 kb on + strand, within DVU3065at 3200.968 kb on + strand, within DVU3065at 3200.993 kb on + strand, within DVU3065at 3201.015 kb on + strand, within DVU3065at 3201.018 kb on - strand, within DVU3065at 3201.131 kb on + strand, within DVU3065at 3201.167 kb on - strand, within DVU3065at 3201.192 kb on - strand, within DVU3065at 3201.292 kb on + strand, within DVU3065at 3201.370 kb on + strand, within DVU3065at 3201.398 kb on + strand, within DVU3065at 3201.401 kb on - strand, within DVU3065at 3201.442 kb on + strand, within DVU3065at 3201.442 kb on + strand, within DVU3065at 3201.450 kb on - strand, within DVU3065at 3201.450 kb on - strand, within DVU3065at 3201.517 kb on + strand, within DVU3065at 3201.525 kb on - strand, within DVU3065at 3201.652 kb on - strand, within DVU3065at 3201.736 kb on - strand, within DVU3065at 3201.754 kb on + strand, within DVU3065at 3201.764 kb on - strand, within DVU3065at 3201.878 kb on + strand, within DVU3065at 3201.962 kb on + strand, within DVU3065at 3201.976 kb on - strand, within DVU3065at 3201.979 kb on + strand, within DVU3065at 3201.982 kb on - strand, within DVU3065at 3201.992 kb on - strand, within DVU3065at 3202.002 kb on - strand, within DVU3065at 3202.035 kb on - strand, within DVU3065at 3202.081 kb on - strand, within DVU3065at 3202.086 kb on + strandat 3202.096 kb on - strandat 3202.107 kb on + strandat 3202.159 kb on + strandat 3202.182 kb on + strandat 3202.216 kb on - strandat 3202.228 kb on - strandat 3202.244 kb on + strandat 3202.252 kb on - strandat 3202.287 kb on + strandat 3202.287 kb on + strandat 3202.295 kb on - strandat 3202.295 kb on - strandat 3202.295 kb on - strandat 3202.295 kb on - strandat 3202.305 kb on - strandat 3202.354 kb on - strand, within DVU3066at 3202.356 kb on + strand, within DVU3066at 3202.424 kb on + strand, within DVU3066at 3202.473 kb on - strand, within DVU3066at 3202.476 kb on + strand, within DVU3066at 3202.523 kb on - strand, within DVU3066at 3202.554 kb on - strand, within DVU3066at 3202.647 kb on + strand, within DVU3066at 3202.655 kb on - strand, within DVU3066at 3202.692 kb on - strand, within DVU3066at 3202.701 kb on + strand, within DVU3066at 3202.702 kb on + strand, within DVU3066at 3202.710 kb on - strand, within DVU3066at 3202.783 kb on - strandat 3202.795 kb on - strandat 3202.886 kb on - strandat 3202.890 kb on - strandat 3203.178 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM
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3,199,636 + DVU3064 0.52 -1.0
3,199,709 - DVU3064 0.56 +2.2
3,199,719 - DVU3064 0.57 -1.8
3,199,819 + DVU3064 0.62 +2.4
3,200,001 + DVU3064 0.73 -1.9
3,200,033 - DVU3064 0.75 -0.1
3,200,053 - DVU3064 0.76 +1.1
3,200,082 + DVU3064 0.78 +2.2
3,200,082 + DVU3064 0.78 +1.6
3,200,090 - DVU3064 0.78 +2.4
3,200,143 - DVU3064 0.81 +2.6
3,200,287 + DVU3064 0.89 -1.0
3,200,295 - DVU3064 0.90 -1.2
3,200,331 + -1.2
3,200,339 - +3.0
3,200,361 + +0.7
3,200,373 + -1.6
3,200,441 + +0.6
3,200,478 + -0.8
3,200,478 + +1.0
3,200,505 + +1.0
3,200,517 + +4.4
3,200,532 + -0.3
3,200,553 + -2.1
3,200,580 - -0.7
3,200,594 + +1.2
3,200,753 + -0.2
3,200,813 + DVU3065 0.13 +0.6
3,200,890 + DVU3065 0.18 +0.3
3,200,968 + DVU3065 0.22 +3.4
3,200,993 + DVU3065 0.24 -3.1
3,201,015 + DVU3065 0.25 -1.1
3,201,018 - DVU3065 0.25 -0.7
3,201,131 + DVU3065 0.32 -0.3
3,201,167 - DVU3065 0.34 +0.3
3,201,192 - DVU3065 0.36 +0.4
3,201,292 + DVU3065 0.42 -0.2
3,201,370 + DVU3065 0.47 +1.6
3,201,398 + DVU3065 0.48 -1.2
3,201,401 - DVU3065 0.49 +0.0
3,201,442 + DVU3065 0.51 -1.0
3,201,442 + DVU3065 0.51 +0.4
3,201,450 - DVU3065 0.52 +0.3
3,201,450 - DVU3065 0.52 -0.9
3,201,517 + DVU3065 0.56 -0.7
3,201,525 - DVU3065 0.56 +0.9
3,201,652 - DVU3065 0.64 -0.1
3,201,736 - DVU3065 0.69 +3.0
3,201,754 + DVU3065 0.70 -1.5
3,201,764 - DVU3065 0.71 +2.4
3,201,878 + DVU3065 0.77 +1.1
3,201,962 + DVU3065 0.83 +0.6
3,201,976 - DVU3065 0.83 +2.2
3,201,979 + DVU3065 0.84 -2.4
3,201,982 - DVU3065 0.84 +2.1
3,201,992 - DVU3065 0.84 +3.0
3,202,002 - DVU3065 0.85 +1.1
3,202,035 - DVU3065 0.87 +0.4
3,202,081 - DVU3065 0.90 -0.1
3,202,086 + +5.0
3,202,096 - -0.0
3,202,107 + -0.6
3,202,159 + -0.7
3,202,182 + +0.1
3,202,216 - +0.1
3,202,228 - -0.4
3,202,244 + +1.3
3,202,252 - +2.2
3,202,287 + +0.3
3,202,287 + -0.9
3,202,295 - +1.8
3,202,295 - +2.6
3,202,295 - -1.8
3,202,295 - -0.9
3,202,305 - +0.5
3,202,354 - DVU3066 0.16 -0.3
3,202,356 + DVU3066 0.16 +0.7
3,202,424 + DVU3066 0.28 +0.1
3,202,473 - DVU3066 0.36 -1.3
3,202,476 + DVU3066 0.37 +0.8
3,202,523 - DVU3066 0.45 +2.0
3,202,554 - DVU3066 0.51 -1.1
3,202,647 + DVU3066 0.67 -0.4
3,202,655 - DVU3066 0.68 +1.6
3,202,692 - DVU3066 0.75 -1.6
3,202,701 + DVU3066 0.76 -0.8
3,202,702 + DVU3066 0.77 +0.5
3,202,710 - DVU3066 0.78 +3.0
3,202,783 - +1.4
3,202,795 - -0.2
3,202,886 - -0.4
3,202,890 - -0.5
3,203,178 + +3.4

Or see this region's nucleotide sequence