Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3064 and DVU3065 are separated by 127 nucleotides DVU3065 and DVU3066 are separated by 14 nucleotides DVU3066 and hypF are separated by 253 nucleotides
DVU3064: DVU3064 - sensory box/GGDEF domain/EAL domain protein (TIGR), at 3,198,740 to 3,200,470
DVU3064
DVU3065: DVU3065 - AMP-binding enzyme family protein (TIGR), at 3,200,598 to 3,202,250
DVU3065
DVU3066: DVU3066 - DNA-binding protein (TIGR), at 3,202,265 to 3,202,834
DVU3066
DVU3067: hypF - [NiFe] hydrogenase maturation protein HypF (TIGR), at 3,203,088 to 3,205,448
hypF
Position (kb)
3200
3201
3202
3203 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3199.636 kb on + strand, within DVU3064 at 3199.709 kb on - strand, within DVU3064 at 3199.719 kb on - strand, within DVU3064 at 3199.819 kb on + strand, within DVU3064 at 3200.001 kb on + strand, within DVU3064 at 3200.033 kb on - strand, within DVU3064 at 3200.053 kb on - strand, within DVU3064 at 3200.082 kb on + strand, within DVU3064 at 3200.082 kb on + strand, within DVU3064 at 3200.090 kb on - strand, within DVU3064 at 3200.143 kb on - strand, within DVU3064 at 3200.287 kb on + strand, within DVU3064 at 3200.295 kb on - strand, within DVU3064 at 3200.331 kb on + strand at 3200.339 kb on - strand at 3200.361 kb on + strand at 3200.373 kb on + strand at 3200.441 kb on + strand at 3200.478 kb on + strand at 3200.478 kb on + strand at 3200.505 kb on + strand at 3200.517 kb on + strand at 3200.532 kb on + strand at 3200.553 kb on + strand at 3200.580 kb on - strand at 3200.594 kb on + strand at 3200.753 kb on + strand at 3200.813 kb on + strand, within DVU3065 at 3200.890 kb on + strand, within DVU3065 at 3200.968 kb on + strand, within DVU3065 at 3200.993 kb on + strand, within DVU3065 at 3201.015 kb on + strand, within DVU3065 at 3201.018 kb on - strand, within DVU3065 at 3201.131 kb on + strand, within DVU3065 at 3201.167 kb on - strand, within DVU3065 at 3201.192 kb on - strand, within DVU3065 at 3201.292 kb on + strand, within DVU3065 at 3201.370 kb on + strand, within DVU3065 at 3201.398 kb on + strand, within DVU3065 at 3201.401 kb on - strand, within DVU3065 at 3201.442 kb on + strand, within DVU3065 at 3201.442 kb on + strand, within DVU3065 at 3201.450 kb on - strand, within DVU3065 at 3201.450 kb on - strand, within DVU3065 at 3201.517 kb on + strand, within DVU3065 at 3201.525 kb on - strand, within DVU3065 at 3201.652 kb on - strand, within DVU3065 at 3201.736 kb on - strand, within DVU3065 at 3201.754 kb on + strand, within DVU3065 at 3201.764 kb on - strand, within DVU3065 at 3201.878 kb on + strand, within DVU3065 at 3201.962 kb on + strand, within DVU3065 at 3201.976 kb on - strand, within DVU3065 at 3201.979 kb on + strand, within DVU3065 at 3201.982 kb on - strand, within DVU3065 at 3201.992 kb on - strand, within DVU3065 at 3202.002 kb on - strand, within DVU3065 at 3202.035 kb on - strand, within DVU3065 at 3202.081 kb on - strand, within DVU3065 at 3202.086 kb on + strand at 3202.096 kb on - strand at 3202.107 kb on + strand at 3202.159 kb on + strand at 3202.182 kb on + strand at 3202.216 kb on - strand at 3202.228 kb on - strand at 3202.244 kb on + strand at 3202.252 kb on - strand at 3202.287 kb on + strand at 3202.287 kb on + strand at 3202.295 kb on - strand at 3202.295 kb on - strand at 3202.295 kb on - strand at 3202.295 kb on - strand at 3202.305 kb on - strand at 3202.354 kb on - strand, within DVU3066 at 3202.356 kb on + strand, within DVU3066 at 3202.424 kb on + strand, within DVU3066 at 3202.473 kb on - strand, within DVU3066 at 3202.476 kb on + strand, within DVU3066 at 3202.523 kb on - strand, within DVU3066 at 3202.554 kb on - strand, within DVU3066 at 3202.647 kb on + strand, within DVU3066 at 3202.655 kb on - strand, within DVU3066 at 3202.692 kb on - strand, within DVU3066 at 3202.701 kb on + strand, within DVU3066 at 3202.702 kb on + strand, within DVU3066 at 3202.710 kb on - strand, within DVU3066 at 3202.783 kb on - strand at 3202.795 kb on - strand at 3202.886 kb on - strand at 3202.890 kb on - strand at 3203.178 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 3,199,636 + DVU3064 0.52 -1.0 3,199,709 - DVU3064 0.56 +2.2 3,199,719 - DVU3064 0.57 -1.8 3,199,819 + DVU3064 0.62 +2.4 3,200,001 + DVU3064 0.73 -1.9 3,200,033 - DVU3064 0.75 -0.1 3,200,053 - DVU3064 0.76 +1.1 3,200,082 + DVU3064 0.78 +2.2 3,200,082 + DVU3064 0.78 +1.6 3,200,090 - DVU3064 0.78 +2.4 3,200,143 - DVU3064 0.81 +2.6 3,200,287 + DVU3064 0.89 -1.0 3,200,295 - DVU3064 0.90 -1.2 3,200,331 + -1.2 3,200,339 - +3.0 3,200,361 + +0.7 3,200,373 + -1.6 3,200,441 + +0.6 3,200,478 + -0.8 3,200,478 + +1.0 3,200,505 + +1.0 3,200,517 + +4.4 3,200,532 + -0.3 3,200,553 + -2.1 3,200,580 - -0.7 3,200,594 + +1.2 3,200,753 + -0.2 3,200,813 + DVU3065 0.13 +0.6 3,200,890 + DVU3065 0.18 +0.3 3,200,968 + DVU3065 0.22 +3.4 3,200,993 + DVU3065 0.24 -3.1 3,201,015 + DVU3065 0.25 -1.1 3,201,018 - DVU3065 0.25 -0.7 3,201,131 + DVU3065 0.32 -0.3 3,201,167 - DVU3065 0.34 +0.3 3,201,192 - DVU3065 0.36 +0.4 3,201,292 + DVU3065 0.42 -0.2 3,201,370 + DVU3065 0.47 +1.6 3,201,398 + DVU3065 0.48 -1.2 3,201,401 - DVU3065 0.49 +0.0 3,201,442 + DVU3065 0.51 -1.0 3,201,442 + DVU3065 0.51 +0.4 3,201,450 - DVU3065 0.52 +0.3 3,201,450 - DVU3065 0.52 -0.9 3,201,517 + DVU3065 0.56 -0.7 3,201,525 - DVU3065 0.56 +0.9 3,201,652 - DVU3065 0.64 -0.1 3,201,736 - DVU3065 0.69 +3.0 3,201,754 + DVU3065 0.70 -1.5 3,201,764 - DVU3065 0.71 +2.4 3,201,878 + DVU3065 0.77 +1.1 3,201,962 + DVU3065 0.83 +0.6 3,201,976 - DVU3065 0.83 +2.2 3,201,979 + DVU3065 0.84 -2.4 3,201,982 - DVU3065 0.84 +2.1 3,201,992 - DVU3065 0.84 +3.0 3,202,002 - DVU3065 0.85 +1.1 3,202,035 - DVU3065 0.87 +0.4 3,202,081 - DVU3065 0.90 -0.1 3,202,086 + +5.0 3,202,096 - -0.0 3,202,107 + -0.6 3,202,159 + -0.7 3,202,182 + +0.1 3,202,216 - +0.1 3,202,228 - -0.4 3,202,244 + +1.3 3,202,252 - +2.2 3,202,287 + +0.3 3,202,287 + -0.9 3,202,295 - +1.8 3,202,295 - +2.6 3,202,295 - -1.8 3,202,295 - -0.9 3,202,305 - +0.5 3,202,354 - DVU3066 0.16 -0.3 3,202,356 + DVU3066 0.16 +0.7 3,202,424 + DVU3066 0.28 +0.1 3,202,473 - DVU3066 0.36 -1.3 3,202,476 + DVU3066 0.37 +0.8 3,202,523 - DVU3066 0.45 +2.0 3,202,554 - DVU3066 0.51 -1.1 3,202,647 + DVU3066 0.67 -0.4 3,202,655 - DVU3066 0.68 +1.6 3,202,692 - DVU3066 0.75 -1.6 3,202,701 + DVU3066 0.76 -0.8 3,202,702 + DVU3066 0.77 +0.5 3,202,710 - DVU3066 0.78 +3.0 3,202,783 - +1.4 3,202,795 - -0.2 3,202,886 - -0.4 3,202,890 - -0.5 3,203,178 + +3.4
Or see this region's nucleotide sequence