Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2490 and DVU2491 are separated by 5 nucleotides DVU2491 and trpF-2 are separated by 85 nucleotides
DVU2490: DVU2490 - Histidinol phosphatase (Natalia Ivanova), at 2,601,007 to 2,601,831
DVU2490
DVU2491: DVU2491 - ABC transporter, ATP-binding protein (TIGR), at 2,601,837 to 2,603,735
DVU2491
DVU2492: trpF-2 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 2,603,821 to 2,604,747
trpF-2
Position (kb)
2601
2602
2603
2604 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2600.843 kb on + strand at 2600.848 kb on + strand at 2600.851 kb on - strand at 2600.922 kb on - strand at 2600.969 kb on - strand at 2600.979 kb on + strand at 2601.927 kb on - strand at 2601.936 kb on + strand at 2601.992 kb on + strand at 2602.079 kb on - strand, within DVU2491 at 2602.081 kb on + strand, within DVU2491 at 2602.104 kb on + strand, within DVU2491 at 2602.223 kb on + strand, within DVU2491 at 2602.250 kb on + strand, within DVU2491 at 2602.343 kb on + strand, within DVU2491 at 2602.389 kb on + strand, within DVU2491 at 2602.389 kb on + strand, within DVU2491 at 2602.458 kb on + strand, within DVU2491 at 2602.467 kb on + strand, within DVU2491 at 2602.485 kb on - strand, within DVU2491 at 2602.487 kb on - strand, within DVU2491 at 2602.647 kb on - strand, within DVU2491 at 2602.699 kb on + strand, within DVU2491 at 2602.707 kb on - strand, within DVU2491 at 2602.714 kb on - strand, within DVU2491 at 2602.735 kb on + strand, within DVU2491 at 2602.735 kb on + strand, within DVU2491 at 2602.756 kb on + strand, within DVU2491 at 2602.761 kb on + strand, within DVU2491 at 2602.769 kb on - strand, within DVU2491 at 2602.814 kb on + strand, within DVU2491 at 2602.897 kb on - strand, within DVU2491 at 2602.918 kb on + strand, within DVU2491 at 2602.921 kb on - strand, within DVU2491 at 2603.021 kb on + strand, within DVU2491 at 2603.058 kb on + strand, within DVU2491 at 2603.072 kb on + strand, within DVU2491 at 2603.076 kb on - strand, within DVU2491 at 2603.199 kb on + strand, within DVU2491 at 2603.322 kb on + strand, within DVU2491 at 2603.323 kb on - strand, within DVU2491 at 2603.323 kb on - strand, within DVU2491 at 2603.335 kb on + strand, within DVU2491 at 2603.387 kb on - strand, within DVU2491 at 2603.387 kb on - strand, within DVU2491 at 2603.429 kb on + strand, within DVU2491 at 2603.448 kb on + strand, within DVU2491 at 2603.545 kb on + strand, within DVU2491 at 2603.553 kb on - strand at 2603.563 kb on - strand at 2603.597 kb on - strand at 2603.610 kb on - strand at 2603.694 kb on - strand at 2603.931 kb on + strand, within trpF-2 at 2603.946 kb on - strand, within trpF-2 at 2604.045 kb on + strand, within trpF-2 at 2604.144 kb on - strand, within trpF-2 at 2604.213 kb on - strand, within trpF-2 at 2604.232 kb on + strand, within trpF-2 at 2604.270 kb on + strand, within trpF-2 at 2604.275 kb on + strand, within trpF-2 at 2604.288 kb on - strand, within trpF-2 at 2604.377 kb on + strand, within trpF-2 at 2604.377 kb on + strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.431 kb on - strand, within trpF-2 at 2604.460 kb on + strand, within trpF-2 at 2604.530 kb on - strand, within trpF-2 at 2604.541 kb on + strand, within trpF-2 at 2604.621 kb on + strand, within trpF-2 at 2604.662 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 2,600,843 + +2.3 2,600,848 + -1.1 2,600,851 - +0.5 2,600,922 - +1.7 2,600,969 - +1.7 2,600,979 + -0.0 2,601,927 - +1.4 2,601,936 + -1.2 2,601,992 + +1.6 2,602,079 - DVU2491 0.13 -0.5 2,602,081 + DVU2491 0.13 +2.3 2,602,104 + DVU2491 0.14 +1.9 2,602,223 + DVU2491 0.20 -0.2 2,602,250 + DVU2491 0.22 -1.2 2,602,343 + DVU2491 0.27 +1.6 2,602,389 + DVU2491 0.29 -1.1 2,602,389 + DVU2491 0.29 -0.2 2,602,458 + DVU2491 0.33 -1.1 2,602,467 + DVU2491 0.33 +1.4 2,602,485 - DVU2491 0.34 -0.7 2,602,487 - DVU2491 0.34 +1.4 2,602,647 - DVU2491 0.43 +0.9 2,602,699 + DVU2491 0.45 -0.5 2,602,707 - DVU2491 0.46 -2.2 2,602,714 - DVU2491 0.46 +0.7 2,602,735 + DVU2491 0.47 +0.3 2,602,735 + DVU2491 0.47 -0.8 2,602,756 + DVU2491 0.48 +1.3 2,602,761 + DVU2491 0.49 +0.2 2,602,769 - DVU2491 0.49 +0.2 2,602,814 + DVU2491 0.51 -0.7 2,602,897 - DVU2491 0.56 -0.6 2,602,918 + DVU2491 0.57 -1.2 2,602,921 - DVU2491 0.57 -0.6 2,603,021 + DVU2491 0.62 +0.3 2,603,058 + DVU2491 0.64 -0.3 2,603,072 + DVU2491 0.65 -0.9 2,603,076 - DVU2491 0.65 +3.1 2,603,199 + DVU2491 0.72 +0.5 2,603,322 + DVU2491 0.78 -0.1 2,603,323 - DVU2491 0.78 +0.0 2,603,323 - DVU2491 0.78 +0.2 2,603,335 + DVU2491 0.79 +2.0 2,603,387 - DVU2491 0.82 +1.0 2,603,387 - DVU2491 0.82 -1.3 2,603,429 + DVU2491 0.84 +0.5 2,603,448 + DVU2491 0.85 -0.3 2,603,545 + DVU2491 0.90 +1.2 2,603,553 - -1.0 2,603,563 - -0.8 2,603,597 - -0.5 2,603,610 - -0.6 2,603,694 - +0.5 2,603,931 + trpF-2 DVU2492 0.12 +0.0 2,603,946 - trpF-2 DVU2492 0.13 +2.5 2,604,045 + trpF-2 DVU2492 0.24 -1.1 2,604,144 - trpF-2 DVU2492 0.35 +3.1 2,604,213 - trpF-2 DVU2492 0.42 -1.5 2,604,232 + trpF-2 DVU2492 0.44 +0.7 2,604,270 + trpF-2 DVU2492 0.48 +2.3 2,604,275 + trpF-2 DVU2492 0.49 -1.3 2,604,288 - trpF-2 DVU2492 0.50 +1.5 2,604,377 + trpF-2 DVU2492 0.60 -1.3 2,604,377 + trpF-2 DVU2492 0.60 -0.8 2,604,385 - trpF-2 DVU2492 0.61 +0.4 2,604,385 - trpF-2 DVU2492 0.61 +2.5 2,604,385 - trpF-2 DVU2492 0.61 -1.6 2,604,385 - trpF-2 DVU2492 0.61 -2.0 2,604,385 - trpF-2 DVU2492 0.61 +0.3 2,604,431 - trpF-2 DVU2492 0.66 +1.9 2,604,460 + trpF-2 DVU2492 0.69 +0.7 2,604,530 - trpF-2 DVU2492 0.76 -1.2 2,604,541 + trpF-2 DVU2492 0.78 +0.1 2,604,621 + trpF-2 DVU2492 0.86 +0.5 2,604,662 + -1.3
Or see this region's nucleotide sequence