Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2295 and mtgA are separated by 427 nucleotides mtgA and DVU2297 are separated by 212 nucleotides
DVU2295: DVU2295 - methyl-accepting chemotaxis protein (TIGR), at 2,387,414 to 2,389,744
DVU2295
DVU2296: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (TIGR), at 2,390,172 to 2,390,918
mtgA
DVU2297: DVU2297 - glycine/betaine/L-proline ABC transporter, periplasmic-binding protein (TIGR), at 2,391,131 to 2,391,988
DVU2297
Position (kb)
2390
2391 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2389.176 kb on + strand, within DVU2295 at 2389.189 kb on - strand, within DVU2295 at 2389.204 kb on - strand, within DVU2295 at 2389.243 kb on + strand, within DVU2295 at 2389.243 kb on + strand, within DVU2295 at 2389.266 kb on - strand, within DVU2295 at 2389.309 kb on - strand, within DVU2295 at 2389.346 kb on + strand, within DVU2295 at 2389.365 kb on + strand, within DVU2295 at 2389.403 kb on + strand, within DVU2295 at 2389.403 kb on + strand, within DVU2295 at 2389.411 kb on - strand, within DVU2295 at 2389.411 kb on - strand, within DVU2295 at 2389.445 kb on - strand, within DVU2295 at 2389.467 kb on - strand, within DVU2295 at 2389.573 kb on + strand at 2389.660 kb on - strand at 2389.881 kb on + strand at 2390.011 kb on - strand at 2390.052 kb on + strand at 2390.125 kb on - strand at 2390.138 kb on - strand at 2390.283 kb on + strand, within mtgA at 2390.283 kb on + strand, within mtgA at 2390.293 kb on + strand, within mtgA at 2390.306 kb on - strand, within mtgA at 2390.373 kb on + strand, within mtgA at 2390.373 kb on + strand, within mtgA at 2390.425 kb on + strand, within mtgA at 2390.437 kb on - strand, within mtgA at 2390.464 kb on - strand, within mtgA at 2390.569 kb on - strand, within mtgA at 2390.612 kb on + strand, within mtgA at 2390.679 kb on - strand, within mtgA at 2390.686 kb on + strand, within mtgA at 2390.759 kb on + strand, within mtgA at 2390.785 kb on + strand, within mtgA at 2390.788 kb on - strand, within mtgA at 2390.917 kb on + strand at 2390.931 kb on + strand at 2390.931 kb on + strand at 2390.944 kb on - strand at 2390.956 kb on + strand at 2390.997 kb on - strand at 2391.006 kb on - strand at 2391.046 kb on + strand at 2391.046 kb on + strand at 2391.054 kb on - strand at 2391.165 kb on - strand at 2391.226 kb on + strand, within DVU2297 at 2391.242 kb on - strand, within DVU2297 at 2391.291 kb on + strand, within DVU2297 at 2391.305 kb on - strand, within DVU2297 at 2391.314 kb on + strand, within DVU2297 at 2391.777 kb on + strand, within DVU2297 at 2391.846 kb on + strand, within DVU2297 at 2391.900 kb on + strand, within DVU2297
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 2,389,176 + DVU2295 0.76 +3.1 2,389,189 - DVU2295 0.76 +2.2 2,389,204 - DVU2295 0.77 -0.5 2,389,243 + DVU2295 0.78 -1.0 2,389,243 + DVU2295 0.78 +1.8 2,389,266 - DVU2295 0.79 -0.6 2,389,309 - DVU2295 0.81 -1.0 2,389,346 + DVU2295 0.83 +1.1 2,389,365 + DVU2295 0.84 -2.5 2,389,403 + DVU2295 0.85 +0.9 2,389,403 + DVU2295 0.85 -0.1 2,389,411 - DVU2295 0.86 -0.7 2,389,411 - DVU2295 0.86 +0.1 2,389,445 - DVU2295 0.87 -0.5 2,389,467 - DVU2295 0.88 -0.0 2,389,573 + +3.5 2,389,660 - +0.8 2,389,881 + -0.1 2,390,011 - -0.3 2,390,052 + -1.1 2,390,125 - -1.9 2,390,138 - -1.3 2,390,283 + mtgA DVU2296 0.15 -0.1 2,390,283 + mtgA DVU2296 0.15 +1.3 2,390,293 + mtgA DVU2296 0.16 +0.1 2,390,306 - mtgA DVU2296 0.18 -0.8 2,390,373 + mtgA DVU2296 0.27 -0.9 2,390,373 + mtgA DVU2296 0.27 -0.8 2,390,425 + mtgA DVU2296 0.34 +2.3 2,390,437 - mtgA DVU2296 0.35 +0.9 2,390,464 - mtgA DVU2296 0.39 +3.5 2,390,569 - mtgA DVU2296 0.53 +0.2 2,390,612 + mtgA DVU2296 0.59 +0.8 2,390,679 - mtgA DVU2296 0.68 -0.4 2,390,686 + mtgA DVU2296 0.69 -1.1 2,390,759 + mtgA DVU2296 0.79 +2.4 2,390,785 + mtgA DVU2296 0.82 -0.9 2,390,788 - mtgA DVU2296 0.82 -1.4 2,390,917 + +0.9 2,390,931 + -0.3 2,390,931 + -1.2 2,390,944 - -1.3 2,390,956 + +1.9 2,390,997 - +0.4 2,391,006 - -0.8 2,391,046 + -1.3 2,391,046 + +0.0 2,391,054 - +0.5 2,391,165 - +0.3 2,391,226 + DVU2297 0.11 +2.6 2,391,242 - DVU2297 0.13 +2.2 2,391,291 + DVU2297 0.19 +2.7 2,391,305 - DVU2297 0.20 -2.9 2,391,314 + DVU2297 0.21 +2.9 2,391,777 + DVU2297 0.75 +2.8 2,391,846 + DVU2297 0.83 +2.8 2,391,900 + DVU2297 0.90 +2.9
Or see this region's nucleotide sequence